Effects of addition of ion-pair reagent to the mobile phase on electroosmotic flow velocity in pressurized planar electrochromatography

Hałka-Grysińska, Aneta; Płocharz, Paweł W.; Skwarek, Ewa; Janusz, W.; Dzido, Tadeusz H.

doi:10.1556/jpc.28.2015.2.8

Cited by 4 publications

(3 citation statements)

References 27 publications

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“…We discovered that eluents containing anionic or non-ionic surfactant (SDS, sodium cholate and Brij-35) generate considerably longer solute migration distances (SDS > Brij-35 > sodium cholate > CTAB > CTMA). Regarding anionic surfactants (SDS), literature data states that these have positive outcomes on the PPEC mobile phase flow 23 . This leads to longer solute migration distances, and the SDS-doped eluents separate all investigated f-moc derivatives quite well.…”

Section: Resultsmentioning

confidence: 99%

Separation of 9-Fluorenylmethyloxycarbonyl Amino Acid Derivatives in Micellar Systems of High-Performance Thin-Layer Chromatography and Pressurized Planar Electrochromatography

Polak

Traczuk

Misztal

2019

Sci Rep

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The problems with separation of amino acid mixtures in reversed-phase mode are the result of their hydrophilic nature. The derivatisation of the amino group of mentioned above solutes leads to their solution. For this purpose, 9-fluorenylmethoxycarbonyl chloroformate (f-moc-Cl) as the derivatisation reagent is often used. In our study, the separation of some f-moc- amino acid derivatives (alanine, phenylalanine, leucine, methionine, proline and tryptophan) with the use of micellar systems of reversed-phase high-performance thin-layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC) is investigated. The effect of surfactant concentration, its type (anionic, cationic and non-ionic) and mobile phase buffer pH on the discussed above solute migration distances are presented. Our work reveals that the increase of sodium dodecylsulphate concentration in the mobile phase has a different effect on solute retention in HPTLC and PPEC. Moreover, it also affects the order of solutes in both techniques. In PPEC, in contrast to the HPTLC technique, the mobile phase pH affects solute retention. The type of surfactant in the mobile phase also impacts solute retention and migration distances. A mobile phase containing SDS improves system efficiency in both techniques. Herein, such an effect is presented for the first time.

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Section: Resultsmentioning

confidence: 99%

Separation of 9-Fluorenylmethyloxycarbonyl Amino Acid Derivatives in Micellar Systems of High-Performance Thin-Layer Chromatography and Pressurized Planar Electrochromatography

Polak

Traczuk

Misztal

2019

Sci Rep

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“…However, the papers on separation of solutes with the use of IPC thin-layer chromatography (HPTLC) [6–9] or IPC capillary electrophoresis (CE) [10, 11] are also available. The effect of the ion-pairing agent additive to the mobile phase on the electroosmotic flow has also been investigated in pressurized planar electrochromatography (PPEC) [12].…”

Section: Introductionmentioning

confidence: 99%

“…Besides the electric field magnitude, also other variables prompt the solute migration in PPEC. Deteriorate of solute retention is the result of the mobile phase composition (buffer pH, its type and concentration, kind and amount of organic solvent, and chiral or ion-pairing additives) [12, 31–36], type of the stationary phase applied [32, 34, 37], and temperature in which the experiment is carried out [38]. One of the advantages of PPEC system is prewetting of an adsorbent layer with the mobile phase solution before separation process, and then the same eluent is used to feed adsorbent layer during PPEC experiment.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Phenolic Compound Separations by HPTLC and PPEC with SDS as the Mobile Phase Component

Polak

Traczuk

Kamińska

et al. 2019

Journal of Analytical Methods in Chemistry

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The application of the surfactant (sodium dodecyl sulphate, SDS) as the component of the water-organic mobile phase in thin-layer chromatography and pressurized planar electrochromatography is presented. The influence of various variables on the separation of various phenolic compounds (flavonoids and phenolic acids) as model compounds with systems containing surfactant is discussed. The effect of concentration of butanol and SDS as well as pH of the mobile phase buffer on migration distance of the solute zones is investigated. The presence of SDS in the eluent affects the butanol solubility in the mobile phase. It allows using higher organic solvent concentration systems compared with the mode without surfactant. The amount of SDS in the eluent has the effect on the solute retention, whereas the eluent buffer pH affects the migration distances of ionisable phenolic acids both in HPTLC and PPEC. The migration distances of flavonoid glycosides are considerably longer than those of pure flavonoids. Considering second group of investigated solutes, derivatives of the benzoic acid migrate longer distances in comparison with the cinnamic acid ones. In addition, in the majority of experiments, ionisable compounds (phenolic acids) migrate longer distances in PPEC than nonionisable compounds (flavonoids). Additionally, the order of solutes differs in the PPEC and HPTLC system.

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Particle-loaded monolithic materials for separations via planar electrochromatography

Gruber‐Wölfler

Braunbruck

Laskowski

et al. 2016

JPC - Journal of Planar Chromatography - Modern TLC

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Effects of addition of ion-pair reagent to the mobile phase on electroosmotic flow velocity in pressurized planar electrochromatography

Cited by 4 publications

References 27 publications

Separation of 9-Fluorenylmethyloxycarbonyl Amino Acid Derivatives in Micellar Systems of High-Performance Thin-Layer Chromatography and Pressurized Planar Electrochromatography

Separation of 9-Fluorenylmethyloxycarbonyl Amino Acid Derivatives in Micellar Systems of High-Performance Thin-Layer Chromatography and Pressurized Planar Electrochromatography

Comparison of Phenolic Compound Separations by HPTLC and PPEC with SDS as the Mobile Phase Component

Particle-loaded monolithic materials for separations via planar electrochromatography

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