With the recent increase in food demand, urban agriculture has gained attention as a way of increasing food self-sufficiency and providing recreational spaces in cities. In this study, the suitability of rooftop greenhouses (RGs), a type of urban agriculture, was analyzed by combining the analytical hierarchy process (AHP) and geographic information systems (GIS) in Seoul, the capital city of Korea. To achieve this, we derived location suitability factors through expert consultations and calculated the weights of each factor through AHP. After building the spatial data according to these factors, they were weighted and summed then scaled to a score of 0–100. The highest weight of the RG location factors was for benefit (0.1782), followed by officially assessed land prices (0.0913) and supermarket density (0.0802). The weights of supermarket density and accessibility were high because they are considered the main distribution channels. When analyzing the location of RGs by linking these results with the spatial data according to factor, we revealed that Gangseo-gu (a district of Seoul) had relatively high location suitability scores. This trend was determined to be caused by the rather low officially assessed land price, high supermarket density, and productive population. This result could prove useful when selecting the approximate locations of RGs in Seoul and for promoting food self-sufficiency in cities.