Effects of ageing on nuclear DNA integrity and metabolism in mussel cells ( Mytilus edulis L.)

Accomando, R.; Viarengo, Aldo; Moore, Michael N.; Malinowska, Barbara

doi:10.1016/s1357-2725(98)00154-x

Cited by 11 publications

(5 citation statements)

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“…In addition, lysosomes can interfere with DNA integrity through their role in ROS production, as the intra‐lysosomal environment is a site of oxyradical production, and the destabilization of lysosomal membranes may result in the release of oxyradicals [Livingstone,2001]. Thus, DNA strand breakages as a consequence of oxidative stress due to metal bioaccumulation may be related to the induction of MN and NB in mussels from these metal polluted sites, as single and double‐strand breakages have been signalled as one of the reasons for MN generation occurring [Black et al,1996; Accomando et al,1999; Akcha et al,2000b; Almeida et al,2005; Stambuk et al,2008; Shi et al,2009]. In addition, positive correlations detected beetwen the frequencies of MN, NB and the levels of some antioxidant enzymes (SOD, SeGP, GR) (Table II) supported the involvement of an increased production of ROS in the DNA damage detected.…”

Section: Discussionmentioning

confidence: 99%

“…Besides, some compounds may inhibit the mitotic activity, resulting in a low capacity of MN induction [Majone et al,1987; Wrisberg et al,1992; Dolcetti and Venier,2002; Venier et al,2003]. Furthermore, as a cause‐effect relationship between DNA strand breakages (reversible genetic damage) and the generation of MN (irreversible damage) has been reported in mussels [Accomando et al,1999; Akcha et al,2000b; Almeida et al,2005; Stambuk et al,2008], faster and more efficient DNA repair systems found in chronically exposed organisms [Siu et al,2004a; Black et al,1996] may prevent strand breakages from being converted into MN. In addition, the influence of low water temperatures in the generation of MN and NB in mussels from these sites, especially in mussels from Barcelona, Vallcarca, and Tarragona (16.6, 18.5, and 17.2°C, respectively), cannot be ruled out.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Micronuclei and other nuclear abnormalities in mussels (Mytilus galloprovincialis) as biomarkers of cyto‐genotoxic pollution in mediterranean waters

Fernández

Campillo

Martínez-Gómez

et al. 2011

Environ and Mol Mutagen

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Environmental genotoxicity and cytotoxicity along the Spanish Mediterranean coast was investigated through the determination of levels of micronuclei (MN) and other nuclear abnormalities (NAs) such as nuclear buds (NB) and binucleated cells (BN) in gills of wild mussels, Mytilus galloprovincialis, from 17 study sites. The results obtained were studied in relation to the exposure to main pollutants (metals, PAHs, PCBs and DDTs), gill antioxidant enzyme activities and environmental variables (temperature and salinity). The highest MN and NB levels were found in mussels from metal-polluted sites, such as Cartagena (MN: 11.6‰, NB: 4.6‰) and Portman (MN: 8.0‰, NB: 3.5‰), where genotoxicity seemed to be related to the oxidative stress generated by exposure to metals. High frequencies of MN and NB in mussels from Algeciras 1 (MN: 8.6‰, NB: 4‰) and of MN (7.2‰) in mussels from Manilva also indicated genotoxicity, though not attributable to the pollutants analysed. In contrast, MN levels at sites highly polluted by organic contaminants such as Barcelona, Vallcarca, Tarragona and Valencia, were lower than expected (ranging from 3.8 to 5.8‰). On the other hand, evidences of cytotoxicity indicated by the high frequencies of BN found in mussels from Vallcarca, Barcelona, and Cartagena (3.9, 3.4, and 2.3‰, respectively) appeared to be specifically related to the accumulation of organic pollutants. Positive correlations found between MN and NB frequencies, and also between both NAs and water temperature, suggested that they were related genotoxic events, and that this environmental factor is able to influence their levels and has to be taken into account in the application of these biomarkers in field studies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Micronuclei and other nuclear abnormalities in mussels (Mytilus galloprovincialis) as biomarkers of cyto‐genotoxic pollution in mediterranean waters

Fernández

Campillo

Martínez-Gómez

et al. 2011

Environ and Mol Mutagen

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“…The existing data indicate DNA damage intensity to differ between species, and DNA repair capacity in bivalves to correlate inversely with age [52,53] . Accomando et al [52] investigated DNA repair activity and damage in different age classes of Mytilus edulis and found higher DNA damage and lower DNA polymerase activity in the older age classes. In line with this, Pruski and Dixon [54] found a higher DNA repair capacity in young hydrothermal vent bivalves (Bathymodiolus azoricus) compared to older individuals.…”

Section: Dna Repairmentioning

confidence: 99%

Masters of Longevity: Lessons from Long-Lived Bivalves – A Mini-Review

2009

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The individual ages of bivalve molluscs can be inferred from the age rings laid down every year in the shell, especially in species inhabiting areas with seasonal variability in environmental factors such as food supply and temperature. Animals obtained from different environmental settings can therefore be used to investigate how specific environmental factors shape the process of ageing in this animal class. Some bivalves have extraordinary long life spans. Species like the ocean quahog Arctica islandica and the freshwater pearl mussel Margaritifera margaritifera live for over hundreds of years. Few studies so far have attempted to study the process of ageing, either specifically in long-lived bivalves or generally in very long-lived species. This review summarizes the current knowledge of cellular ageing in bivalves with a focus on the antioxidant system, as well as tissue repair and metabolic capacities of extremely long-lived species. We discuss the applicability of these animals as models for different ageing theories. We recommend a focus of future research on the molecular mechanisms potentially involved in supporting longevity in these species, including evolutionary old cellular mechanisms such as autophagy and apoptosis, as well as diverse cellular repair pathways.

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“…Occupational exposure to xenobiotics might have resulted in their covalent binding to DNA, which might lead to chromosome alterations and could be an initial event in the process of chemical carcinogenesis (Fairbairn et al, 1995;Shah et al, 1997). Comparably fewer studies have been conducted with aquatic invertebrates and these have been restricted to bivalve species such as the marine mussel (Mytilus edulis) (Accomando et al, 1999), the zebra mussel (Dreissena polymorpha) (Pavlica et al, 2001), Mediterranean mussel (Mytilus galloprovincialis) (Frenzilli et al, 2001) and oyster (Crassostrea americanus) (Nacci et al, 1996). This assay has been carried out in fishes such as bullhead (Ameriurus nebulosus) Common carp (Cyprinus carpio) (Pandrangi et al, 1995), brown trout (Salmo trutta) (Belpaeme et al, 1996), flounder (Pleuronectes americanus) (Nacci et al, 1996), rainbow trout (Oncorhynchus mykiss) (Devaux et al, 1999), butterfish (Pholis gunnellus) (Bombail et al, 2001), zebra fish (Danio rerio) (Schnurstein and Braunbeak, 2001) tilapia (Tilapia mossambica) (Banu et al 2001) and Channa punctatus (Pandey et al, 2006) etc.…”

Section: Comet Assaysmentioning

confidence: 99%