Effects of Age and Calcium Ion on Testis Carbonyl Reductase in Rats

Inazu, Norihisa; Fujii, Tomoko

doi:10.1254/jjp.63.65

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…To our knowledge, there are no reports concerning age-related changes of hepatic CBR1 and AKR1C activities and/or expressions. Only Inazu and Fujii [43] reported that activity of rat testicular carbonyl reductase reached a peak value at 3 weeks, then it steeply declined at 4 weeks, and did not further change between 4 and 20 weeks of age. …”

Section: Discussionmentioning

confidence: 99%

Age-Related Changes in Hepatic Activity and Expression of Detoxification Enzymes in Male Rats

Vyskočilová

Szotáková

Skálová

et al. 2013

BioMed Research International

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Process of aging is accompanied by changes in the biotransformation of xenobiotics and impairment of normal cellular functions by free radicals. Therefore, this study was designed to determine age-related differences in the activities and/or expressions of selected drug-metabolizing and antioxidant enzymes in young and old rats. Specific activities of 8 drug-metabolizing enzymes and 4 antioxidant enzymes were assessed in hepatic subcellular fractions of 6-week-old and 21-month-old male Wistar rats. Protein expressions of carbonyl reductase 1 (CBR1) and glutathione S-transferase (GST) were determined using immunoblotting. Remarkable age-related decrease in specific activities of CYP2B, CYP3A, and UDP-glucuronosyl transferase was observed, whereas no changes in activities of CYP1A2, flavine monooxygenase, aldo-keto reductase 1C, and antioxidant enzymes with advancing age were found. On the other hand, specific activity of CBR1 and GST was 2.4 folds and 5.6 folds higher in the senescent rats compared with the young ones, respectively. Interindividual variability in CBR1 activity increased significantly with rising age. We suppose that elevated activities of GST and CBR1 may protect senescent rats against xenobiotic as well as eobiotic electrophiles and reactive carbonyls, but they may alter metabolism of drugs, which are CBR1 and especially GSTs substrates.

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Section: Discussionmentioning

confidence: 99%

Age-Related Changes in Hepatic Activity and Expression of Detoxification Enzymes in Male Rats

Vyskočilová

Szotáková

Skálová

et al. 2013

BioMed Research International

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“…We have found that exogenous calcium has biphasic effects on testicular CR activity in 3-week-old rats but not in older animals (17). The results that Px offspring delays the aging process of hypothalamo-pituitaryovarian axis described by Fujii and Yamamoto (2) are of interest.…”

Section: Discussionmentioning

confidence: 73%

Ovarian Carbonyl Reductase Delayed Onset of Persistent Estrus in the Offspring of Parathyroidectomized Mother Rats

Inazu

Fujii

1995

Japanese Journal of Pharmacology

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ABSTRACT-We investigated the effects of maternal parathyroidectomy on day 5 of pregnancy on the ovarian carbonyl reductase (CR) in the offspring of rats. Changes in the ovarian CR of the offspring were examined at 4 and 8 months of age. In 8-month-old female offspring, the ovarian weight and the ovarian CR activity were significantly higher in rats from parathyroidectomized mothers than in rats from sham-operated mothers, and the offspring of the parathyroidectomized mothers showed the regular 4-day estrous cycles at 8 months of age, while the offspring of the sham-operated mothers were in the state of persistent estrus. Furthermore, intense immunostaining was found in the theca interna cells and the interstitial gland cells in the ovary of rats from the parathyroidectomized mothers, whereas in the ovary of the age-matched normal rats, immunostaining was faint. These results suggest that the maternal parathyroidectomy affect the activity and localization of CR in the ovary of female offspring.Keywords: Carbonyl reductase, Parathyroidectomy in pregnancy, Ovary, Persistent estrus Fujii (1) has reported for the first time that the characteristic response of serum calcium to parathyroid removal in the first (Fl) generation rats from mothers parathyroidectomized on day 5 of pregnancy (Px-F1 rats) is inherited into the third and fourth generations. Moreover, Fujii and Yamamoto (2) have reported that female Px rats show the regular 4-day estrous cycle over 12 months of age, whereas rats from sham-operated mothers (normal rats) show persistent estrus and demonstrated that the aging process of the hypothalamopituitary-ovarian axis in Px rats is delayed. On the other hand, Caplan and Beguin (3) have reported that in patients with hypoparathyroidism, requirements for calcium and vitamin D decline significantly during lactation.We have already purified and characterized carbonyl reductases (CRs, EC 1.1.1.184) from rat ovary (4) and testis (5) and human testis (6). CR catalyzes the NADPH-dependent reduction of a variety of endogenous and xenobiotic carbonyl compounds to the corresponding alcohols, and it is known as prostaglandin 9-keto reductase. Indeed, CRs from rat ovary and testis have both prostaglandin 9-keto reductase and prostaglandin 15-keto reductase activity with comparatively low Km (4, 5). The ovarian CR has been demonstrated to be induced by luteinizing hormone/human chorionic gonadotropin (LH/hCG), and the induction is potentiated by estrogen in immature (7-9) and mature (10, 11) rats. In the present study, we investigated changes in the ovarian CR activity and its content in Px-F1 rats. MATERIALS AND METHODS AnimalsVirgin female Wistar-Imamichi strain rats (8-weeksold) were purchased from the Institute for Animal Reproduction (Ibaragi) and housed in group cages under controlled conditions of light (14 hr on, 10 hr off) and temperature (241C). Food and water were always available. Rats were made pregnant by mating with fertile males, and pregnancy was confirmed by the presence of vaginal sperm on day 0. On day...

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“…Enzyme expression in the ovary is modulated by estrogens and corticosteroids (Inazu et aI., 1990; and Ca 2 +ions and testosterone appear to influence the expression in the testes (Inazu and Fujii, 1993). These findings strongly suggested a role of the rat enzyme in steroid-modulated pathways or steroid metabolism itself.…”

Section: Introductionmentioning

confidence: 99%