Effects of ALA-mediated photodynamic therapy on the invasiveness of human glioma cells

Hirschberg, Henry; Sun, Carlos; Krasieva, Tatiana B.; Madsen, Steen J.

doi:10.1002/lsm.20445

Cited by 29 publications

(22 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MCTS cultures have been subjected to microfluidic perfusion to evaluate the impact of interstitial pressure and shear force on tumor cells [109][110][111]. Spheroids have also been embedded within ECM hydrogels, such as BME/Matrigel or collagen I, as a physiological model for metastasis [112][113][114][115][116][117]. The BME/Matrigel hydrogel provides a physical scaffold for the cells to adhere to and move along.…”

Section: Applications and Uses Of Bme/matrigel In Spheroid Culturementioning

confidence: 99%

Matrigel: From discovery and ECM mimicry to assays and models for cancer research

Benton

Arnaoutova

George

et al. 2014

Advanced Drug Delivery Reviews

377

303

View full text Add to dashboard Cite

Section: Applications and Uses Of Bme/matrigel In Spheroid Culturementioning

confidence: 99%

Matrigel: From discovery and ECM mimicry to assays and models for cancer research

Benton

Arnaoutova

George

et al. 2014

Advanced Drug Delivery Reviews

377

303

View full text Add to dashboard Cite

“…2f). The ability of radial growth of spheroids has been previously reported as a parameter to describe the migratory behavior of the spheroid cells and can be associated with a more metastatic phenotype [45,46]. In the present report, it was demonstrated that photodynamic intervention suppressed the migration of tumor cells in het TME/NRes, but it was exacerbated in PDT-treated het TME/Res.…”

Section: Discussionmentioning

confidence: 41%

Contribution of resident and recruited macrophages to the photodynamic intervention of colorectal tumor microenvironment

et al. 2015

View full text Add to dashboard Cite

The study of cellular interactions in the tumor microenvironment has become one of the main areas of research in the fight against cancer. Tumor-associated macrophages (TAMs) influence tumor progression and therapy response due to its functional plasticity. Regarding cancer treatment, photodynamic therapy (PDT) is a minimally invasive and clinically approved procedure that involves the administration of a photosensitizer (PS), a nontoxic photosensitizing drug which is selectively retained in neoplastic tissue. Here, we investigated the role of resident and nonresident macrophages in the context of a PDT-treated colorectal tumor by developing a combination of 2-D and three-dimensional (3-D) experimental platform, recreating tumor-stroma interactions in vitro. Enhancement of cytotoxicity of PDT was achieved in the presence of nonresident macrophages which had a strong anti-tumor phenotype mediated by the production of nitric oxide, IL-6, and tumor necrosis factor alpha (TNF-α). On the contrary, tumor resident macrophages induced a pro-tumor phenotype promoting tumor cell migration and endothelial stimulation. Due to their plasticity, tumor-resident or tumor-recruited macrophages can differentially influence the response of tumors to PDT, so their multifactorial roles should be considered in the overall design of anti-tumor therapeutic.

show abstract

“…We thus obtained an operating time acceptable for the range of volumes injected. Several studies reported a fluence value of 25 J/cm² to study PDT effect with 5-ALA [24][25][26] on GBM cells [27], and a photodynamic effect is expected to be observed at least within 5 mm of the cavity margin. Additionally, the proposed protocol relies on 2 W of power being emitted by the 70 mm cylindrical diffuser.…”

Section: Discussionmentioning

confidence: 99%

“…With this constraint, we computed the lighting duration to reach these fluence values for each balloon volume. A transfer function was defined to determine the lighting duration necessary to obtain a fluence value of 25 J/cm² [24][25][26][27] at a distance of 5 mm from the balloon wall depending on the volume of diffusing solution injected into the balloon (see figure 7). (Table 1).…”

Section: (Equation 5)mentioning

confidence: 99%

A novel device for intraoperative photodynamic therapy dedicated to glioblastoma treatment

et al. 2017

View full text Add to dashboard Cite

Aim: Photodynamic therapy (PDT) appears to be a valuable new treatment modality for cancer therapy. Studies have reported successful application of PDT for glioblastoma. Here, we introduce a new device dedicated to intraoperative PDT delivered early after fluoro-guided resection combined with a transfer function that determines the treatment time based on the size of the surgical resection cavity. Materials & methods: First, we describe the device, which is composed of a trocar, a balloon filled with a diffusing solution, and a fiber guide in which a cylindrical light diffuser is inserted. Ex vivo experiments were performed to measure the fluence rate inside biological tissues. A calibration factor was defined to convert power measurements into fluence rate values. Calf brains were used to simulate light propagation in human brain tissue, and the photosensitizer administration effect on optical properties was discussed. The temperature elevation during illumination was evaluated. Results: Light power was measured in tissues surrounding the device during ex vivo experiments. Using the previously characterized calibration factor, power measurements were converted to fluence rate values to obtain the transfer function. No thermal elevation was observed during a 2-h temperature test, and the impact of protoporphyrin IX on brain optical properties was considered negligible. Conclusion: A discussion of experimental precision is presented. The light duration determined by the abacus had a standard deviation of <1 min. This value is weak compared with the total illumination time necessary to treat one patient. The main advantage of our device lies in its straightforward implementation of intraoperative PDT for neurosurgery with acceptable dosimetry and easy treatment time.

show abstract

Effects of ALA-mediated photodynamic therapy on the invasiveness of human glioma cells

Abstract: Measurement of cell survival, and results from cultures with blocked cell proliferation, indicated a direct migratory inhibition effect on the invading cells rather than cytotoxicity as the most likely mechanism for the inhibition of invasiveness observed following ALA-mediated PDT.

Cited by 29 publications

References 34 publications

Matrigel: From discovery and ECM mimicry to assays and models for cancer research

Matrigel: From discovery and ECM mimicry to assays and models for cancer research

Contribution of resident and recruited macrophages to the photodynamic intervention of colorectal tumor microenvironment

A novel device for intraoperative photodynamic therapy dedicated to glioblastoma treatment

Contact Info

Product

Resources

About