Effects of adding an arbuscular mycorrhizal fungi inoculum and of distance to donor sites on plant species recolonization following topsoil removal

Abstract

Questions: Does addition of an arbuscular mycorrhizal fungi (AMF) inoculum increase the short-term restoration success of nutrient-poor grassland (NPG) after topsoil removal? Does distance to intact remnant grassland (IRG) patches affect restoration success, and does the effect of inoculum addition depend on distance to IRGs?Location: Meerdaal forest, Oud-Heverlee, Belgium.Methods: In a topsoil-removed site of 8.5 ha, where 24 IRG patches (ca. 10% of the area) were kept, 48 plots (1 m 2 ) were established at three distances (5, 10 and 20 m) from the edge of IRG patches. Half of the plots at each distance class were inoculated with a custom-made AMF inoculum, whereas the remaining plots were used as controls. We recorded plant species abundance in the plots, just before addition of the AMF inoculum and 1 yr after. We used repeated measures ANOVA to test for effects of inoculum addition, distance to IRG patches and their interaction on plant species richness, diversity and plant community similarity between IRG patches and plots. We also evaluated the response of AMF-dependent plant species, specialist species of NPG and species with specific seed dispersal adaptations.Results: Adding the inoculum positively affected species richness and/or diversity of all plant species, AMF-dependent species and specialist species. It increased plant community similarity to the IRG patches. Increasing distance from IRG patches had a negative effect on richness and/or diversity of all plant species and specialist species. The positive effect of inoculum addition on richness and/or diversity of all species, AMF-dependent species and specialist species decreased with increasing distance from IRG patches to the plots, likely indicating priority effects. Conclusions:The application of a custom-made AMF inoculum increased the short-term restoration success of NPG after topsoil removal. Dispersal limitation of specialist plant species of NPG, however, likely negatively affected the effect of inoculum addition. Apart from the AMF effect, the reported strongly positive short-time effect of inoculation was likely due to the high density of IRG patches at the site, and the presence of organic matter and other micro-organisms in the inoculum.

Initial soil community drives heathland fungal community trajectory over multiple years through altered plant–soil interactions

“…These plots were situated next to the inoculated plots and close to a larger area of abundant heathland vegetation, which poses a significant source of heathland taxa available to colonize them. It has been shown that the vicinity of source sites is an important factor promoting heathland community development (Torrez et al , ; van der Bij et al , ). Surprisingly though, despite the fact that control plots collectively contained the majority of plant and fungal taxa observed in other treatments, including heathland taxa, the increase in the strength of links between heathland plants and fungi was notably delayed or absent compared with the sod‐inoculated plots.…”

Fungal inoculants in the field: Is the reward greater than the risk?

“…for landscape restoration), or an unintended consequence. AMF inoculants have been shown to reduce plant biodiversity and/or prevent the establishment of native plants (Emam, 2016;Koziol, Bever, & Hawkes, 2015;Middleton et al, 2015;Torrez, Ceulemans, Mergeay, de Meester, & Honnay, 2016). Interestingly, inoculant provenance has been shown to be an important predictor of plant community response.…”

The role of plant mycorrhizal type and status in modulating the relationship between plant and arbuscular mycorrhizal fungal communities

“…Early in succession, effective AM fungal dispersal to and colonisation of sparsely vegetated sites may facilitate or 'drive' vegetation establishment (e.g. Torrez et al, 2016). Later in succession, when vegetation cover is closed and the availability of host plants is high, the mechanisms described by the passenger hypothesis may become more prevalent, that is, changes in plant community composition (e.g.…”