Effects of age and sex on the expression of estrogen receptor α and β in the mouse inner ear

Motohashi, Ray; Takumida, Masaya; Shimizu, Akira; Konomi, Ujimoto; Fujita, Koji; Hirakawa, Katsuhiro; Suzuki, Mamoru; Anniko, Matti

doi:10.1080/00016480903016570

Cited by 12 publications

(19 citation statements)

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“…That said, marginal cell loss did not seem to explain prominent gender differences in CBA/CaJ, and gender effects on marginal cell function must be considered. The idea that estrogen impacts strial marginal cell operation is supported by the literature (Konig et al 2008;Motohashi et al 2010). Interestingly, the effect appears to be one of inhibition (Lee and Marcus 2001).…”

Section: The Role Of Gender In Hearing and Ep Declinesupporting

confidence: 70%

Divergent Aging Characteristics in CBA/J and CBA/CaJ Mouse Cochleae

Ohlemiller

Dahl

Gagnon

2010

JARO

100

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Two inbred mouse strains, CBA/J and CBA/CaJ, have been used nearly interchangeably as 'good hearing' standards for research in hearing and deafness. We recently reported, however, that these two strains diverge after 1 year of age, such that CBA/CaJ mice show more rapid elevation of compound action potential (CAP) thresholds at high frequencies (Ohlemiller, Brain Res. 1277: 70-83, 2009). One contributor is progressive decline in endocochlear potential (EP) that appears only in CBA/CaJ. Here, we explore the cellular bases of threshold and EP disparities in old CBA/J and CBA/CaJ mice. Among the major findings, both strains exhibit a characteristic age (∼18 months in CBA/J and 24 months in CBA/CaJ) when females overtake males in sensitivity decline. Strain differences in progression of hearing loss are not due to greater hair cell loss in CBA/ CaJ, but instead appear to reflect greater neuronal loss, plus more pronounced changes in the lateral wall, leading to EP decline. While both male and female CBA/CaJ show these pathologies, they are more pronounced in females. A novel feature that differed sharply by strain was moderate loss of outer sulcus cells (or 'root' cells) in spiral ligament of the upper basal turn in old CBA/CaJ mice, giving rise to deep indentations and void spaces in the ligament. We conclude that CBA/CaJ mice differ both quantitatively and qualitatively from CBA/J in age-related cochlear pathology, and model different types of presbycusis.

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Section: The Role Of Gender In Hearing and Ep Declinesupporting

confidence: 70%

Divergent Aging Characteristics in CBA/J and CBA/CaJ Mouse Cochleae

Ohlemiller

Dahl

Gagnon

2010

JARO

100

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“…Moreover, deafness has been associated with variants in the human gene in both a boy and a girl. A brain‐generated oestrogen binding to intracellular estrogen receptors at PSDs has been described because of its neurotrophic and neuroprotective actions in both males and females (Lee & McEwen, ; Motohashi et al , ; Pinaud & Tremere, ). Our finding of no significant abnormalities other than deafness in either mice or the children with Wbp2 deficiency suggests that Wbp2 might have a specific effect on the transcription of the intracellular estrogen receptors in the auditory system rather than on the hormonal receptors regulating gonadal functions.…”

Section: Discussionmentioning

confidence: 99%

“…In the auditory system, oestrogen is known to control central and peripheral auditory processing (Hultcrantz et al, 2006). Esr1 and Esr2 are expressed in both IHCs and OHCs (Motohashi et al, 2010), and they protect against neuropathy following acoustic trauma (Meltser et al, 2008;Simonoska et al, 2009).…”

Section: Steroid Hormones and Synaptopathymentioning

confidence: 99%

Wbp2 is required for normal glutamatergic synapses in the cochlea and is crucial for hearing

et al. 2016

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WBP2 encodes the WW domain-binding protein 2 that acts as a transcriptional coactivator for estrogen receptor a (ESR1) and progesterone receptor (PGR). We reported that the loss of Wbp2 expression leads to progressive high-frequency hearing loss in mouse, as well as in two deaf children, each carrying two different variants in the WBP2 gene. The earliest abnormality we detect in Wbp2-deficient mice is a primary defect at inner hair cell afferent synapses. This study defines a new gene involved in the molecular pathway linking hearing impairment to hormonal signalling and provides new therapeutic targets.

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“…Our current understanding of the prevalence of multiple ER, AR, and CR isoforms within the ear of a single vertebrate species, however, is incomplete. Indeed, only recently have investigators begun to study changes in receptor expression as a potential mechanism for auditory plasticity [8,25]. To fully understand how steroid hormones might influence vertebrate inner ear function, we measured the expression patterns of all known ERs, ARs, and CRs in a single species, along with circulating gonadal and stress hormone levels.…”

Section: Introductionmentioning

confidence: 99%

Steroid receptor expression in the fish inner earvaries with sex, social status, and reproductive state

Maruska

Fernald

2010

BMC Neurosci

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BackgroundGonadal and stress-related steroid hormones are known to influence auditory function across vertebrates but the cellular and molecular mechanisms responsible for steroid-mediated auditory plasticity at the level of the inner ear remain unknown. The presence of steroid receptors in the ear suggests a direct pathway for hormones to act on the peripheral auditory system, but little is known about which receptors are expressed in the ear or whether their expression levels change with internal physiological state or external social cues. We used qRT-PCR to measure mRNA expression levels of multiple steroid receptor subtypes (estrogen receptors: ERα, ERβa, ERβb; androgen receptors: ARα, ARβ; corticosteroid receptors: GR2, GR1a/b, MR) and aromatase in the main hearing organ of the inner ear (saccule) in the highly social African cichlid fish Astatotilapia burtoni, and tested whether these receptor levels were correlated with circulating steroid concentrations.ResultsWe show that multiple steroid receptor subtypes are expressed within the main hearing organ of a single vertebrate species, and that expression levels differ between the sexes. We also show that steroid receptor subtype-specific changes in mRNA expression are associated with reproductive phase in females and social status in males. Sex-steroid receptor mRNA levels were negatively correlated with circulating estradiol and androgens in both males and females, suggesting possible ligand down-regulation of receptors in the inner ear. In contrast, saccular changes in corticosteroid receptor mRNA levels were not related to serum cortisol levels. Circulating steroid levels and receptor subtype mRNA levels were not as tightly correlated in males as compared to females, suggesting different regulatory mechanisms between sexes.ConclusionsThis is the most comprehensive study of sex-, social-, and reproductive-related steroid receptor mRNA expression in the peripheral auditory system of any single vertebrate. Our data suggest that changes in steroid receptor mRNA expression in the inner ear could be a regulatory mechanism for physiological state-dependent auditory plasticity across vertebrates.

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Effects of age and sex on the expression of estrogen receptor α and β in the mouse inner ear

Cited by 12 publications

References 0 publications

Divergent Aging Characteristics in CBA/J and CBA/CaJ Mouse Cochleae

Divergent Aging Characteristics in CBA/J and CBA/CaJ Mouse Cochleae

Wbp2 is required for normal glutamatergic synapses in the cochlea and is crucial for hearing

Steroid receptor expression in the fish inner earvaries with sex, social status, and reproductive state

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