Effects of Adipose Tissue-Derived Stem Cell Therapy After Myocardial Infarction: Impact of the Route of Administration

Rigol, Montserrat; Solanes, Núria; Farré, Jordi; Roura, Santiago; Roqué, Mercè; Berruezo, Antonio; Bellera, Neus; Novensá, Laura; Tamborero, David; Prat‐Vidal, Cristina; Huzman, MA Ángeles; Batlle, Montserrat; Hoefsloot, Margo; Sitges, Marta; Ramírez, José; Dantas, Ana Paula; Merino, Anna; Sanz, Ginés; Brugada, Josep; Bayés‐Genís, Antoni; Heras, Magda

doi:10.1016/j.cardfail.2009.12.006

Cited by 72 publications

(65 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Adipose tissue-derived mesenchymal stem cells (ATMSCs) are available in large amounts, expand easily and rapidly in vitro and can differentiate into multiple tissue lineages [2,3]. Furthermore, experimental studies have demonstrated in small animal models that engrafted ATMSCs expressed endothelial and cardiac markers [4,5], and our group has shown in a porcine model of AMI that engrafted autologous ATMSCs were incorporated into newly formed vessels and enhanced neovascularisation [6]. 1 Both authors have contributed equally to this study.…”

Section: Introductionmentioning

confidence: 99%

“…Cells were expanded and kept at À80°C until further use. Prior to in vivo administration, frozen aliquots were thawed and some of these ATMSCs (2 9 10 6 cells) were labelled by transfection with a gene encoding b-Gal [6] ( Figure S1 and Data S1). One inguinal lymph node was excised to determine donor-specific antibodies formation in sera of recipient animals.…”

Section: Adipose Tissue Collection and Cell Labellingmentioning

confidence: 99%

“…Donor animals (n = 6) were anesthetised ( Figure S1 and Data S1), and subcutaneous adipose tissue was acquired to isolate and characterize mesenchymal stem cells as described previously [6]. Cells were expanded and kept at À80°C until further use.…”

Section: Adipose Tissue Collection and Cell Labellingmentioning

confidence: 99%

“…Recipient animals were anaesthetized as previously described [6], and cardiac function was assessed by intracardiac echocardiogram (AcuNav Catheter, Biosense, Diamond Bar, CA, USA). Left ventricular end-diastolic (LVEDV) and systolic (LVESV) volumes and ejection fraction (LVEF) were measured using the Simpson method.…”

Section: Ami Proceduresmentioning

confidence: 99%

See 3 more Smart Citations

Allogeneic adipose stem cell therapy in acute myocardial infarction

Rigol

Solanes

Roura

et al. 2013

Eur J Clin Investigation

Self Cite

View full text Add to dashboard Cite

Background Stem cell therapy offers a promising approach to reduce the long-term mortality rate associated with heart failure after acute myocardial infarction (AMI). To date, in vivo translational studies have not yet fully studied the immune response to allogeneic adipose tissue-derived mesenchymal stem cells (ATMSCs). We analysed the immune response and the histological and functional effects of allogeneic ATMSCs in a porcine model of reperfused AMI and determine the effect of administration timing.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Adipose Tissue Collection and Cell Labellingmentioning

confidence: 99%

Section: Adipose Tissue Collection and Cell Labellingmentioning

confidence: 99%

Section: Ami Proceduresmentioning

confidence: 99%

See 2 more Smart Citations

Allogeneic adipose stem cell therapy in acute myocardial infarction

Rigol

Solanes

Roura

et al. 2013

Eur J Clin Investigation

Self Cite

View full text Add to dashboard Cite

show abstract

“…[10] As an alternative to systemic use, local application of ADSC to diseased tissue can improve the efficiency of implantation. [12] It avoids the dependence of chemokines secreted by diseased tissues and the risk of systemic use. At present, some diseases have been successfully treated by local application of ADSC, such as myocardial necrosis after myocardial infarction, bone defect and local wound.…”

Section: Usage Modementioning

confidence: 99%

Advances in the research of basic study and clinical application of adipose-derived mesenchymal stem cells

Sheng-jun

Wang

et al. 2018

DCC

View full text Add to dashboard Cite

Since the discovery of adipose-derived mesenchymal stem cell (ADSC) in more than ten years, a great progress has been made from its basic research to clinical application. Compared with bone marrow mesenchymal stem cells, ADSC is more abundant in reserve, easier to obtain with fewer injuries and less complications. These cells have multiple differentiation potential and can differentiate into adipocytes, chondrocytes and osteoblasts with the influence of different inducing factors. Early studies of ADSC mainly focused on the ability of multi-directional differentiation, especially on the regeneration of bone defects and cartilage tissue. At present, the researches mainly focus on immunoregulation and paracrine function of ADSC. Although ADSC has made a great progress in clinical application, the cell preparation, use pattern, and mechanisms in clinical treatment are not clear. This paper elaborates on these issues.

show abstract

Intracoronary and retrograde coronary venous myocardial delivery of adipose‐derived stem cells in swine infarction lead to transient myocardial trapping with predominant pulmonary redistribution

Hong

Hou

Brinton

et al. 2013

Cathet Cardio Intervent

View full text Add to dashboard Cite

Objectives To examine the comparative fate of adipose-derived stem cells (ASCs) as well as their impact on coronary microcirculation following either retrograde coronary venous or arterial delivery. Background Local delivery of ASCs to the heart has been proposed as a practical approach to limiting the extent of myocardial infarction. Mouse models of mesenchymal stem cell effects on the heart have also demonstrated significant benefits from systemic (intravenous) delivery, prompting a question about the advantage of local delivery. There has been no study addressing the extent of myocardial vs. systemic disposition of ASCs in large animal models following local delivery to the myocardium. Methods In an initial experiment, dose-dependent effects of ASC delivery on coronary circulation in normal swine were evaluated to establish a tolerable ASC dosing range for intracoronary delivery. In a set of subsequent experiments, an anterior acute myocardial infarction (AMI) was created by balloon occlusion of the proximal left anterior descending (LAD) artery, followed by either intracoronary (IC) or retrograde coronary venous (RCV) infusion of 107 111Indium-labeled autologous ASCs 6 days following AMI. Indices of microcirculatory resistance (IMR) and coronary flow reserve (CFR) were measured before sacrifices to collect tissues for analysis at 1 or 24 hours after cell delivery. Results IC delivery of porcine ASCs to normal myocardium was well-tolerated up to a cumulative dose of 14×106 cells (approximately 0.5×106 cells/kg). There was evidence suggesting microcirculatory trapping of ASC: at unit doses of 50×106 ASCs, IMR and CFR were found to be persistently altered in the target LAD distribution at 7 days following delivery, while at 10×106 ASCs, only CFR was altered. In the context of recent MI, a significantly higher percentage of ASCs was retained at 1 hour with IC delivery compared to RCV delivery (57.2 ± 12.7% vs. 17.9 ± 1.6%, p=0.037) but this initial difference was not apparent at 24 hours (22.6 ± 5.5% vs. 18.7 ± 8.6%; p= 0.722). In both approaches, most ASC redistributed to the pulmonary circulation by 24 hours post-delivery. There were no significant differences in CFR or IMR following ASC delivery to infarcted tissue by either route. Conclusions Selective intravascular delivery of ASC by coronary arterial and venous routes leads to similarly limited myocardial cell retention with predominant redistribution of cells to the lungs. Intracoronary arterial delivery of ASC leads to only transiently greater myocardial retention, which is accompanied by obstruction of normal regions of coronary microcirculation at higher doses. The predominant intrapulmonary localization of cells following local delivery via both methods prompts the notion that systemic delivery of ASC might provide similarly beneficial outcomes while avoiding risks of inadvertent microcirculatory compromise.

show abstract

Effects of Adipose Tissue-Derived Stem Cell Therapy After Myocardial Infarction: Impact of the Route of Administration

Cited by 72 publications

References 33 publications

Allogeneic adipose stem cell therapy in acute myocardial infarction

Allogeneic adipose stem cell therapy in acute myocardial infarction

Advances in the research of basic study and clinical application of adipose-derived mesenchymal stem cells

Intracoronary and retrograde coronary venous myocardial delivery of adipose‐derived stem cells in swine infarction lead to transient myocardial trapping with predominant pulmonary redistribution

Contact Info

Product

Resources

About