Effects of Acetylcholine Releasing Drugs on Electrical Activities Obtained From Auerbach's Plexus in the Guinea Pig Ileum

Satô, Takao; Takayanagi, Issei; Takagi, Keijiro

doi:10.1254/jjp.24.447

Cited by 22 publications

(4 citation statements)

References 20 publications

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“…Previous studies with extracellular recording from myenteric neurones, showed predominantly excitatory effects of 5-HT (Sato et al 1974;Dingledine et al 1974). Extracellular suction electrodes record activity almost exclusively from S-cells (Dingledine & Goldstein, 1976;North & Williams, 1977) and it is appropriate that the predominant response of S-cells to 5-HT in the present study was a membrane depolarization (Tables 1 & 2).…”

Section: Discussionmentioning

confidence: 82%

“…In contrast, 5-HT inhibits the peristaltic reflex evoked in isolated pieces of ileum (Kosterlitz & Robinson, 1957;Biilbring & Crema, 1958) and also inhibits the contraction of the ileum brought about by stimulating the intramural nerves (Gintzler & Musacchio, 1974). When myenteric neurones are induced to fire by contact with a fine suction electrode, 5-HT has predominantly excitatory effects (Sato, Takayanagi & Takagi, 1974;Dingledine, Godstein & Kendig, 1974), but when the membrane potential of myenteric neurones is recorded directly with intracellular electrodes, the consistent effect of 5-HT is presynaptic inhibition of acetylcholine release (Henderson & North, 1975;North, Henderson, Katayama & Johnson, 1980). However, in a proportion of cells 5-HT has direct effects on the membrane of the myenteric neurones (Henderson & North, 1975;Wood & Mayer, 1979).…”

Section: Introductionmentioning

confidence: 99%

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Multiple actions of 5‐hydroxytryptamine on myenteric neurones of the guinea‐pig ileum.

Johnson¹,

Katayama²,

North³

1980

The Journal of Physiology

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SUMMARY1. Intracellular recordings were made from neurones lying within ganglia isolated from the myenteric plexus of the guinea-pig ileum.2. 5-hydroxytryptamine (5-HT) (200 nM-10 4uM) was applied to 160 neurones by adding it to the perfusing solution. Depolarizations were observed in 27 % of neurones, and were more likely to be observed in S-cells. Hyperpolarizations were observed in 30 % of neurones, and were more likely to be seen in AH cells. 52 % of neurones were unaffected.3. 5-HT was applied to 104 neurones by micro-electrophoresis from an electrode with its tip placed close to the soma membrane. Depolarizations were observed in 35% of cells, and hyperpolarizations 13%; 10% of neurones showed biphasic responses in membrane potential and the remaining cells were unaffected.4. The depolarizing responses to 5-HT were associated with an increase in neuronal input resistance and were probably due to inactivation of the membrane potassium conductance. The responses declined in amplitude during prolonged or repeated applications of 5-HT.5. The hyperpolarizing responses to 5-HT were associated with a fall in neuronal input resistance and were probably due to activation of the membrane potassium conductance. These responses persisted throughout the duration of application of 5-HT and did not show marked tachyphylaxis. The hyperpolarizing responses were reversibly abolished by calcium-free solutions.6. The depolarizing responses to 5-HT may underlie the release of acetylcholine caused by 5-HT in the guinea-pig ileum. The hyperpolarizing responses may contribute to the inhibition of the peristaltic reflex which has been observed when 5-HT is applied to the serosal aspect of the intestine.

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Section: Discussionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Multiple actions of 5‐hydroxytryptamine on myenteric neurones of the guinea‐pig ileum.

Johnson¹,

Katayama²,

North³

1980

The Journal of Physiology

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“…Previous investigations have shown that cells of both the myenteric and submucous plexuses of the small and large intestine can be excited by ACh and other nicotinic agonists (Wood, 1970;Ohkawa & Prosser, 1972;Sato, Takayanagi & Takagi, 1974;North & Nishi, 1976). These cells demonstrate a fast synaptic input that is mediated by nicotinic receptors (Nishi & North, 1973;Hirst, Holman & Spence, 1974;Suprenant, 1984).…”

Section: Discussionmentioning

confidence: 99%

Motoneurones of the submucous plexus regulate electrical activity of the circular muscle of canine proximal colon.

Sanders¹,

Smith²

1986

The Journal of Physiology

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SUMMARY1. The hypothesis that the circular muscle of the canine proximal colon receives motor input from neurones in the submucous plexus was tested. Circular muscle cells were impaled with micro-electrodes and submucous plexus neurones were stimulated by electrical field stimulation and microejection of acetylcholine (ACh).2. In the presence of atropine to block the direct muscarinic effects, microejection of ACh onto the submucosa where intact submucous ganglia were suspended evoked:(i) an inhibitory junction potential (i.j.p.) that reduced the amplitude, duration and rate of rise of the subsequent slow wave; (ii) a slow wave of increased duration following the initial inhibitory response. These responses were enhanced by increasing the volume ofACh administered. Responses to ACh were blocked by hexamethonium, 10-4 M; d-tubocurarine, 10-4 M; or tetrodotoxin (TTX), 10-6 M, suggesting they were neural in origin.3. Both inhibitory and excitatory responses were the result of non-cholinergic and non-adrenergic nerves. The transmitters mediating these effects are unknown.4. Removal of the longitudinal muscle, myenteric plexus, and the serosal portion of the circular muscle had no apparent effect on the responses to application of ACh to submucosal ganglia. In these preparations the responses to field stimulation were identical to those produced by ACh.5. The submucous plexus also provides cholinergic input to the circular muscle. When ACh was discretely applied to the submucosa cholinergic responses were elicited at the muscle cell which were significantly reduced by hexamethonium or TTX. These findings suggest that the cholinergic responses were the result of ACh release by neurones at the effector and not by overflow of the exogenous ACh. Cholinergic responses were also elicited in preparations in which the myenteric plexus had been removed.6. Slow waves in circular muscle ofthe proximal colon yield excitation-contraction coupling in the absence of Ca2+ action potentials. Therefore the influence of submucous neurones on electrical slow waves has direct consequences on motor activity. Reduction in the amplitude and duration of slow wave by i.j.p.s results in reduction in the amplitude and duration of phasic contractions. Excitatory inputs enhance contractions. K. M. SANDERS AND T. K. SMITH7. The data support a new concept: motoneurones emanating from submucous ganglia innervate the circular muscle and provide inhibitory and excitatory inputs to regulate slow wave activity. There appear to be at least three types of motoneurones activated by nicotinic stimulation of the ganglia or by field stimulation: (i) nonadrenergic inhibitory nerves, (ii) cholinergic excitatory nerves, and (iii) noncholinergic, non-adrenergic excitatory nerves. These nerves may regulate colonic motility patterns.

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“…Fine suction electrodes were used by Dingledine and co-workers (41)(42)(43), North and Williams (160,161), and Sato et al (186,187), while the others used either metal or glass pipette electrodes for single-unit recording. The group in Leningrad was the first to record from myenteric neurons with the intestine in situ and blood flow intact (73,110,164).…”

mentioning

confidence: 99%

Electrical and synaptic behavior of enteric neurons

Wood

1989

Comprehensive Physiology

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Effects of Acetylcholine Releasing Drugs on Electrical Activities Obtained From Auerbach's Plexus in the Guinea Pig Ileum

Cited by 22 publications

References 20 publications

Multiple actions of 5‐hydroxytryptamine on myenteric neurones of the guinea‐pig ileum.

Multiple actions of 5‐hydroxytryptamine on myenteric neurones of the guinea‐pig ileum.

Motoneurones of the submucous plexus regulate electrical activity of the circular muscle of canine proximal colon.

Electrical and synaptic behavior of enteric neurons

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