Introduction: Increased abdominal fat and sedentary lifestyles contribute to cardiovascular disease risk. Low-intensity electrical current (microcurrent) on the abdominal region, associated with physical exercise, appears to be an innovative method to increase the lipolytic rate of abdominal adipocytes, in order to reduce abdominal fat. This study aimed to analyze the acute effects of microcurrent associated with an aerobic exercise program in healthy subjects in lipolysis. Method: A double-blinded, randomized controlled trial was developed and conducted in a higher education school. Eighty-three healthy subjects, aged between 18 and 30 years old and with a 18.5 to 29.9 kg/m 2 body mass index were randomly assigned either to an experimental or to a placebo group. Subjects received a trans-abdominal microcurrent stimulation for 40 min with (experimental group) or without (placebo group) electrical current, followed by a single aerobic exercise session (60 min at 45-55% VO2max intensity). Lipolytic activity (serum glycerol), abdominal fat (waist circumference, abdominal skinfold, ultrasonography), and serum lipid profile (serum triglyceride, total cholesterol, lowdensity lipoprotein cholesterol and high-density lipoprotein cholesterol) were evaluated in all subjects. Physical activity (International Physical Activity Questionnaire) and dietary intake (food-frequency questionnaire) questionnaires were applied. Results: After the intervention, lipolytic rate was significantly higher (p = 0.003) in the experimental group (mean = 0.15) than in the placebo group (mean = 0.09). Glycerol results showed a statistically significant increase between baseline and after the intervention for both experimental group (p = 0.001) and the placebo group (p = 0.001). Conclusion: Combined use of microcurrent and physical aerobic exercise had an acute effect enhancing lipolytic rate comparing to exercise alone, in young healthy subjects.