Effects of Adjuvants on Safety and Efficacy of an Escherichia coli J5 Bacterin

Hogan, J.S.; Cannon, V.B.; Smith, Kenneth L.; Rinehart, Carol L.; Miller, Shannon C.

doi:10.3168/jds.s0022-0302(05)72715-6

Cited by 9 publications

(10 citation statements)

References 14 publications

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“…The results of the vaccinated group were compared with those of control by using T-test, it was noticed that the mean OD of the vaccinated groups increased significantly than those of control groups. Same results were obtained by Hogan et al (2005), Pellegrino et al (2008) and Perez et al (2009). As shown in Table (4) and Fig.…”

Section: Discussionsupporting

confidence: 85%

Efficacy of a locally prepared bovine mastitis vaccine

Sayed

W.S.

Ahmed

et al. 2015

Benha Veterinary Medical Journal

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A B S T R A C TThe objective of the present work is to prepare and evaluate the effectiveness of a locally prepared polyvalent vaccine against mastitis from the most common causes of mastitis. Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli were the most prevalent bacteria recovered from clinical and subclinical mastitis. Montanide ISA-206 adjuvanted inactivated polyvalent vaccine containing the three strains was prepared. Twenty pregnant cows were inoculated intramuscularly with the prepared polyvalent vaccine two months prior to calving and boostering at day 21 from the primary injection. Serum samples from vaccinated and non-vaccinated cows were collected at the 1 st , 3 rd , 8 th , 12 th , 16 th , 20 th and 24 th weeks post vaccination and evaluated immunologically using ELISA. The results showed that immune response was significantly higher in the vaccinated group than that of controls. These results could be indicated the safety and effectiveness of the vaccine in reduction of incidence and severity of clinical cases of mastitis but further studies should be done to elucidate the possibility of field application and effectively.

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Section: Discussionsupporting

confidence: 85%

Efficacy of a locally prepared bovine mastitis vaccine

Sayed

W.S.

Ahmed

et al. 2015

Benha Veterinary Medical Journal

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“…We have developed a collection of E . coli isolates that can be divided into two distinct groups: those isolated from intramammary infections (IMI) displaying transient infection characteristics (see for examples [ 14 , 15 ]) and those isolated from IMI associated with persistently infected mammary glands (see for examples [ 16 ]). The purpose of this study is to use genome sequence data derived from both transient and persistent MPEC isolates to construct a genome-based phylogeny that places MPEC in its phylogenetic context with other E .…”

Section: Introductionmentioning

confidence: 99%

Genome Based Phylogeny and Comparative Genomic Analysis of Intra-Mammary Pathogenic Escherichia coli

et al. 2015

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Escherichia coli is an important cause of bovine mastitis and can cause both severe inflammation with a short-term transient infection, as well as less severe, but more chronic inflammation and infection persistence. E. coli is a highly diverse organism that has been classified into a number of different pathotypes or pathovars, and mammary pathogenic E. coli (MPEC) has been proposed as a new such pathotype. The purpose of this study was to use genome sequence data derived from both transient and persistent MPEC isolates (two isolates of each phenotype) to construct a genome-based phylogeny that places MPEC in its phylogenetic context with other E. coli pathovars. A subsidiary goal was to conduct comparative genomic analyses of these MPEC isolates with other E. coli pathovars to provide a preliminary perspective on loci that might be correlated with the MPEC phenotype. Both concatenated and consensus tree phylogenies did not support MPEC monophyly or the monophyly of either transient or persistent phenotypes. Three of the MPEC isolates (ECA-727, ECC-Z, and ECA-O157) originated from within the predominately commensal clade of E. coli, referred to as phylogroup A. The fourth MPEC isolate, of the persistent phenotype (ECC-1470), was sister group to an isolate of ETEC, falling within the E. coli B1 clade. This suggests that the MPEC phenotype has arisen on numerous independent occasions and that this has often, although not invariably, occurred from commensal ancestry. Examination of the genes present in the MPEC strains relative to the commensal strains identified a consistent presence of the type VI secretion system (T6SS) in the MPEC strains, with only occasional representation in commensal strains, suggesting that T6SS may be associated with MPEC pathogenesis and/or as an inter-bacterial competitive attribute and therefore could represent a useful target to explore for the development of MPEC specific inhibitors.

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“…The more successful vaccination protocols for mastitis control have been with the J5 E. coli bacterin vaccine which is administered initially either just before or at the time of drying off [87,91,92,93,94,95,96]. These typically are followed by additional vaccine doses approximately mid-dry period.…”

Section: Immune Milkmentioning

confidence: 99%

Perspectives on Immunoglobulins in Colostrum and Milk

2011

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Immunoglobulins form an important component of the immunological activity found in milk and colostrum. They are central to the immunological link that occurs when the mother transfers passive immunity to the offspring. The mechanism of transfer varies among mammalian species. Cattle provide a readily available immune rich colostrum and milk in large quantities, making those secretions important potential sources of immune products that may benefit humans. Immune milk is a term used to describe a range of products of the bovine mammary gland that have been tested against several human diseases. The use of colostrum or milk as a source of immunoglobulins, whether intended for the neonate of the species producing the secretion or for a different species, can be viewed in the context of the types of immunoglobulins in the secretion, the mechanisms by which the immunoglobulins are secreted, and the mechanisms by which the neonate or adult consuming the milk then gains immunological benefit. The stability of immunoglobulins as they undergo processing in the milk, or undergo digestion in the intestine, is an additional consideration for evaluating the value of milk immunoglobulins. This review summarizes the fundamental knowledge of immunoglobulins found in colostrum, milk, and immune milk.

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Effects of Adjuvants on Safety and Efficacy of an Escherichia coli J5 Bacterin

Cited by 9 publications

References 14 publications

Efficacy of a locally prepared bovine mastitis vaccine

Efficacy of a locally prepared bovine mastitis vaccine

Genome Based Phylogeny and Comparative Genomic Analysis of Intra-Mammary Pathogenic Escherichia coli

Perspectives on Immunoglobulins in Colostrum and Milk

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