Effects of Active Components of Fuzi and Gancao Compatibility on Bax, Bcl‐2, and Caspase‐3 in Chronic Heart Failure Rats

Wang, Liqin; Zhang, Yuyan; Zhou, Huifen; Yu, Li; Yang, Jiehong; Wan, Haitong

doi:10.1155/2016/7686045

Cited by 21 publications

(22 citation statements)

References 45 publications

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“…AR plays an important role in the carcinogenesis and progress of prostate cancer; increased levels of AR expression predict the metastatic progression of prostate cancer4647. Bcl-2 was the upstream protein of caspase3, and they are most relevant to cell apoptosis, and Caspase-3 is the major enzyme cleavage effector in the process of cell apoptosis and Bcl-2 is the main anti-apoptotic agent and the anti-oxidative protein is an inhibitor of cell apoptosis48. Both Caspase-3 and Bcl-2 play an important role in signal transduction of cell apoptosis49.…”

Section: Discussionmentioning

confidence: 99%

Screening the active compounds of Phellodendri Amurensis cortex for treating prostate cancer by high-throughput chinmedomics

Zhang

Wang

et al. 2017

Sci Rep

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Screening the active compounds of herbal medicines is of importance to modern drug discovery. In this work, an integrative strategy was established to discover the effective compounds and their therapeutic targets using Phellodendri Amurensis cortex (PAC) aimed at inhibiting prostate cancer as a case study. We found that PAC could be inhibited the growth of xenograft tumours of prostate cancer. Global constituents and serum metabolites were analysed by UPLC-MS based on the established chinmedomics analysis method, a total of 54 peaks in the spectrum of PAC were characterised in vitro and 38 peaks were characterised in vivo. Among the 38 compounds characterised in vivo, 29 prototype components were absorbed in serum and nine metabolites were identified in vivo. Thirty-four metabolic biomarkers were related to prostate cancer, and PAC could observably reverse these metabolic biomarkers to their normal level and regulate the disturbed metabolic profile to a healthy state. A chinmedomics approach showed that ten absorbed constituents, as effective compounds, were associated with the therapeutic effect of PAC. In combination with bioactivity assays, the action targets were also predicted and discovered. As an illustrative case study, the strategy was successfully applied to high-throughput screening of active compounds from herbal medicine.

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Section: Discussionmentioning

confidence: 99%

Screening the active compounds of Phellodendri Amurensis cortex for treating prostate cancer by high-throughput chinmedomics

Zhang

Wang

et al. 2017

Sci Rep

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“…Akt is the central link of this pathway, being not only the direct target of the downstream, but also the most important target protein, which has protective effects on myocardial tissue damage induced by various factors [30]. Caspase-3 is the key executor and effector in the process of apoptosis, plays an important role in inducing apoptosis of cardiomyocytes, and has gradually become a potential target for antiapoptotic therapy [31]. e process of cardiomyocyte apoptosis involves very complex molecular biological mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Experimental Study on the Effect of Aconite and Angelica sinensis on Myocardial Ischemia Rats with Yang Deficiency and Blood Stasis

Cao

Xiao-dong

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Objective. To investigate the intervention effect and mechanism of Aconite and Angelica sinensis on myocardial ischemia rats with Yang deficiency and blood stasis. Methods. SPF-class SD rats were randomly divided into low-dose and high-dose groups. Each group was divided into control group, model group, and drug-administered group (FZ, DG, FG; 1 : 0.5, 1 : 1, 1 : 2). A rat model was prepared by intraperitoneal injection of hydrocortisone and isoproterenol plus cold stimulation. Each group was given corresponding decoction or distilled water for 14 days. The behavioral changes of rats in each group were observed. The morphological changes of rats cardiomyocytes were observed by HE staining. The average optical density (MOD value) and percentage of positive cells of Bcl-2, Bax, and Akt were determined by immunohistochemical staining method, and PEIs were calculated. Western blot and RT-PCR were used to determine the expression of PI3K, Caspase-3, Akt protein, and gene expression. Results. The compatibility of Aconite and Angelica sinensis improved the morphology of rat cardiomyocytes, increased the PEI values of Akt and Bcl-2 protein, and decreased the PEI values of Bax protein (P<0.01). The compatibility reduced the expression of Caspase-3 protein of rat myocardium and increased the protein expression of p-Akt, PI3K, and p-PI3K (P<0.01). The compatibility also significantly reduced the expression of Caspase-3 mRNA and increased the expression of PI3K mRNA and Akt mRNA (P<0.05 or P<0.01), and the effect of high-dose FG (1 : 2) group is the best. Conclusions. The method of preparing a rat model of myocardial ischemia with Yang deficiency and blood stasis was feasible. The compatibility of Aconite and Angelica sinensis reduced myocardial fibrosis and inflammatory reaction, protected ischemic cardiomyocytes, and reduced myocardial injury, whose mechanism may be related to the regulation of PI3K/Akt pathway. The compatible group had better intervention effects than Aconite or Angelica sinensis alone. The best one was high-dose FG (1 : 2).

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“…21,22 Fuzi (Radix Aconiti Lateralis Preparata) is proven to have cardiotonic action and reverse the dysfunction in CHF processing. 23,24 Although the pharmacological mechanisms underlying the actions of Gua Lou Shi (FructusTrichosanthis), Sang Bai Pi (Cortex Mori), and Tinɡ Li Zi (Semen Lepidii) remain unclear, their functions in dealing with phlegm retention are abundantly documented in the "Compendium of Materia Medica" (BencaoGangmu), an ancient TCM herbal text book.…”

Section: Discussionmentioning

confidence: 99%

Adjuvant therapy with traditional Chinese medicine in a heart failure patient complicated by hospital-acquired pneumonia: A case report

Chen

Chuang

Huang

et al. 2019

Complementary Therapies in Medicine

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Objective: We report a case of congestive heart failure complicated by hospital-acquired pneumonia that was successfully treated with traditional Chinese medicine (TCM) and antibiotics. Clinical features and outcome: A 33-year-old man with a history of heart failure developed pneumonia during hospitalization. After the standard antibiotic therapy for 3 days, he continued to experience persistent fever and progressive cough with purulent sputum. Broad spectrum antibiotics did not relieve the fever or the purulent sputum; therefore, the patient requested TCM for integrated therapy, and was subsequently treated with a regiment of "clearing heat and damp excreting" decoction according to TCM theory. After three days of TCM combination therapy, the pneumonia patches significantly improved on chest X-ray. His sputum was obviously decreased in amount and the fever was complete remission in the 5 th day of TCM adjuvant therapy. Conclusion: Integrated therapy with a "clearing heat and damp excreting" decoction may have improved hospital-acquired pneumonia in a patient comorbid with congestive heart failure. The anti-pyretic, anti-inflammatory, antitussive and diuretic effects of TCM may be responsible for the observed improvement. Further experimental studies are warranted to confirm the efficacy and mechanism of TCM action in the treatment of pneumonia.

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Effects of Active Components of Fuzi and Gancao Compatibility on Bax, Bcl‐2, and Caspase‐3 in Chronic Heart Failure Rats

Cited by 21 publications

References 45 publications

Screening the active compounds of Phellodendri Amurensis cortex for treating prostate cancer by high-throughput chinmedomics

Screening the active compounds of Phellodendri Amurensis cortex for treating prostate cancer by high-throughput chinmedomics

Experimental Study on the Effect of Aconite and Angelica sinensis on Myocardial Ischemia Rats with Yang Deficiency and Blood Stasis

Adjuvant therapy with traditional Chinese medicine in a heart failure patient complicated by hospital-acquired pneumonia: A case report

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