Effects of acute unilateral renal denervation in the rat.

Bello‐Reuss, Elsa; Colindres, Rómulo E.; Pastoriza-Munoz, E; Ra, Mueller; Gottschalk, Carl W.

doi:10.1172/jci108069

Cited by 215 publications

(110 citation statements)

References 31 publications

Supporting

Mentioning

100

Contrasting

Unclassified

Order By: Relevance

“…Animals were anaesthetized with methohexital (75 mg/kg i.p.) and denervated according to the method of Bello-Reuss et al [3]. The abdominal wall was opened along the midline and the left renal artery and vein were exposed by carefully retracting the adipose tissue and the peritoneum.…”

Section: Renal Denervationmentioning

confidence: 99%

Influence of dietary NaCl intake on renin gene expression in the kidneys and adrenal glands of rats

et al. 1993

View full text Add to dashboard Cite

Abstract. The aim of this study was to examine the influence of dietary NaC1 intake on renin gene expression in the kidneys and adrenal glands of adult rats. Rats were kept on low (0.02%, w/w), normal (0.6%) or high (4%) NaC1 diets and plasma renin activity (PRA) and the relative abundance of renin messenger ribonucleic acid (mRNA) in renal and adrenal tissue were followed for 20 days. In animals on a normal-salt diet PRA and renal renin mRNA levels did not change with time. PRA values in animals on the low-salt diet increased transiently (about threefold) and then declined again during the third week of treatment. Renal renin mRNA levels in these animals paralleled the changes of PRA. Conversely, in the animals kept on a high-salt diet PRA values decreased transiently and renal renin mRNA decreased continuously to about 50% of control values. Arterial blood pressure measured in conscious animals was not significantly influenced by the different salt diets. To establish whether the changes in renin mRNA levels are mediated by renal nerve input, animals on the different diets were also studied after unilateral renal denervation. Renal nerve section led to a 50% decrease of renin mRNA levels in the denervated kidneys in animals kept on the normal-salt diet. In the animals on the lowsalt diet renin mRNA rose to similar levels in the denervated to those in the innervated kidney, while in animals receiving a high-salt diet renin mRNA was further decreased in the denervated kidneys. The abundance of renin mRNA in adrenal tissue was low and was estimated to be around 1% of that found in the kidneys. Adrenal renin mRNA levels also increased in animals kept on a low-salt diet and decreased in animals on high-salt diet. Taken together, our findings suggest that renin secretion and renin gene expression are inversely related to salt intake and that the influence of salt diet on these parameters has both transient and constant temporal components. Changes of blood pressure or nerve activity are not likely mediators of the effect of salt intake on renin expression. Since renal and adrenal renin mRNA levelsCorrespondence to: A. Kurtz change in parallel in response to alterations of salt intake we hypothesize the existence of a humoral factor that links renin expression to the rate of salt intake.

show abstract

Section: Renal Denervationmentioning

confidence: 99%

Influence of dietary NaCl intake on renin gene expression in the kidneys and adrenal glands of rats

et al. 1993

View full text Add to dashboard Cite

show abstract

“…Conversely, removal of the renal nerve influence by acute denervation of the kidney is associated with increased urine flow and sodium excretion (5,6). These phenomena have been attributed by many investigators to reflect a direct influence of renal nerves on the tubule reabsorption processes.…”

Section: Introductionmentioning

confidence: 99%

Role of renal sympathetic nerves in mediating hypoperfusion of renal cortical microcirculation in experimental congestive heart failure and acute extracellular fluid volume depletion.

Kon¹,

Yared²,

Ichikawa³

1985

J. Clin. Invest.

View full text Add to dashboard Cite

To evaluate the pathophysiologic importance of renal nerves in regulating the renal vasomotor tone, we measured several parameters of renal cortical microcirculation before and after acute renal denervation (DNx) in the following three groups of anesthetized Munich-Wistar rats: (group 1) congestive heart failure after surgically induced myocardial infarction (n = 10), (group 2) acute extracellular fluid volume depletion after deprivation of drinking water for 48 h (n = 8), and (group 3) sham or nontreated controls (n = 6). In the myocardial-infarcted rats, DNx led to a uniform increase in glomerular plasma flow rate of, on average, 36%. Single nephron glomerular filtration rate of myocardialinfarcted rats also increased despite a reduction in glomerular capillary hydraulic pressure. These changes were associated with a fall in arteriolar resistances, particularly in the efferent arteriole. The glomerular capillary ultrafiltration coefficient rose in all but one myocardial-infarcted animal. A similar hemodynamic pattern was seen after DNx in water-deprived animals. In every water-deprived animal, glomerular plasma flow rate and single nephron GFR increased on average by 28 and 14%, respectively. Again, afferent and efferent arteriolar resistances decreased significantly. Furthermore, the ultrafiltration coefficient increased uniformly and substantially with DNx.To ascertain the potential importance of the interaction between the renal nerves and angiotensin II in these circumstances, we compared the renal cortical hemodynamics in additional groups of water-deprived rats (group 4) after DNx (n = 15), (group 5) during inhibition of angiotensin II with saralasin (n = 15), and (group 6) during treatment with both saralasin and DNx (n = 15). No appreciable difference was detected between group 4 vs. 6. In contrast, substantial differences were noted between group 5 vs. 6: on average, the glomerular plasma flow rate was 26% higher and the afferent and efferent arteriolar resistances 25% and 27% lower, respectively, in group 6.Portions of these studies were presented at the annual meeting of the American Society of Nephrology, Washington, DC, 1984, and at the Annual Meeting of the Federations of American Societies for Experimental Biology, Washington, DC, 1985, and were published in abstract form (1984. Am. Soc. Nephrol. 17:134; and 1985. Clin. Res. 33:489A).Address correspondence to Dr. Kon, Laboratory of Renal Physiology.Receivedfor publication 8 January 1985 and in revisedform 12 July 1985.These observations provide direct evidence to indicate pathophysiologic importance of renal nerves in the profound intrarenal circulatory adjustments in prerenal circulatory impairment. The vasoconstrictive effects of renal nerves appear to be mediated in part by their stimulatory influence on angiotensin II release and their direct constrictor actions on pre-and postglomerular vessels as well.

show abstract

“…Bello-Reuss et al (6,19) reported that in anesthetized rats renal nerve stimulation produced an increase in sodium reabsorption which occurred in the proximal convoluted tubule in the absence of changes in GFR or renal blood flow and that renal denervation induced a marked increase in urinary sodium and water excretion independent of changes in GFR or renal blood flow. In contrast to the latter results, renal denervation per se did not alter urinary sodium and water excretion in SHRSP and renal sympathectomy increased GFR in SHRSP-AC.…”

Section: Discussionmentioning

confidence: 99%

“…This effect could be blocked by adrenolytic drugs (4,5). When renal denervation was produced in anesthetized rats by application of phenol to the renal artery, urinary sodium excretion increased approximately five to six-fold without changes in renal hemodynamics (6).…”

mentioning

confidence: 99%

Effects of renal sympathectomy on sodium and water excretion in stroke-prone spontaneously hypertensive rats.

Nagaoka

Kakihana

1982

Jpn.J.Pharmacol

View full text Add to dashboard Cite

Abstract-In stroke-prone spontaneously hypertensive rats (SHRSP), urinary excretion of sodium and water and glomerular filtration rate (GFR) are markedly decreased by acute normalization of the renal perfusion pressure using an aortic clamping technique.To examine the mechanism for the decreased sodium and water excretion, SHRSP rats were subjected to bilateral renal denervation.Sodium and water excretion and GFR in SHRSP with aortic clamping were significantly restored by the renal dener vation. The restoration was more prominent in the urine flow and GFR. When renal perfusion pressure was normalized by administration of sympa tholytic drugs in another group of SHRSP, sodium and water excretion were decreased.However, the extent of the decrease in urine flow but not in sodium excretion was significantly less than that in SHRSP with aortic constriction.GFR was not changed by administration of the sympatholytic drugs.Renal denervation lowered the blood pressure in SHRSP. These results suggest that renal sympathetic nerve activity is greatly involved in the reduced water excretion and partly involved in the reduced sodium excretion in SHRSP rats.

show abstract

Effects of acute unilateral renal denervation in the rat.

Cited by 215 publications

References 31 publications

Influence of dietary NaCl intake on renin gene expression in the kidneys and adrenal glands of rats

Influence of dietary NaCl intake on renin gene expression in the kidneys and adrenal glands of rats

Role of renal sympathetic nerves in mediating hypoperfusion of renal cortical microcirculation in experimental congestive heart failure and acute extracellular fluid volume depletion.

Effects of renal sympathectomy on sodium and water excretion in stroke-prone spontaneously hypertensive rats.

Contact Info

Product

Resources

About