Effects of adriamycin, an anticancer drug showing testicular toxicity, on fertility in male rats.

Imahie, Hiroshi; Adachi, Tamiko; Nakagawa, Yoko; Nagasaki, T.; Yamamura, Takaaki; Hori, Masaki

doi:10.2131/jts.20.183

Cited by 40 publications

(19 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our data also showed that sperm production, morphology and motility were decreased dosedependently in the doxorubicin-treated groups. This observation was consistent with previous studies using mice (Oshio et al, 1987) and rats (Imahie et al, 1995;Prahalathan et al, 2005). A shedding of immature germ cells, indicating the disruption of sperm differentiation, and a decrease in the number of Sertoli cells were also observed in the treated groups.…”

Section: Discussionsupporting

confidence: 93%

Evaluation of testicular toxicology of doxorubicin based on microarray analysis of testicular specific gene expression

et al. 2011

View full text Add to dashboard Cite

Toxicity of chemical substances to male reproductive system has been focused since the effects of a pesticide, dibromochloropropane (DBCP), on human spermatogenesis were discovered (Whorton et al., 1977). Because the damages on reproductive system may cause infertility and endanger the survival of the species, it is necessary to establish a simple way to evaluate the mechanism of reproductive toxicity of chemical substances for controlling and preventing their adverse effects. Testicular toxicity, a major male reproductive toxicity (Nishikawa et al., 2010) ABSTRACT -Testicular toxicity of chemical substances has been generally assessed by sperm properties and histology. However, the methods can provide only a few information of the mechanism of the toxicity. The aim of this study is to show a method that can evaluate an overview of testicular toxic mechanisms using a tissue-specific microarray and classification of genes using Medical Subject Headings (MeSH). Male ICR mice (6 weeks old) were treated with doxorubicin hydrochloride (0, 0.1, 0.3 mg/kg/ time, three times per week) by subcutaneous injection for 6 weeks (until 11 weeks old). Six weeks after the final administration, tissue and blood samples were obtained. Testes were subjected to gene expression analysis using quantitative RT-PCR and cDNA microarray (testis2). To interpret the microarray data, genes were classified using MeSH related to the functions of testis and sperm. Doxorubicin (both 0.1 and 0.3 mg/kg group) induced a decrease in sperm normal morphology and mortality, daily sperm production, and the number of Sertoli cells in the seminiferous tubules. Quantitative RT-PCR and microarray analysis showed dysregulation of mRNA expression levels of genes related to Sertoli cells, germ cells and spermatogenesis. Analysis of microarray data showed a significant enrichment of a total of ten MeSH categories including Spermatogenesis, Sertoli cells, Germ cells and Male infertility. This article concluded that analysis using testicular specific microarray combined with MeSH showed a more comprehensive overview of testicular toxic mechanisms than existing methods; i.e., examination of sperm properties and the histological examinations.

show abstract

Section: Discussionsupporting

confidence: 93%

Evaluation of testicular toxicology of doxorubicin based on microarray analysis of testicular specific gene expression

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Therefore, the agent interferes with cell proliferation. In the testes of experimental animals, DXR has been shown to disturb spermatogenesis in a dose-dependent manner [12,13]. A number of reports have demonstrated decreases in DXR-induced toxicity using various natural or artificial compounds [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Green tea extracts attenuate doxorubicin-induced spermatogenic disorders in conjunction with higher telomerase activity in mice

Sakai

Sueoka

Tanigaki

et al. 2010

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The aim of this study was to investigate the protective effect of green tea extracts against doxorubicininduced damage in the mouse testes correlating with telomerase activity. Methods Green tea extracts were administered orally. Doxorubicin was coadministered intraperitoneally. These testes were evaluated histologically and the telomerase activity was analyzed. Additional immunostaining was carried out. Results Both the sperm density and sperm motility were significantly increased in green tea extracts coadministration groups as compared to the doxorubicin-treated groups. By histological analysis, germ cell damage was greatly attenuated by green tea extracts coadministration. Telomerase activity significantly increased in association with the coadministration of green tea extracts as compared to that of doxorubicin-only groups. In all groups, human telomerase reverse transcriptase signals were mainly observed in the spermatocytes and spermatids.Conclusions These findings suggest that green tea extracts exert protective effects against doxorubicin-induced spermatogenic disorders in conjunction with higher telomerase activity levels.

show abstract

“…The anthracycline antibiotic doxorubicin, or Adriamycin, is the antineoplasic drug of choice in the treatment of many solid tumors, although one of its adverse effects is male infertility (1)(2)(3). In response to several chemotherapeutic agents including anthracyclines, the number of male germ cells undergoing apoptosis increases several fold (4).…”

Section: Introductionmentioning

confidence: 99%

Doxorubicin Affects Testicular Lipids with Long-Chain (C18-C22) and Very Long-Chain (C24-C32) Polyunsaturated Fatty Acids

2007

View full text Add to dashboard Cite

Doxorubicin disrupts spermatogenesis by causing apoptosis of spermatogonia and primary spermatocytes. The aim of this study was to examine the effect of this agent on adult rat testicular lipids and their fatty acids. A single dose (7.5 mg/kg) and a multidose regime (3 mg/kg once a week for 4 weeks) were evaluated. Both treatments resulted in the gradual loss of spermatogenic cells and determined a marked reduction in testicular size and weight 9 weeks after their start. Germ cell loss was accompanied by a decrease in phospholipids, including glycerophospholipids and sphingomyelin. Concomitantly, glycerophospholipids lost selectively their major polyunsaturated fatty acid (PUFA), 22:5n-6, and sphingomyelin lost its major very long-chain PUFA (VLCPUFA), 28:4n-6 and 30:5n-6. The molecular species from which the lost polyenes originated were thus a trait of germ cells. A transient peak of 16:0-ceramide was observed 48 h after the single dose.

show abstract

Effects of adriamycin, an anticancer drug showing testicular toxicity, on fertility in male rats.

Cited by 40 publications

References 9 publications

Evaluation of testicular toxicology of doxorubicin based on microarray analysis of testicular specific gene expression

Evaluation of testicular toxicology of doxorubicin based on microarray analysis of testicular specific gene expression

Green tea extracts attenuate doxorubicin-induced spermatogenic disorders in conjunction with higher telomerase activity in mice

Doxorubicin Affects Testicular Lipids with Long-Chain (C18-C22) and Very Long-Chain (C24-C32) Polyunsaturated Fatty Acids

Contact Info

Product

Resources

About