Effects of Addition of Sodium Lauryl Sulfate on Frozen-thawed Canine Spermatozoa

Hori, Tatsuya; H, Kaseki; Fukuhara, Youko; Oba, Hiromichi; Mizutani, Tatsuji; Kawakami, Eiichi; Tsutsui, Toshihiko

doi:10.1292/jvms.68.1125

Cited by 7 publications

(6 citation statements)

References 17 publications

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“…Furthermore, several studies have reported the usefulness of frozen semen extender containing SLS in several animals (the cow [3], mouse [7], and goat [1,2]). We have also reported previously that addition of 2 mg/ml of SLS to dog semen for freezing led to results similar to those obtained by addition of 0.75% OEP [8]. However, no studies have reported addition of SLS to cat semen for freezing.…”

supporting

confidence: 71%

Usefulness of Addition of Orvus ES Paste and Sodium Lauryl Sulfate to Frozen Feline Semen

Mizutani

Sumigama²,

Nagakubo

et al. 2010

The Journal of Veterinary Medical Science

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ABSTRACT. It has been shown that addition of the surfactant Orvus ES paste (OEP) and its main component sodium lauryl sulfate (SLS) to boar or dog semen before freezing improves post-thaw sperm motility and protects acrosome caps. In this study, we investigated the usefulness of the addition of OEP (0, 1, 2 and 4%) or SLS (0, 1, 2, 3 and 4 mg/ml) to cat ejaculates before freezing and their concentrations. Among the OEP addition groups, the 1% OEP group showed higher sperm motility than the other groups. Among the SLS addition groups, the 3 mg/ml SLS group showed slightly higher sperm motility and viability than the other groups. Comparison between the 1% OEP and 3 mg/ml SLS addition groups suggested a higher percentage of sperm with an acrosome cap in the 1% OEP group. The other sperm properties did not significantly differ between the 2 groups. These results indicate that addition of 1% OEP or 3 mg/ ml SLS is effective for freezing of cat ejaculated semen.KEY WORDS: acrosome cap, cat, frozen semen, orvus es paste, sodium lauryl sulfate.J. Vet. Med. Sci. 72(1): 23-27, 2010 In regard to artificial insemination (AI) with frozen feline semen, intravaginal [6,11,19] and intrauterine [6,18,19] inseminations with ejaculates have each been reported in three studies, and intravaginal [20] and intrauterine [15,20] inseminations with epididymal sperm have each been reported in one and two studies, respectively. In our 2003 year study using epididymal sperm [15], we added 7% (v/v) glycerol and 1% Orvus ES paste (OEP, also known as Equex STM paste, Nova Chemical Sales, Inc., Scituate, MA, U.S.A.) to semen as cryoprotective agents and achieved a conception rate of 27.3%. However, we did not evaluate the usefulness of addition of OEP for cryopreservation of feline semen or its optimal concentration. Addition of OEP along with glycerol for the cryopreservation of boar [12] and dog [16,17] semen has been shown to protect the acrosome caps of sperm, thereby increasing and maintaining post-thaw sperm motility for a long period of time. In 2004, Axnér et al. [5] investigated the usefulness of Equex STM paste for cryopreservation of feline epididymal sperm and showed that addition 0.5% (v/v) Equex STM paste led to greater protection of the acrosome caps of sperm after freeze-thawing than no addition, but sperm motility was lower (sperm survival was shorter) 4-6 hr after thawing than in the case of no addition. Our search of the literature revealed no studies in which addition of OEP for semen cryopreservation adversely affected post-thaw sperm motility. It remains unclear whether the results of the study by Axnér et al. [5] regarding cryopreservation of epididymal sperm apply to cryopreservation of cat ejaculates.OEP is known to contain mainly sodium lauryl sulfate (SLS), but data on its concentration and the remaining constituents have not been published. It has been suggested that the effect of SLS might be exerted by modifying the structure of egg yolk lipoproteins in the extracellular medium [4]. Kato et al. [9] employed ...

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supporting

confidence: 71%

Usefulness of Addition of Orvus ES Paste and Sodium Lauryl Sulfate to Frozen Feline Semen

Mizutani

Sumigama²,

Nagakubo

et al. 2010

The Journal of Veterinary Medical Science

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“…In 2009, we investigated the effectiveness and optimal concentration of OEP and its main ingredient, SLS, to cryopreserve cat semen, and found that the addition of OEP or SLS for the cryopreservation of cat semen clearly improved the post-thaw semen quality, compared with no addition, and that the optimal concentrations of OEP and SLS were 1.0% and 3 mg/ml, respectively [9]. The effectiveness of OEP and its main ingredient of SLS as a semen cryoprotectant has also been shown in dogs and pigs [7,8].In this study, we performed IUI with semen that had been cryoprotected by the addition of glycerol and 1% OEP or 3 mg/ml of SLS, and compared its fertilizing ability.Animals: Mixed breed cats bred and maintained in our colony were used. Five male cats aged 2-11 years [mean: 5.6 ± 1.6 years (SE)] and weighing 4.3-5.0 kg (mean: 4.6 ± 0.1 kg) with a normal semen quality, copulation capability, and fertility were used.…”

mentioning

confidence: 93%

Surgical Intrauterine Insemination with Cat Semen Cryopreserved with Orvus ES Paste or Sodium Lauryl Sulfate

Tsutsui

Mizutani

Matsubara

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. The mean post-thaw sperm motilities of feline frozen semen prepared with 1% OEP or 3 g/ml SLS as a cryoprotective agent, in addition to 7% glycerin, were 35.0 2.4 and 37.0 2.5%, respectively, showing no significant difference. On unilateral intrauterine insemination (UIUI) using these semen samples at a sperm number of 40  10 6 , the conception rate was 70.0% (7/10) in the OEP group and 30% (3/10) in the SLS group, showing that the rate was higher in the OEP group, but the difference was not significant. It was suggested that sperm in frozen semen showing the above qualities were transferred to the contralateral uterine horn on UIUI.KEY WORDS: feline, frozen semen, OEP, sodium lauryl sulfate.J. Vet. Med. Sci. 73(2): 259-262, 2011 Domestic cats have been used as models of rare wildcats in studies of various artificial breeding techniques, among which artificial insemination (AI) techniques, particularly using frozen semen, are the most important.Reports on intravaginal insemination (IVI) [11], intrauterine insemination (IUI) [15], and IVI and IUI [5,17] with frozen cat ejaculates have been published. In addition, one study addressing IUI with epididymal sperm has been published [16]. In these studies, glycerol alone or in combination with Orvus ES paste OEP, also known as Equex STM paste [Nova Chemical Sales, Inc., Situate MA, U.S.A.], a surfactant, was used as a cryoprotectant. OEP has become important to protect sperm acrosomes in the cryopreservation of pig [8] and dog [13] semen. Although the main ingredient of OEP is known to be sodium lauryl sulfate (SLS), data on its concentration and the remaining ingredients have not been published. It has been suggested that the effect of SLS involves the modification of the structure of egg yolk lipoproteins in the extracellular medium. We first used OEP as a cryoprotectant to more effectively freeze cat sperm [16]. Later, Axner et al. [4], Chatdarong et al. [5] and Villaverde et al. [17] reported the use of Equex STM paste to cryopreserve cat semen. However, they did not determine the optimal concentration of OEP to be added as a cryoprotectant to freeze cat semen. In 2009, we investigated the effectiveness and optimal concentration of OEP and its main ingredient, SLS, to cryopreserve cat semen, and found that the addition of OEP or SLS for the cryopreservation of cat semen clearly improved the post-thaw semen quality, compared with no addition, and that the optimal concentrations of OEP and SLS were 1.0% and 3 mg/ml, respectively [9]. The effectiveness of OEP and its main ingredient of SLS as a semen cryoprotectant has also been shown in dogs and pigs [7,8].In this study, we performed IUI with semen that had been cryoprotected by the addition of glycerol and 1% OEP or 3 mg/ml of SLS, and compared its fertilizing ability.Animals: Mixed breed cats bred and maintained in our colony were used. Five male cats aged 2-11 years [mean: 5.6 ± 1.6 years (SE)] and weighing 4.3-5.0 kg (mean: 4.6 ± 0.1 kg) with a normal semen quality, copulation capability, and ...

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“…The addition of detergents such as Equex STM paste, Orvus ES paste, and sodium dodecyl sulfate (SDS) to egg yolk-supplemented extenders appears to have beneficial effects on spermatozoa motility after thawing [ 27 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 ]. Although SDS is believed to have effects on membrane fluidity upon interaction with the egg yolk [ 54 ], there are relatively few litters following artificial insemination using the same ( Table 1 ).…”

Section: Extender For Canine Spermatozoamentioning

confidence: 99%

Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs

2022

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Assisted reproductive techniques (ARTs), such as artificial insemination, in vitro fertilization, and cryopreservation of gametes/zygotes, have been developed to improve breeding and reproduction of livestock and for the treatment of human infertility. Their widespread use has contributed to improvements in human health and welfare. However, in dogs, only artificial insemination using frozen semen is readily available as an ART to improve breeding and control genetic diversity. A recent priority in sperm cryopreservation is the development of alternatives to egg yolk, which is widely used as a component of the sperm extender. Egg yolk can vary in composition among batches and is prone to contamination by animal pathogens. The latter can be a problem for international exchange of cryopreserved semen. Low-density lipoprotein and skim milk are promising candidates for use as extenders, to ensure fertility after artificial insemination. Although not tested for its effects on fertility following artificial insemination, polyvinyl alcohol may also be a useful alternative to egg yolk as an extender. The development of cryopreservation techniques for canine embryos lags behind that for other mammals, including humans. However, given the success of non-surgical embryo transfer in 2011, studies have sought to refine this approach for practical use. Research on sperm cryopreservation has yielded satisfactory results. However, investigation of other approaches, such as cryopreservation of oocytes and gonadal tissues, remains insufficient. Techniques for the efficient induction of estrus may aid in the development of successful canine ARTs.

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Effects of Addition of Sodium Lauryl Sulfate on Frozen-thawed Canine Spermatozoa

Cited by 7 publications

References 17 publications

Usefulness of Addition of Orvus ES Paste and Sodium Lauryl Sulfate to Frozen Feline Semen

Usefulness of Addition of Orvus ES Paste and Sodium Lauryl Sulfate to Frozen Feline Semen

Surgical Intrauterine Insemination with Cat Semen Cryopreserved with Orvus ES Paste or Sodium Lauryl Sulfate

Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs

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