Effects of Age on c-fos and c-myc Gene Expression in Response to Hemodynamic Stress in Isolated, Perfused Rat Hearts

Shida, Mikio; Isoyama, Shogen

doi:10.1006/jmcc.1993.1115

Cited by 32 publications

(10 citation statements)

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“…Map3k6 (a signal‐regulated kinase of the JNK pathway) and junb (a downstream target of stress‐activated signaling) both exhibit higher levels of normalized expression after paraquat injection in the young mice when compared with the old. Previous studies agree with our findings, having shown reduced levels of expression of the IEGs c‐fos , c‐myc , and c‐jun in aged rat hearts after hemodynamic stress 27,28. It is conceivable that the observed early increase in expression of JNK‐related genes in the young animals provides increased protection in the heart against induced oxidative stress as compared with that of their old counterparts.…”

Section: Discussionsupporting

confidence: 92%

Impairment of the Transcriptional Responses to Oxidative Stress in the Heart of Aged C57BL/6 Mice

Edwards

Sarkar

Klopp

et al. 2004

Annals of the New York Academy of Sciences

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To investigate the transcriptional response to oxidative stress in the heart and how it changes with age, we examined the cardiac gene expression profiles of young (5 months old), middle-aged (15 months old), and old (25 months old) C57BL/6 mice treated with a single intraperitoneal injection of paraquat (50 mg/kg). Mice were killed at 0, 1, 3, 5, and 7 hours after paraquat treatment, and the gene expression profile was obtained with high-density oligonucleotide microarrays. Of 9,977 genes represented on the microarray, 249 transcripts in the young mice, 298 transcripts in the middle-aged mice, and 256 transcripts in the old mice displayed a significant change in mRNA levels (ANOVA, P <.01). Among these, a total of 55 transcripts were determined to be paraquat responsive for all age groups. Genes commonly induced in all age groups include those associated with stress, inflammatory, immune, and growth factor responses. Interestingly, only young mice displayed a significant increase in expression of all three isoforms of GADD45, a DNA damage-responsive gene. Additionally, the number of immediate early genes found to be induced by paraquat was considerably higher in the younger animals. These results demonstrate that, at the transcriptional level, there is an age-related impairment of specific inducible pathways in the response to oxidative stress in the mouse heart.

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Section: Discussionsupporting

confidence: 92%

Impairment of the Transcriptional Responses to Oxidative Stress in the Heart of Aged C57BL/6 Mice

Edwards

Sarkar

Klopp

et al. 2004

Annals of the New York Academy of Sciences

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“…At the gene expression level, heart aging has been shown to be associated with an augmentation of cardiac IL-6 induction following endotoxin challenge [34] and reduced expression of immediate early genes (IEGs) c-fos, c-myc, and c-jun following hemodynamic stress [35] and pressure overload [36]. In order to learn more about the specific pathways that are induced by oxidative stress, and how this response is affected by aging, we treated young (5 months old), middleaged (15 months old), and aged (30 months old) mice with the ROS generator Paraquat (50 mg/kg of body weight) and sacrificed animals at 1, 3, 5, and 7 h after injection [37].…”

Section: Age-related Impairment Of the Transcriptional Responses To Omentioning

confidence: 99%

Gene expression profiling studies of aging in cardiac and skeletal muscles

Prolla¹

2005

Cardiovascular Research

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To examine transcriptional alterations associated with aging in skeletal muscle and the heart, we and others have used DNA microarrays to compare the gene expression profile of young and old animals. Aging results in a differential gene expression pattern specific to each tissue, and most alterations can be completely or partially prevented by caloric restriction (CR) in both heart and skeletal muscle. Transcriptional patterns of tissues from calorie-restricted animals suggests that CR retards the aging process by reducing endogenous damage and by inducing metabolic shifts associated with specific transcriptional profiles. These studies demonstrate that DNA microarrays can be used in cardiovascular aging research to generate panels of hundreds of transcriptional biomarkers, providing a new tool to measure biological age of cardiac and skeletal muscles and to test interventions designed to retard aging in these tissues.

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“…The acute induction of both immediate-early genes and later responding genes that are expressed during the hypertrophic response is blunted in hearts of aged rats after aortic constriction. 79,80 Similarly, the acute induction of heat shock 70 protein genes in response to either ischemia or heat shock is reduced in hearts of senescent rats. 81,82 A similar loss of adaptive capacity is observed in younger rats that have used a part of their reserve capacity before a growth factor challenge.…”

Section: Reduced Chronic Adaptive Capacity Of the Older Heartmentioning

confidence: 99%

Arterial and Cardiac Aging: Major Shareholders in Cardiovascular Disease Enterprises

2003

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Effects of Age on c-fos and c-myc Gene Expression in Response to Hemodynamic Stress in Isolated, Perfused Rat Hearts

Cited by 32 publications

References 0 publications

Impairment of the Transcriptional Responses to Oxidative Stress in the Heart of Aged C57BL/6 Mice

Impairment of the Transcriptional Responses to Oxidative Stress in the Heart of Aged C57BL/6 Mice

Gene expression profiling studies of aging in cardiac and skeletal muscles

Arterial and Cardiac Aging: Major Shareholders in Cardiovascular Disease Enterprises

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