Effects of adrenergic agents on glycoprotein secretion from acini and granular convoluted tubules of the rat submandibular gland.

Differential secretion of proteins by rat submandibular acini and granular ducts on graded autonomic nerve stimulations.

“…Interestingly, only peroxidase, and not kallikrein or tonin, concentration and output were increased at the lower sympathetic stimulation frequencies (0 1-2 Hz). Thus, our findings also serve to confirm Rigby & Templeton's (1984) (Kelly, 1985 (Matthews, 1974;Orstavik & Gautvik, 1977;Abe & Dawes, 1978;Iwabuchi et al 1985); secretory responses are predominantly fl-adrenergic for the acini and a-adrenergic for the granular ducts. However, this cannot explain the requirement for different impulse frequencies in order to activate acinar and ductal cells.…”

Effects of dobutamine and terbutaline on the secretion of glycoproteins from the acinar cells of the rat submandibular gland.

“…We found that characteristic glycoproteins in the acini are secreted into the saliva after stimulation by the non-specific 1 -adrenergic agent isoproterenol (15), as well as after stimulation by the cholinergic agent pilocar pine (16) and by substance P (17), whereas glycoproteins characteristic of the granular convoluted tubules are secreted in response to the a,-adrenergic agent, methoxamine (18,19).…”

Effects of Substance P on Glycoprotein Secretion from Acinar Cells of the Rat Submandibular Gland

“…In addition, substance P was proposed to act on acinar and duct cells of the rat submandibular gland by analysis of elec trolytes in the peptide-evoked saliva (6). We reported previously that glycoprotein species markedly differ between acini and granular convoluted tubules of the rat submandibular gland (7) and that character istic glycoprotein species in acini are secreted into the saliva by 8-adrenergic and cholinergic agonists, whereas those in granular con voluted tubules are secreted by a-adrenergic agonists (8)(9)(10). The present study was carried out to elucidate the action site of substance P on the rat submandibular gland by comparing electrophoretic profiles of glycoprotein species in isolated functional segments of the gland with those in substance P-evoked saliva.…”

“…Methods for col lecting submandibular saliva and for micro dissecting parenchymal components were the same as reported previously (7)(8)(9)(10). Briefly, rats were anesthetized with urethane (1.5 g/kg).…”

“…As reported previously, there is an electro phoretic difference in glycoprotein species between acini and granular convoluted tubules of the rat submandibular gland (7). Accordingly, it is possible to determine the action site of various autonomic agents on the functional segments of the gland by comparing electrophoretic patterns of glycoprotein species in evoked saliva with those of acini or granular convoluted tubules (8)(9)(10).…”