Effects of acid pre-treatment on bio-hydrogen production and microbial communities during dark fermentation

Ultrasonic pretreatment of palm oil mill effluent: Impact on biohydrogen production, bioelectricity generation, and underlying microbial communities

“…and Thermoanaerobacterium spp. in bioH 2 producing reactors could even be close to 80% [16] and 50% [17], respectively.…”

“…FISH tests were carried out using following fluorescently labeled oligonucleotide probes: MPB1 for methanogens [15], P932 for Clostridium spp. of clusters I and XI [16], Tbm1282 for genus Thermoanaerobacterium [17], EUB338 for domain Bacteria [18], and ARC915 for domain Archaea [19]. Hybridization conditions were optimized according to Amann et al [18].…”

“…Dark fermentation is traditionally known as anaerobic digestion wherein H 2 is produced by acidogenic bacteria as an intermediate that can further be reduced to methane by the methanogenic microflora [16]. In order to enhance bioH 2 production, such approaches including but not limited to heat shock, acidebase treatment, and ultrasonication of inocula [9,10], have been explored to diminish methanogenic activity.…”

Enhancing fermentative hydrogen production from sucrose

“…Even though hydrogen is currently being produced utilizing fossil fuels, several recent studies have reported on the feasibility of biological processes for producing hydrogen from organic feedstocks -biohydrogen. Recent research has demonstrated that, with appropriate pretreatment of the feed such as acid, alkaline, heat (Lee et al, 2009;Kim and Lee, 2010;Valdez-Vazquez et al, 2006) to inhibit hydrogen-consuming methane-formers, the classical anaerobic process can be engineered to harvest hydrogen from organic substrates instead of methane -a process referred to as dark fermentation (DF). When sucrose is used as the feedstock in dark fermentation, the maximum fermentative hydrogen yield is 8 mol H 2 /mol sucrose when acetic acid is the aqueous end product (DG°= À457.5 kJ).…”

Codigestão anaeróbia de resíduos orgânicos

“…Existem vários métodos disponíveis para o tratamento de resíduos orgânicos putrescíveis, porém a digestão anaeróbia parece ser uma promissora alternativa tecnológica (LEE et al, 2009).…”