The function of the tendon is to connect bones and muscles, transmit the force generated by muscle contraction to the bones, cause joint activity and body movement, and maintain the stability of the joints. Due to excessive use of body impact in the game, the phenomenon of fouling by teammates, resulting in tendon injury and muscle injury, is a common type of sports injury in sports training and daily life, often causing pain and affecting the body's normal ability to coordinate sports. Or even lead to changes in joint load patterns. Therefore, this article studies the biological characteristics of tendon stem cells in people who have been injured by muscle collision in the foul play. In this study, 30 cases of normal tendon stem cells and 10 cases of tendon stem cells from injured muscles were prepared for cell printing. All specimens were not treated with any anti-tumor treatment before surgery. Fix the sample with 3% formaldehyde solution, rinse with distilled water repeatedly, and dry in a clean space. The samples were dried and placed on the AFM stage, using IX-71 inverted phase contrast microscope to observe the dispersion of cells, select cells for scanning. Select the Tapping mode, adjust the laser to coincide with the tip of the needle, and place the laser spot of the detector on the center dot. Perform automatic frequency modulation in the operating software and adjust the probe until the needle tip touches the sample to start scanning. The experimental data shows that the average size of normal tendon stem cells is 221.11nm*173.88nm, and the average size of tendon stem cells of muscle collision injury is 223.75nm*267.73nm. The experimental results showed that the normal tendon stem cells were fresh under the microscope, and the tendon cells were evenly arranged; while the collagen fibers of the tendon stem cells of the injured muscles were partially lysed, and the tendon cells were disorderly arranged.