Effects of Activated Charcoal on Growth and Morphogenesis in Cell Cultures

Fridborg, Gunnar; Eriksson, Tage

doi:10.1111/j.1399-3054.1975.tb03843.x

Cited by 121 publications

(47 citation statements)

References 3 publications

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“…To increment the development of the plantlets, activated charcoal was added to the medium at the last stage. Addition of activated charcoal have been reported for other species such as Anemone canadensis, Anemone hupehensis, Nicotiana tabacum (Johansson, 1983), Allium cepa, Daucus carota and Haplopappus gracilis (Fridborg and Erikson, 1975;Fridborg et al, 1978). Favourable effects of its addition to the medium were probably due the adsorption of inhibiting substances derived from the agar (Kohlenbach and Wernicke, 1978), auxins and cytokinins (Weatherhead et al, 1978), chelates (HeberleBors, 1980) and phenolic substances (Fridborg et al, 1978).…”

Section: Resultsmentioning

confidence: 99%

In vitro culture of Vriesea gigantea and Vriesea philippocoburgii: two vulnerable bromeliads native to Southern Brazil

Droste

Silva

Matos

et al. 2005

Braz. arch. biol. technol.

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Micropropagation studies were carried out using the seeds for establishing an in vitro culture of Vriesea gigantea and Vriesea philippocoburgii. Germination rate of V. gigantea was higher than of V. philippocoburgii. Plantlets of V. philippocoburgii gave rise to many adventitious shoots when cultivated in Knudson basal medium. In contrast, for V. gigantea, a higher salts-concentration was needed, so that the number of shoots was increased by Murashige and Skoog medium. Addition of activated charcoal and naphthaleneacetic acid in regeneration medium allowed the growth of shoots and the formation of roots, confirming the success of in vitro culture. The differences in expression of the genotypes reinforce the need of more research in order to set up the conditions that could offer the best response of the specific tissues.

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Section: Resultsmentioning

confidence: 99%

In vitro culture of Vriesea gigantea and Vriesea philippocoburgii: two vulnerable bromeliads native to Southern Brazil

Droste

Silva

Matos

et al. 2005

Braz. arch. biol. technol.

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“…In cork oak, this has been significantly promoted by the effect of activated charcoal added to the culture medium. Nevertheless, in some cases growth inhibition has also been reported (Fridborg and Eriksson, 1975;Fridborg et al, 1978), also with embryos from anther origin (Johansson, 1983). The positive effect of 1% activated charcoal on stopping secondary somatic embryogenesis and promoting somatic embryo growth and later germination has been observed in other woody species, such as Taxus brevifolia (Chee, 1996) and Larix × marschlinsii (Von Aderkas et al, 2002).…”

Section: Effect Of Amino Acids and Activated Charcoal On Embryo Growthmentioning

confidence: 97%

Oak somatic and gametic embryos maturation is affected by charcoal and specific aminoacids mixture

Pintos¹,

Manzanera

Bueno³

2010

Ann. For. Sci.

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Abstract• Development of both somatic and gametic embryogenesis has many applications in clonal forestry and genetic improvement, for instance as mass-propagation of genetically improved plants and production of pure lines through doubled-haploid plant regeneration from gametic embryos.• The goal of this work was to improve growth, maturation and plantlet regeneration of cork oak (Quercus suber L.) embryos from both somatic and gametic origin.• Activated charcoal promoted a significant increase in growth in terms of relative size and weight of both somatic and doubled-haploid embryos, as well as a more efficient control of secondary somatic embryogenesis during development. A significant interaction was also observed with amino acid nutrition. While some amino acids (i.e., glutamine, arginine or asparagine) did not show significant differences with the controls, a mixture of these three amino acids or gamma amino butyric acid stimulated embryo growth. The highest survival rate during acclimation of plantlets from both somatic and doubled-haploid origin was obtained when the embryos had been previously cultured on basal medium with 3% sucrose and 1% activated charcoal for two months at 4 • C and germinated on medium supplemented with 6-benzylaminopurine and indole-3-butyric acid.• We obtained more than 900 cork oak plantlets acclimated from several embryogenic lines, with a high survival rate, demonstrating that this methodology is applicable for large scale plantlet production. We also report the first regeneration of doubled-haploid plantlets in cork oak. • Le développement des deux embryogenèses gamétique et somatique a de nombreuses applications dans la foresterie clonale et l'amélioration génétique, par exemple en tant que propagation en masse de plants génétiquement améliorés et la production de lignées pures par régénération de plants haploïdes doublés d'embryons gamétiques. Mots-clés• L'objectif de ce travail était d'améliorer la croissance, la maturation et la régénération des plantules des embryons de chêne-liège (Quercus suber L.) des deux origines gamétique et somatique.• Le charbon actif a favorisé une augmentation significative de la croissance en termes de taille et de poids des embryons haploïdes doublés et somatiques, ainsi qu'un contrôle plus efficace de l'embryogenèse somatique secondaire au cours du développement. Une interaction significative a également été observée avec la nutrition aminoacide. Alors que certains acides aminés (c'est-à-dire, la glutamine, l'arginine ou l'asparagine) ne montrent pas de différences significatives avec les témoins, un mélange de ces trois acides aminés ou l'acide butyrique amino gamma a stimulé la croissance de l'embryon. Le taux de survie le plus élevé au cours de l'acclimatation des plantules d'origine à la fois somatique et haploïde doublé, a été obtenu lorsque les embryons ont été auparavant cultivés sur milieu de base à 3 % de saccharose et de 1 % de charbon actif pendant deux mois à 4 • C et ont germé sur un milieu complété par la 6-benzylaminopurine et ...

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“…The addition of activated charcoal is sometimes added to culture media where it may have either a beneficial or deleterious effect. Growth and differentiations were stimulated in orchids [15], onions and carrots [16,17], tomatoes [18]. On the other hand, an inhibition of cell growth was noticed on addition of activated charcoal to culture medium of soybean [17].…”

Section: Undefined Organic Supplementsmentioning

confidence: 96%