Effects of administration of oxytocin on embryonic survival in progestogen supplemented cattle

LeMaster, Jeanette; Seals, R. C.; Hopkins, Fred M.; Schrick, F. N.

doi:10.1016/s0090-6980(99)00007-6

Cited by 41 publications

(35 citation statements)

References 38 publications

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“…Other evidence obtained in these studies supports the concept that luteal oxytocin and luteal and uterine PGF 2␣ are key components of the embryotoxic effect as proposed by Lemaster et al [44]. The expression of genes necessary for prostaglandin synthesis increased following multiple injections of PGF 2␣ beginning on Day 4 in the experiments reported here and increased luteal production of prostaglandins has been observed following treatment with PGF 2␣ [45] or of luteal cells with oxytocin in vitro [46].…”

Section: Discussionsupporting

confidence: 90%

“…However, the combination of removal of the CL and treatment with the prostaglandin inhibitor restored pregnancy rates when progesterone supplementation was provided [3]. Treatment of cows with oxytocin on Days 4-8 after estrus reduced pregnancy rates to a degree similar to rates seen in postpartum cows or cyclic cows treated with PGF 2␣ and given supplemental progestogen [44]. Concurrent treatment with a PGHS inhibitor overcame the effects of oxytocin on pregnancy rates.…”

Section: Discussionmentioning

confidence: 98%

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Increased Expression of Insulin-Like Growth Factor Binding Protein-1 During Induced Regression of Bovine Corpora Lutea1

Sayre

Taft

Inskeep

et al. 2000

Biology of Reproduction

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Three experiments were conducted to examine gene expression during induced luteal regression in the cow; the initial purpose was the identification of potential embryotoxins. In experiment 1, changes in gene expression in the corpus luteum (CL) were identified by differential display reverse transcription-polymerase chain reaction (DD-PCR) during the first 72 h of luteal regression in cows treated with prostaglandin F(2alpha) (PGF(2alpha)) on Days 4-7 after estrus. Expression of insulin-like growth factor-binding protein-1 (IGFBP-1) was up-regulated, with greatest expression at 24 h (P < 0.05) after treatment with PGF(2alpha) began. In experiment. 2, IGFBP-1 and its mRNA were quantified in CL collected 24 or 48 h after treatment with PGF(2alpha) on Day 4 or 10 after estrus. Because local mechanisms for exchange of hormones between the ovary and uterus are known in ruminants, uterine flushings were assayed for IGFBP-1 to seek evidence of local transfer of luteal IGFBP-1 to the uterus. IGFBP-1 mRNA was increased (P < 0.05) in CL 24 h after treatment when PGF(2alpha) that began on Day 10, and by 48 h after treatment that began on Day 4. Concentrations of IGFBP-1 increased (P < 0.05) in a pattern similar to mRNA, by 24 h on Day 10, and by 48 h on Day 4. Concentrations of IGFBP-1 in uterine flushings did not change on either day. Concentrations of progesterone decreased (P < 0.05) by 8 h after treatment with PGF(2alpha) that began on Day 10, but not until 24 h after treatment that began on Day 4. In experiment 3, cows received either saline or PGF(2alpha) and CL were collected 2 or 10 h after a single treatment, or 2 h after a second treatment that was given 8 h after the first. Expression of IGFBP-1 was increased by 2 h after treatment with PGF(2alpha) on both Days 4 and 10 after estrus. In conclusion, secretion of IGFBP-1 is increased during luteolysis, and may inhibit the steroidogenic effects of insulin-like growth factor-I (IGF-I), but no evidence was found to implicate IGFBP-1 in the embryotoxic effect of regressing CL.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 98%

Increased Expression of Insulin-Like Growth Factor Binding Protein-1 During Induced Regression of Bovine Corpora Lutea1

Sayre

Taft

Inskeep

et al. 2000

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…It has been demonstrated that oxytocin stimulates release of arachidonic acid and prostaglandin F 2a from decidual cells (16). Administration of oxytocin that stimulated uterine PGF 2a expression was shown to decrease both endometrial blood supply and embryonic survival in cattle (17). Oxytocin receptor blockade, apart from the reduction of uterine contractions, is reported to inhibit the stimulation of uterine production of prostaglandins (18).…”

Section: Discussionmentioning

confidence: 99%

Oxytocin antagonists may improve infertility treatment

Pierzyński

Reinheimer

Kuczyński

2007

Fertility and Sterility

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“…In previous work [5], PGF added to the development medium decreased the percentage of bovine embryos reaching the blastocyst stage. On day 7 bovine blastocysts expressed the COX2 gene and TNFα expression was lower in embryos that went on to produce a pregnancy [11] Furthermore, treatment with flunixin meglumine, another prostaglandin synthesis inhibitor, blocked the detrimental effects of oxytocin administration on bovine embryo [12] and addition of a prostaglandin receptor antagonist improved bovine embryo survival [13]. Taken together, these observations support the suggestion that PGs mediate a detrimental effect on blastocyst development.…”

Section: Discussionmentioning

confidence: 82%

Tumor necrosis factor alpha inhibits in vitro bovine embryo development through a prostaglandin mediated mechanism

Jackson

Farin

Whisnant

2012

J Animal Sci Biotechnol

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Mastitis or other infectious diseases have been related to reduced fertility in cattle. Inflammatory cytokines such as tumor necrosis factor α (TNFα) are released in response to infection and may have negative effects on embryo development. In the current study the effect of exposure to TNFα on the development of in vitro fertilized bovine embryos was examined. Indomethacin, a prostaglandin synthesis inhibitor, was used to determine if blockade of prostaglandin synthesis would alter the effects of TNFα. Ovaries were obtained from a local abattoir and immature COC were isolated from 2-10 mm follicles, in vitro matured and fertilized. After fertilization, groups of presumptive zygotes were randomly placed into either control development medium, medium containing 25 ng/mL TNFα or medium containing 25 ng/mL TNFα plus 1 μg/mL indomethacin. The proportion of blastocysts formed was assessed at day 7 of culture. Fewer embryos exposed to TNFα alone reached the blastocyst stage (17.5 ± 2.4%, P < 0.01) compared with controls (30.5 ± 2.4%) or embryos developed in TNFα plus indomethacin (25.8 ± 2.8%). There was no difference between control embryos and embryos developed in TNFα plus indomethacin. These results indicate that TNFα is inhibitory to the in vitro development of bovine embryos and that this inhibition may be mediated by prostaglandins because it can be blocked by indomethacin.

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Effects of administration of oxytocin on embryonic survival in progestogen supplemented cattle

Cited by 41 publications

References 38 publications

Increased Expression of Insulin-Like Growth Factor Binding Protein-1 During Induced Regression of Bovine Corpora Lutea1

Increased Expression of Insulin-Like Growth Factor Binding Protein-1 During Induced Regression of Bovine Corpora Lutea1

Oxytocin antagonists may improve infertility treatment

Tumor necrosis factor alpha inhibits in vitro bovine embryo development through a prostaglandin mediated mechanism

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