Anaerobic exercise capacity and neuromuscular activity research is an important content and an emerging research field in sports training research. At present, the understanding of anaerobic exercise ability in academia is still at the general and overall cognitive level, and the understanding and application of anaerobic exercise ability cannot fully meet the needs of competitive sports practice. In order to solve these problems, this paper proposes a cyclic anaerobic exercise performance and neuromuscular activity based on artificial intelligence genetic algorithm, aimed at studying the theory and application mechanism of anaerobic exercise capacity and neuromuscular activity and its application characteristics in competitive sports practice. The approach in this paper is to design genetic operators, compare artificial intelligence genetic algorithms, and test neuromuscular movements. The purpose of these methods is to provide exercisers with a feasible and more effective new method of daily training and to investigate whether this new training method can optimize anaerobic exercise in humans. In this paper, by studying the kinematic basis of anaerobic exercise capacity and the mechanism of neuromuscular regulation, a model of muscle neuron population in anaerobic exercise is established. The results showed that the CMC of the beta band was significantly higher than that of the alpha band at the same strength level, with a difference of 0.03.