Effects of Aldehyde Carbon Chain Length and Type of Luciferase on the Quantum Yields of Bacterial Bioluminescence

Lee, John; Murphy, Charles L.

doi:10.1007/978-1-4757-0638-3_28

Cited by 5 publications

(1 citation statement)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, there is direct evidence that under certain conditions luciferase can utilize flavin semiquinone as the substrate to produce light (Kurfurst et al, 1983). The possible role of semiquinones in the catalysis has been discussed previously (Eley et al, 1970;Lee and Murphy, 1973;Kosower, 1980;Puget and Michelson, 1972).…”

Section: Discussionmentioning

confidence: 86%

Bioluminescence decay kinetics in the reaction of bacterial luciferase with different aldehydes

Ismailov

Sobolev

Danilov

1990

J. Biolumin. Chemilumin.

View full text Add to dashboard Cite

At 22 degrees C the bioluminescence decay kinetics in the in vitro reaction catalysed by Vibrio harveyi luciferase in the presence of different aldehydes--nonanal, decanal, tridecanal and tetradecanal did not follow the simple exponential pattern and could be fitted to a two-exponential process. One more principal distinction from the first-order kinetics is the dependence of the parameters on aldehyde concentration. The complex bioluminescence decay kinetics are interpreted in terms of a scheme, where bacterial luciferase is able to perform multiple turnovers using different flavin species to produce light. The initial phase of the bioluminescent reaction appears to proceed mainly with fully reduced flavin as the substrate while the final one results from the involvement of flavin semiquinone in the catalytic cycle.

show abstract

Section: Discussionmentioning

confidence: 86%