Effects of Adventitious Buds Regeneration From Three Kinds of Explants in Jatropha Curcas

Abstract

An efficient method has been established to induce adventitious buds regeneration from 3 kinds of explants (hypocotyl, cotyledon leaf and leaf) of Jatropha curcas L. All the explants were treated by high concentrations (5-120 mg/L) of TDZ solution for different soak period (5-80 min) with the regeneration rate significantly enhanced and the quality of the regenerated buds dramatically improved. The best results of adventitious bud's regeneration were detected when explants were treated with 20 mg/L TDZ solution for 20 min before being inoculated on basic MS medium free of hormone for 30 days. What's more, addition of 0.4 mg/L GA3 into the elongation medium was the most conducive to the extension of adventitious buds. By applying this method described in our paper, regeneration effect of J. curcas was remarkably improved.