Effects of acute dimethoate administration on antioxidant status of liver and brain of experimental rats

Sharma, Yukti; Bashir, Somia; Irshad, Mohammad; Gupta, Sunil; Dogra, Tirath Das

doi:10.1016/j.tox.2004.06.062

Cited by 173 publications

(114 citation statements)

References 19 publications

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“…In humans, Banerjee et al (1999) observed increased levels of LPO and ROS scavenging enzymes in blood samples of OP-poisoned subjects, and Ranjbar et al (2002) reported increased levels of erythrocyte LPO and reduced anti-oxidant capacity in OP pesticide manufacturing workers. In vitro exposure to chlorpyrifos resulted in the induction of erythrocyte LPO (Gultekin et al, 2000); while Sharma et al (2005) reported that dimethoate exposure in rats increased the generation of free radicals in liver and brain, and Fortunato et al (2006) showed that LPO in brain increased after acute malathion exposure in rats. Finally, Debnath and Mandal (2000) observed that quinalphos caused damage and degeneration of testicular tissue in rats due to free-radical mediated LPO.…”

Section: Discussionmentioning

confidence: 99%

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Methyl-parathion decreases sperm function and fertilization capacity after targeting spermatocytes and maturing spermatozoa

Piña-Guzmán

Sánchez-Gutiérrez

Marchetti

et al. 2009

Toxicology and Applied Pharmacology

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Paternal germline exposure to organophosphorous pesticides (OP) has been associated with reproductive failures and adverse effects in the offspring. Methyl parathion (Me-Pa), a worldwide-used OP, has reproductive adverse effects and is genotoxic to sperm.Oxidative damage has been involved in the genotoxic and reproductive effects of OP.The purpose of this study was to determine the effects of Me-Pa on spermatozoa function and ability to fertilize. Male mice were exposed to i.p.) and spermatozoa from epididymis-vas deferens were collected at 7 or 28 days posttreatment (dpt) to assess the effects on maturing spermatozoa and spermatocytes, respectively. DNA damage was evaluated by nick translation (NT-positive cells) and SCSA (%DFI); lipoperoxidation (LPO) by malondialdehyde production; sperm function by spontaneous-and induced-acrosome reactions (AR); mitochondrial membrane potential (MMP) by using the JC-1 flurochrome; and, fertilization ability by an in vitro assay and in vivo mating. Results showed alterations in DNA integrity (%DFI and NTpositive cells) at 7 and 28 dpt, in addition to decreased sperm quality and a decrease in induced-AR; reduced MMP and LPO was observed only at 7 dpt. We found negative correlations between LPO and all sperm alterations. Altered sperm functional parameters were associated with reduced fertilization rates at both times, evaluated either in vitro or in vivo. These results show that Me-Pa exposure of maturing spermatozoa and spermatocytes affects many sperm functional parameters that result in a decreased fertilizing capacity. Oxidative stress seems to be a likely mechanism of the detrimental effects of Me-Pa in male germ cells.Key words. methyl-parathion, sperm function, fertilization, oxidative stress.

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Section: Discussionmentioning

confidence: 99%

“…More recently, it has been reported that OP produce oxidative stress in different tissues, such as liver, blood and brain (Akhgari et al, 2003;Sharma et al, 2005;Fortunato et al, 2006) through the formation of reactive oxygen species (ROS) (Banerjee et al, 1999;Ranjbar et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Methyl-parathion decreases sperm function and fertilization capacity after targeting spermatocytes and maturing spermatozoa

Piña-Guzmán

Sánchez-Gutiérrez

Marchetti

et al. 2009

Toxicology and Applied Pharmacology

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“…immunotoxicity (Galloway and Handy, 2003). Furthermore, Organophosphorus insecticides exert their biological effects through electrophilic attack on the cellular constituents of hepatic and brain tissues (Samanta and Chainy, 1995) with simultaneous generation of reactive oxygen species (Sharma et al, 2005). Reactive Oxygen Species have been implicated in hepato and neurotoxicity induced by several organophosphorus (Bagchi et al, 1995;Mansour and Mossa, 2010; and is associated with lipid peroxidation and phospholipids degradation (Mansour and Mossa, 2009).…”

Section: Introductionmentioning

confidence: 99%

Protective Role of Vitamin C and Green Tea Extract on Malathion-Induced Hepatotoxicity and Nephrotoxicity in Rats

Elzoghby¹,

Hamoda²,

Aboubakr³

et al. 2014

American Journal of Pharmacology and Toxicology

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The present study was designed to determine the modulating effect of green tea and vitamin C against adverse effects of malathion. Animals were divided into four groups 5 rats /group). Group one was used as a control. Group two given malathion (50 mg/kg/day; 1/50 of the LD50 for four weeks). Group three and Group four were given malathion (50 mg/kg/day; 1/50 of the LD50 for four weeks) plus vitamin C (200 mg/kg/day) and plus green tea (36 mg/kg/day) respectively. At the end of the fourth week, the malathion-treated group had significantly lower Red Blood Cell count (RBCs), Hemoglobin concentration (Hb), Packed Cell Volume (PCV%) and leucocytes (WBCs) than the control group. Compared to the control group, the malathion-treated group had significantly higher serum Alkaline Phosphatase (ALP), Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Lactate Dehydrogenase (LDH), urea, creatinine and uric acid levels than the control group. The malathion treated rats also had significantly lower serum total protein, albumin and globulin levels than the control group, but the malathion plus vitamin C and malathion plus green tea groups did not differ from the control group in terms of these parameters. Moreover, concomitant vitamin C and green tea treatment significantly normalized, at least partially, all of the other hematological and biochemical parameters that were altered by malathion. Liver tissue homogenate in malathion treated group had lower Glutathione (GSH), Glutathione Peroxidase (GSH-PX) and Superoxide Dismutase (SOD) levels accompanied with higher level of Malondialdehyde (MDA) than the control group. Histopathological studies revealed that the malathion-treated, malathion plus vitamin C and malathion plus green tea treated groups exhibited histopathological changes in liver and kidney tissues, although some pathological features were only observed in the malathion-treated group. Thus, vitamin C and green tea can reduce malathion hepatotoxicity and nephrptoxicity.

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“…Bifenthrin belong to pyrethroids pesticides those are extremely dangerous to fish because at the nerve cell ending it interfere with Na + channel gating as well as with other ions channels like Cl − and Ca 2+ (Burr and Ray, 2004). Pesticide stimulated oxidative stress has become increasingly reported from the last few decades in toxicological research as a possible mechanism of toxicity (Ciccheti and Argentin, 2003;Sharma et al, 2005). To save the biomolecules from the injurious effects of reactive oxygen species (ROS) resulting from the breakdown of different toxicants, fish possess an antioxidant defense system.…”

Section: Introductionmentioning

confidence: 99%

Pesticides Mixture Toxicity; Effects on Superoxide Dismutase Activity in Indian Major Carps

Naz¹

2017

PAKJAS

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The current study was conducted to determine the tolerance limits of Indian major carps viz. Catla catla, Cirrhina mrigala and Labeo rohita against pesticides mixture (Bifenthrin + Chlorpyrifos + Endosulfan) and to see it effect on antioxidant enzyme like superoxide dismutase (SOD) activity in the liver of these species. The 96-hr LC 50 and lethal concentration of pesticides mixture (BIF+CPF+END) for C. catla, C. mrigala and L. rohita was determined in the static bioassay methods as 1.09, 1.81 1.63 and 1.90, 2.78, 2.30 μgL -1 , respectively.C. catla exhibited higher sensitivity toward tertiary pesticides mixture followed by L. rohita and C. mrigala. The activity of SOD in the liver of all three fish species exposed to pesticides mixture was significantly (p<0.05) higher as compare to control fish. The SOD activity showed time dependent increasing trend in the liver of all three fish species.

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Effects of acute dimethoate administration on antioxidant status of liver and brain of experimental rats

Cited by 173 publications

References 19 publications

Methyl-parathion decreases sperm function and fertilization capacity after targeting spermatocytes and maturing spermatozoa

Methyl-parathion decreases sperm function and fertilization capacity after targeting spermatocytes and maturing spermatozoa

Protective Role of Vitamin C and Green Tea Extract on Malathion-Induced Hepatotoxicity and Nephrotoxicity in Rats

Pesticides Mixture Toxicity; Effects on Superoxide Dismutase Activity in Indian Major Carps

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