Effects of age and concentrate feeding on the expression of MCT 1 and CD147 in the gastrointestinal tract of goats and Hereford finishing beef bulls

Koho, Ninna; Taponen, Juhani; Tiihonen, H.; Manninen, Merja; Pösö, A.R.

doi:10.1016/j.rvsc.2010.06.002

Cited by 10 publications

(22 citation statements)

References 29 publications

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“…As the MCT1 protein exists exclusively in basal cells , Taifor et al 2009), the extreme increase in the number of REC originating from SB in G2 and G3 cell populations from h/c-sheep gives an additional explanation for the observed results. In accordance with our data, bulls fed concentrate ad libitum during the finishing period showed significantly higher abundance of ruminal MCT1 protein than bulls fed only 50 % of their daily ad libitum consumption (Koho et al 2010). Taken into account the functional role of MCT1 for transepithelial SCFA absorption and ruminal fluid as well as REC pH regulation , Kirat et al 2006, Graham et al 2007), our results point to an important role of an increased MCT1 expression for the functional adaptation of rumen epithelium to a high energy diet.…”

Section: Discussionsupporting

confidence: 90%

The mRNA and protein expression of ruminal MCT1 is increased by feeding a mixed hay/concentrate diet compared with hay <i>ad libitum</i> diet (Short Communication)

Kuzinski

Röntgen

2011

Arch. Anim. Breed.

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In this study the protein and mRNA expression of Monocarboxylate transporter 1 (MCT1) was evaluated in rumen epithelial cells (REC) obtained from sheep fed hay ad libitum (control, h diet, n=4) or a mixed hay/concentrate diet (h/c diet, n=4) for two weeks. REC were isolated via fractionated trypsination and three groups consisting of fractions 3 to 5=G1, fractions 6 to 8=G2, and fractions 9 and 10=G3 were formed. Using an anti-basal cytokeratin antibody and flow cytometric analysis, the proportion of REC originating from the stratum basale (SB) was determined for each group. In addition, MCT1 mRNA and protein expression was determined by qRT-PCR and Western blot, respectively. Feeding the h/c diet led to a 299±93 % elevation of the number of SB cells known to express the MCT1 protein. This is accompanied by an increased MCT1 mRNA (1.8 to 2.2-fold) and protein (1.3-fold) expression. Thus, an increased number of MCT1 expressing cells and upregulation of ruminal MCT1 protein seem to be components of rumen epithelium functional adaptation to high energy diet.Keywords: sheep, rumen, epithelial cells, transport protein, high energy diet Zusammenfassung Die mRNA-und Protein-Expression des MCT1 in Pansenepithelzellen wird durch Zufütterung von Konzentrat zu einer ad libitum Heu-Ration erhöhtIn der vorliegenden Studie wurden die mRNA-und Proteinexpression des Monocarboxylattransporters 1 (MCT1) in Pansenepithelzellen (PEZ) von Schafen, die über zwei Wochen entweder Heu ad libitum (h-Diät, n=4) oder eine Heu/Konzentrat-Mischration (h/c-Diät, n=4) erhielten, untersucht. Ovine PEZ wurden mittels fraktionierter Trypsinierung gewonnen und anschließend drei Zellgruppen, bestehend aus den Fraktionen 3-5 (G1), 6-8 (G2) und 9/10 (G3), zugeordnet. Mittels Flowzytometrie und unter Nutzung eines spezifischen Antikörpers gegen basales Cytokeratin wurde der Anteil von PEZ aus dem Stratum basale (SB) für jede Zellgruppe ermittelt. Die Expression von MCT1-mRNA und -Protein wurde mittels qRT-PCR und Western Blot analysiert. Die Anzahl MCT1-exprimierender SB-Zellen, stieg nach Füt-terung der h/c-Diät um 299±93 %. Damit ist eine Erhöhung der MCT1-mRNA-(1,8-2,2-fach) sowie der Proteinexpression (1,3-fach) verbunden. Ein erhöhter Anteil MCT1-expremierender PEZ und die Aufregulation der MCT1 Proteinmenge scheinen somit Komponenten der funktionellen Anpassung des Pansenepithels an energiereiche Fütterung darzustellen.

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Section: Discussionsupporting

confidence: 90%

The mRNA and protein expression of ruminal MCT1 is increased by feeding a mixed hay/concentrate diet compared with hay <i>ad libitum</i> diet (Short Communication)

Kuzinski

Röntgen

2011

Arch. Anim. Breed.

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“…This idea is supported by previous studies in ruminants (Koho et al, 2011;Kuzinski and Rontgen, 2011;Metzler-Zebeli et al, 2013) where an increase of MCT1 expression was detected following dietary treatments that enhanced the amount of fermentable substrates and consequently the amount of VFA produced. Considering that at 28 DIM we detected a higher expression of both MCT1 and HMGCS2, it is possible that an increase in concentration of ketone bodies within the ruminal epithelial cell led to an increase in export into the bloodstream.…”

Section: Gene Expression In Ruminal Epithelium During Transitionsupporting

confidence: 80%

Abundance of ruminal bacteria, epithelial gene expression, and systemic biomarkers of metabolism and inflammation are altered during the peripartal period in dairy cows

Minuti

Palladino

Khan

et al. 2015

Journal of Dairy Science

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Seven multiparous Holstein cows with a ruminal fistula were used to investigate the changes in rumen microbiota, gene expression of the ruminal epithelium, and blood biomarkers of metabolism and inflammation during the transition period. Samples of ruminal digesta, biopsies of ruminal epithelium, and blood were obtained during -14 through 28d in milk (DIM). A total of 35 genes associated with metabolism, transport, inflammation, and signaling were evaluated by quantitative reverse transcription-PCR. Among metabolic-related genes, expression of HMGCS2 increased gradually from -14 to a peak at 28 DIM, underscoring its central role in epithelial ketogenesis. The decrease of glucose and the increase of nonesterified fatty acids and β-hydroxybutyrate in the blood after calving confirmed the state of negative energy balance. Similarly, increases in bilirubin and decreases in albumin concentrations after calving were indicative of alterations in liver function and inflammation. Despite those systemic signs, lower postpartal expression of TLR2, TLR4, CD45, and NFKB1 indicated the absence of inflammation within the epithelium. Alternatively, these could reflect an adaptation to react against inducers of the immune system arising in the rumen (e.g., bacterial endotoxins). The downregulation of RXRA, INSR, and RPS6KB1 between -14 and 10 DIM indicated a possible increase in insulin resistance. However, the upregulation of IRS1 during the same time frame could serve to restore sensitivity to insulin of the epithelium as a way to preserve its proliferative capacity. The upregulation of TGFB1 from -14 and 10 DIM coupled with upregulation of both EGFR and EREG from 10 to 28 DIM indicated the existence of 2 waves of epithelial proliferation. However, the downregulation of TGFBR1 from -14 through 28 DIM indicated some degree of cell proliferation arrest. The downregulation of OCLN and TJP1 from -14 to 10 DIM indicated a loss of tight-junction integrity. The gradual upregulation of membrane transporters MCT1 and UTB to peak levels at 28 DIM reflected the higher intake and fermentability of the lactation diet. In addition, those changes in the diet after calving resulted in an increase of butyrate and a decrease of ruminal pH and acetate, which partly explain the increase of Anaerovibrio lipolytica, Prevotella bryantii, and Megasphaera elsdenii and the decrease of fibrolytic bacteria (Fibrobacter succinogenes, Butyrivibrio proteoclasticus). Overall, these multitier changes revealed important features associated with the transition into lactation. Alterations in ruminal epithelium gene expression could be driven by nutrient intake-induced changes in microbes; microbial metabolism; and the systemic metabolic, hormonal, and immune changes. Understanding causes and mechanisms driving the interaction among ruminal bacteria and host immunometabolic responses merits further study.

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“…The very early onset of MCT1 expression found in the studies of Koho et al. (, ) thus indicates that MCT1 expression might be induced independently of SCFA.…”

Section: Introductionmentioning

confidence: 85%

“…Koho et al. (, ) determined the expression of MCT1 in the rumen of developing reindeer and goats and demonstrated that MCT1 expression in the rumen epithelium raised post‐natally. The authors assumed a beginning SCFA release in the developing rumen to be the main inductor of MCT1 expression.…”

Section: Introductionmentioning

confidence: 99%

Post‐natal changes inMCT1 expression in the forestomach of calves

Pfannkuche

Taifour

Steinhoff‐Wagner

et al. 2013

Animal Physiology Nutrition

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The monocarboxylate transporter 1 (MCT1) has been demonstrated to be involved in the transfer of short-chain fatty acids (SCFA) and/or their intraepithelial metabolites from the rumen to the blood. As MCT1 plays a role in SCFA transfer, it is assumed that SCFA are the main substrates influencing its expression. However, there are hints that MCT1 may also be expressed during the early life of the animal when SCFA are not released in the forestomach. To figure out whether MCT1 expression in the forestomach is influenced independently of SCFA during that period, we studied post-natal MCT1 expression immunohistochemically in the epithelia of omasum, atrium ruminis, saccus dorsalis ruminis, saccus ventralis ruminis and reticulum of calves born preterm and at term. The calves were nourished by colostrum or by milk-based formula diet. MCT1 could be found in all the forestomach compartments tested, even in preterm calves. The protein was mainly oriented to the luminal side in the immature epithelium 24 h after birth. Orientation to the blood side of the cells developed during the first 4 days after birth. In the rumen epithelia (but not in the other forestomach compartments tested), orientation of MCT1 to the blood side of the cells was paralleled by an increase in the overall expression rate during the first 4 days after birth. As lactate levels were very high directly after birth, a lactate-dependent substrate induction may have been the underlying mechanism. However, non-specific changes due to general differential processes might also be the cause. Both early upregulation of MCT1 and high blood lactate levels may provide the epithelia with lactate as energy source.

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Effects of age and concentrate feeding on the expression of MCT 1 and CD147 in the gastrointestinal tract of goats and Hereford finishing beef bulls

Cited by 10 publications

References 29 publications

The mRNA and protein expression of ruminal MCT1 is increased by feeding a mixed hay/concentrate diet compared with hay <i>ad libitum</i> diet (Short Communication)

The mRNA and protein expression of ruminal MCT1 is increased by feeding a mixed hay/concentrate diet compared with hay <i>ad libitum</i> diet (Short Communication)

Abundance of ruminal bacteria, epithelial gene expression, and systemic biomarkers of metabolism and inflammation are altered during the peripartal period in dairy cows

Post‐natal changes inMCT1 expression in the forestomach of calves

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Effects of age and concentrate feeding on the expression of MCT 1 and CD147 in the gastrointestinal tract of goats and Hereford finishing beef bulls

Cited by 10 publications

References 29 publications

The mRNA and protein expression of ruminal MCT1 is increased by feeding a mixed hay/concentrate diet compared with hay &lt;i&gt;ad libitum&lt;/i&gt; diet (Short Communication)

The mRNA and protein expression of ruminal MCT1 is increased by feeding a mixed hay/concentrate diet compared with hay &lt;i&gt;ad libitum&lt;/i&gt; diet (Short Communication)

Abundance of ruminal bacteria, epithelial gene expression, and systemic biomarkers of metabolism and inflammation are altered during the peripartal period in dairy cows

Post‐natal changes inMCT1 expression in the forestomach of calves

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The mRNA and protein expression of ruminal MCT1 is increased by feeding a mixed hay/concentrate diet compared with hay <i>ad libitum</i> diet (Short Communication)

The mRNA and protein expression of ruminal MCT1 is increased by feeding a mixed hay/concentrate diet compared with hay <i>ad libitum</i> diet (Short Communication)