Effects of acute handling stress on cerebral monoaminergic neurotransmitters in juvenile Senegalese sole <i>Solea senegalensis</i>

Weber, R.; Maceira, Jorge José Pérez; Aldegunde, M.; Peleteiro, J.B.; Martín, L.

doi:10.1111/jfb.12774

Cited by 7 publications

(7 citation statements)

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“…In fish, Npy has a role in the regulation of feeding (orexigenic) and growth, as well as in the stimulatory control of reproductive processes (Zohar et al ; Shahjahan, Kitahashi, & Parhar, ). Dopamine is an inhibitory system in the control of reproduction in several fish species, including sole (Guzmán et al ) and, in this species, it also appears to participate in responses to challenging stress situations (Costas et al ; Weber et al ). Interestingly, GnIH has a role not only in the control of reproduction, but also in the regulation of food intake and in the mediation of stress responses (Tsutsui, ; Tsutsui & Ubuka, ; Ernst, Lynn, & Bentley, ).…”

Section: Discussionmentioning

confidence: 99%

Gonadotropin‐inhibitory hormone in the flatfish, Solea senegalensis: Molecular cloning, brain localization and physiological effects

Aliaga-Guerrero

Paullada-Salmerón

Piquer

et al. 2017

J of Comparative Neurology

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Recently, gonadotropin-inhibitory hormone (GnIH) has emerged as an important regulator of reproduction in birds and mammals. This RFamide neuropeptide has neuromodulatory functions and controls the synthesis and/or release of gonadotropin-releasing hormone (GnRH) and gonadotropins. Although teleosts represent about half of all living vertebrates, scientific and technological advances on the Gnih system in fish are scarce, contradictory, and inconclusive. Research on the fish Gnih system appears necessary to better clarify its role in the neuroendocrine and environmental control of vertebrate reproduction. In this study, we cloned a full-length sequence for the Gnih precursor of a flatfish, the Senegalese sole, coding for three putative Gnih peptides (ssGnih). We also generated specific antibodies against these ssGnih peptides, and used them to localize Gnih cells and their projections in the brain and pituitary. The expression of gnih was particularly evident in the diencephalon, but also in the olfactory bulbs/cerebral hemispheres, optic tectum/tegmentum, retina, and pituitary. The three antibodies used provided consistent results and showed that ssGnih-immunoreactive perikarya were present in the olfactory bulbs, ventral telencephalon, caudal preoptic area, dorsal tegmentum and rostral rhombencephalon, and their fibers innervated the brain and pituitary profusely. Intramuscular injection of ssGnih-3 provoked a significant reduction in gnrh-3 and lh expression, whereas ssGnih-2 treatment did not affect transcript levels of the main reproductive genes. Our results reveal the existence of a functional Gnih system in the sole brain, profusely innervating different brain areas and the pituitary gland, which could represent an important factor in the neuroendocrine control of flatfish reproduction.

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Section: Discussionmentioning

confidence: 99%

Gonadotropin‐inhibitory hormone in the flatfish, Solea senegalensis: Molecular cloning, brain localization and physiological effects

Aliaga-Guerrero

Paullada-Salmerón

Piquer

et al. 2017

J of Comparative Neurology

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“…Dopamine and norepinephrine are involved in the organization of stress response in vertebrates (Winberg & Nilsson, ). Recent research shows that the brain dopamine level in teleosts increased after being stressed by chasing, handling and air exposure (Gesto et al ., ; Weber et al ., ). Furthermore, Wolkers et al .…”

Section: Discussionmentioning

confidence: 99%

“…Dopamine and norepinephrine are involved in the organization of stress response in vertebrates (Winberg & Nilsson, 1993). Recent research shows that the brain dopamine level in teleosts increased after being stressed by chasing, handling and air exposure (Gesto et al, 2013;Weber et al, 2015). Furthermore, Wolkers et al (2015) demonstrated that territorial resident matrinxã fish (Brycon amazonicus) have a high dopamine level in the hypothalamus of the brain after being subjected to a social challenge stress by introducing an intruder into their territory.…”

Section: Discussionmentioning

confidence: 99%

“…The blood glucose concentration and the level of dopaminergic activity are widely used indicators of stress responses in fish (Gesto et al, 2013;Iwama et al, 1995;Weber et al, 2015;Wells & Pankhurst, 1999). This work reported that blood glucose increased because of stress in medaka males (Shimomura et al, 2019), but no information on dopamine synthesis in the brain of stressed medaka fish is available.…”

Section: Introductionmentioning

confidence: 99%

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The presence of a conspecific induces risk‐taking behaviour and enlargement of somata size of dopaminergic neurons in the brain of male medaka fish

Otsuka

Shimomura

Niwa

et al. 2020

Journal of Fish Biology

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Boldness and risk‐taking behaviours in animals are important traits to obtain advantages such as habitation, food resources, reproductive success and social dominance. Risk‐taking behaviour is influenced by physiological and environmental conditions; however, whether individual fish become bolder by the presence of conspecifics remains unknown. In this study, a light–dark preference test was conducted using medaka fish (Oryzias latipes) with or without a neighbouring conspecific. It was found that individual medaka male fish preferred a light environment and avoided a dark environment, whereas the display of a neighbouring conspecific enhanced the time the male spent in the dark environment (i.e., this condition encouraged risk‐taking). The blood glucose level increased in fish confined to the dark condition but did not increase in light‐preferring fish and risk‐taking fish. Large somata expressing tyrosine hydroxylase, which is the rate‐limiting enzyme in dopamine synthesis, were detected in the telencephalic and diencephalic brain regions in risk‐taking medaka, whereas large somata were detected in the diencephalic region in medaka confined to the dark condition. These findings indicated that medaka is a good fish model to explore the central roles of dopaminergic neurons in the telencephalon and the diencephalon, which regulate risk‐taking behaviour.

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“…The fish were suspended in the air for 3 min and were then redistributed in 50 L tanks (two fish/tank). At intervals of 30 min, 2 and 24 h after air exposure, the fish were caught (in pairs, within 15 s) and finally placed in a 10 L tank containing the anaesthetic MET (10 mg/L) before blood sampling [21]. MET was used at this stage for the following reasons: 1) the dose used induces deep anaesthesia within 1.5 min [20], and 2) in S. senegalensis it produces a weaker stress response than other fish anaesthetics and it does not significantly affect cortisol levels 2 and 24 h after exposure [8].…”

Section: Experimental Designmentioning

confidence: 99%

Stress-preventing effects of the anaesthetic agents 2-phenoxyethanol, MS-222, clove oil and metomidate in the Senegalese sole Solea senegalensis

Weber¹,

Maceira²,

Aldegunde³

et al. 2017

JCLM

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The journal implements double-blind peer review practiced by specially invited international editorial board members. Objective: To determine the effectiveness of the anaesthetic agents 2-phenoxyethanol, MS-222, clove oil and metomidate in attenuating acute handling stress in juvenile specimens of Solea senegalensis subjected to two routine stressful events specific to aquaculture and/or fish research. Methods: The stress-preventing effects of four anaesthetic agents (2-phenoxyethanol, 600 mg/L; metomidate, 5 mg/L; clove oil, 30 mg/L and MS-222, 75 mg/L) were evaluated in juvenile specimens of Senegalase sole (Solea senegalensis) subjected to two different types of acute (handling-related) stress: air exposure and net handling (chasing). To assess the stress-preventing effects of the four anaesthetic agents, diverse blood and plasma parameters (haematocrit, haemoglobin, glucose, lactate and cortisol levels) were determined as stress indicators. Fish were treated with the anaesthetic agents before being subjected to the different types of acute stress, and they were sacrificed 30 min, 2 and 24 h later. Control fish were processed in the same way without pretreatment with the anaesthetic agents. Results: The net handling stress was of sufficient intensity to cause a significant increase in the levels of most of the stress indicators considered. By contrast, air exposure stress only induced significant increases in cortisol and haemoglobin levels. Conclusions: The stress-preventing effects of the anaesthetic agents tested were ranked on the basis of their capacity to prevent increases in the haematocrit, haemoglobin, glucose, lactate and cortisol levels, as follows: metomidate (5 mg/L) > clove oil (30 mg/L) > MS-222 (75 mg/L) > 2-phenoxyethanol (600 mg/L).

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Effects of acute handling stress on cerebral monoaminergic neurotransmitters in juvenile Senegalese sole Solea senegalensis

Cited by 7 publications

References 54 publications

Gonadotropin‐inhibitory hormone in the flatfish, Solea senegalensis: Molecular cloning, brain localization and physiological effects

Gonadotropin‐inhibitory hormone in the flatfish, Solea senegalensis: Molecular cloning, brain localization and physiological effects

The presence of a conspecific induces risk‐taking behaviour and enlargement of somata size of dopaminergic neurons in the brain of male medaka fish

Stress-preventing effects of the anaesthetic agents 2-phenoxyethanol, MS-222, clove oil and metomidate in the Senegalese sole Solea senegalensis

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