Effects of aging on .ALPHA.1-adrenoceptor mechanisms in the isolated mouse aortic preparation

Tanaka, Yoshio; Funabiki, Midori; Michikawa, Hiromi; Koike, Kenichi

doi:10.1540/jsmr.42.131

Cited by 6 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This reduced contractility was completely reversed in the presence of L-NNA to inhibit NOS suggesting that it was attributable to an age-related increase in the bioavailability of NO. Similar age-related changes in arterial contractility and NO sensitivity have previously been demonstrated [33-36] although not ubiquitously [19, 37-42]. Mouse mesenteric artery eNOS expression has been reported to be unchanged with ageing suggesting that alterations in eNOS activity may underlie the changes in NO-sensitivity [43].…”

Section: Discussionsupporting

confidence: 53%

“…Ageing in caveolin-1 KO mice was associated with an increase in passive diameter (Ci) and a reduction in cross sectional area (Cii). sensitivity have previously been demonstrated [33][34][35][36] although not ubiquitously [19,[37][38][39][40][41][42]. Mouse mesenteric artery eNOS expression has been reported to be unchanged with ageing suggesting that alterations in eNOS activity may underlie the changes in NO-sensitivity [43].…”

Section: Contractile Changes With Ageing Of Wt Micementioning

confidence: 99%

See 1 more Smart Citation

Age‐related changes in the contractile and passive arterial properties of murine mesenteric small arteries are altered by caveolin‐1 knockout

Hausman

Martin

Taggart

et al. 2012

J Cellular Molecular Medi

View full text Add to dashboard Cite

Caveolin-1, an integral protein of caveolae, is associated with multiple cardiovascular signalling pathways. Caveolin-1 knockout (KO) mice have a reduced lifespan. As changes in artery structure and function are associated with ageing we have investigated the role of caveolin-1 ablation on age-related changes of small artery contractility and passive mechanical properties. Mesenteric small arteries isolated from 3 and 12-month wild-type (WT) and caveolin-1 KO mice were mounted on a pressure myograph and changes in passive and functional arterial properties were continuously monitored. In WT mice ageing was associated with a reduction in arterial contractility to noradrenaline which was reversed by inhibition of nitric oxide synthase with L-NNA. Similarly, in 3-month-old mice, caveolin-1 KO reduced contractility to noradrenaline by an L-NNA-sensitive mechanism. However, ageing in caveolin-1 KO mice was not associated with any further change in contractility. In WT mice ageing was associated with an increased passive arterial diameter and cross-sectional area (CSA), consistent with outward remodelling of the arterial wall, and a reduced arterial distensibility. Caveolin-1 ablation at 3 months of age resulted in similar changes in passive arterial properties to those observed with ageing in WT animals. However, ageing in caveolin-1 KO mice resulted in a reduced arterial CSA indicating different effects on passive structural characteristics from that observed in WT mice. Thus, caveolin-1 mice show abnormalities of small mesenteric artery function and passive mechanical characteristics indicative of premature vascular ageing. Moreover, caveolin-1 ablation modulates the age-related changes usually observed in mesenteric arteries of WT mice.

show abstract

Section: Discussionsupporting

confidence: 53%

Section: Contractile Changes With Ageing Of Wt Micementioning

confidence: 99%

Age‐related changes in the contractile and passive arterial properties of murine mesenteric small arteries are altered by caveolin‐1 knockout

Hausman

Martin

Taggart

et al. 2012

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…Aging influences the action and/or efficacy of many signaling molecules, including norepinephrine and the more well-studied gasotransmitter NO (Lakatta 1993;Tanaka et al 2006;van der Loo et al 2000). A recent study of plasma H 2 S concentration in humans over 50-80 years of age reported that H 2 S levels may decline with age ), but whether aging affects the H 2 S signaling system is unknown.…”

Section: Introductionmentioning

confidence: 99%

The hydrogen sulfide signaling system: changes during aging and the benefits of caloric restriction

Predmore

Alendy

Ahmed

et al. 2010

AGE

View full text Add to dashboard Cite

Hydrogen sulfide gas (H 2 S) is a putative signaling molecule that causes diverse effects in mammalian tissues including relaxation of blood vessels and regulation of perfusion in the liver, but the effects of aging on H 2 S signaling are unknown. Aging has negative impacts on the cardiovascular system. However, the liver is more resilient with age. Caloric restriction (CR) attenuates affects of age in many tissues. We hypothesized that the H 2 S signaling system is negatively affected by age in the vasculature but not in the liver, which is typically more resilient to age, and that a CR diet minimizes the age affect in the vasculature. To investigate this, we determined protein and mRNA expression of the H 2 S-producing enzymes cystathionine γ-lyase (CSE) and cystathionine β-synthase (CBS), H 2 S production rates in the aorta and liver, and the contractile response of aortic rings to exogenous H 2 S. Tissue was collected from Fisher 344×Brown Norway rats from 8-38 months of age, which had been maintained on an ad libitum (AL) or CR diet. The results demonstrate that age and diet have differential effects on the H 2 S signaling system in aorta and liver. The aorta showed a sizeable effect of both age and diet, whereas the liver only showed a sizeable effect of diet. Aortic rings showed increased contractile sensitivity to H 2 S and increased protein expression of CSE and CBS with age, consistent with a decrease in H 2 S concentration with age. CR appears to benefit CSE and CBS protein in both aorta and liver, potentially by reducing oxidative stress and ameliorating the negative effect of age on H 2 S concentration. Therefore, CR may help maintain the H 2 S signaling system during aging.

show abstract

“…In this line, the potency of noradrenaline increased in the isolated aorta from rat and mouse with age from 3 to 10 weeks, but decreased thereafter from 10 to 40 weeks (Satoh et al, 1995;Tanaka et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Aging and total stenosis triggers differential responses of carotid and basilar arteries to endothelin-1 and phenylephrine

Andrade

Corrêa

Oliveira

2009

J. Smooth Muscle Res.

View full text Add to dashboard Cite

Our aim was to investigate the effects of ageing on the vascular contractility of carotid and basilar arteries from guinea-pigs, in a model of total stenosis. Moreover, we attempted to identify whether total stenosis of the left common carotid (stenosed) in adult guinea-pigs, would affect the contractions of contralateral carotid (intact) and basilar arteries to different vasoconstrictors. With this purpose, the left carotid was occluded with a silk thread at a position close to its origin. Vascular reactivity experiments using standard muscle bath were performed 7, 15, 30, and 90 days after carotid occlusion. Reactivity of carotid and basilar arteries to endothelin-1, phenylephrine and KCl was reduced with ageing in naive guinea-pigs. The endothelin-1 and KCl-induced contractions were unaltered in arteries from SHAM-operated animals. Moreover, phenylephrine-induced contractions were reduced in both carotids from 7 days SHAM-operated guinea-pigs, when compared to naive group. Stenosis induced progressive reduction in the contraction induced by endothelin-1, phenylephrine and KCl in the stenosed carotid, when compared to their respective age-matched naive and SHAM groups. Interestingly, an increased contractile-response to vasoconstrictor agents in all the contralateral carotids was observed. Stenosis (30 and 90 days) also induced an increase in the contractions induced by endothelin-1 in the basilar artery. Conversely, phenylephrine and KCl-induced contractions were reduced in basilar arteries 7, 15, 30 and 90 days after stenosis. These results showed that stenosis in adult guinea-pigs induce alterations of vascular reactivity in arteries distant from the site of injury. Thus, in spite of the common use of contralateral carotid as control, it must be aware of the potential alteration induced by stenosis in the vascular motility of such vessels. Additionally, it was verified a relationship between the period of stenosis and the alterations in the vascular reactivity to these vasoconstrictors.

show abstract

Effects of aging on .ALPHA.1-adrenoceptor mechanisms in the isolated mouse aortic preparation

Cited by 6 publications

References 19 publications

Age‐related changes in the contractile and passive arterial properties of murine mesenteric small arteries are altered by caveolin‐1 knockout

Age‐related changes in the contractile and passive arterial properties of murine mesenteric small arteries are altered by caveolin‐1 knockout

The hydrogen sulfide signaling system: changes during aging and the benefits of caloric restriction

Aging and total stenosis triggers differential responses of carotid and basilar arteries to endothelin-1 and phenylephrine

Contact Info

Product

Resources

About