Effects of Alexandrium minutum exposure on nutrition-related processes and reproductive output in oysters Crassostrea gigas

Haberkorn, Hansy; Lambert, Christophe; Goïc, Nelly Le; Moal, Jeanne; Suquet, Marc; Guéguen, Marielle; Sunila, Inke; Soudant, Philippe

doi:10.1016/j.hal.2010.01.003

Cited by 85 publications

(82 citation statements)

References 67 publications

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“…Exposed oysters contained 14.5 ± 11.2 µg eq STX g −1 (mean ± CI). Such individual variability is consistent with previous experiments in our laboratory (Haberkorn et al 2010(Haberkorn et al , 2011.…”

Section: Algal Exposuresupporting

confidence: 93%

“…In this study, viability of sperm from oysters exposed to A. catenella was not affected. In a similar experiment, the percentage of dead sperm from A. minutum-exposed Pacific oyster broodstock was significantly reduced compared with control conditions (Haberkorn et al, 2010).…”

Section: Viabilitymentioning

confidence: 85%

“…Alterations induced by noxious compounds have been observed in oyster gametes (Nice 2005). Pacific oyster (Crassostrea gigas) sperm exposed to Alexandrium minutum also showed a lower motility and ATP content, compared with unexposed cells (Haberkorn et al 2010). More recently, Heterocapsa circularisquama has been shown to reduce activity rate and swimming velocity of spermatozoa of Japanese pearl oyster, Pinctada fucata martensi (Basti et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…A number of sperm characteristics related to fertilizing capacity (viability, acrosomal integrity, mitochondrial function, DNA/chromatin integrity and ROS production) can be accurately and rapidly measured using flow cytometry coupled with fluorescent markers (Cordelli et al 2005, Gillan et al 2005. These measurements have mainly been developed in vertebrates, but very few attempts have been made to apply these methods to the assessment of sperm in bivalves (Adams et al 2003;PaniaguaChavez et al 2006;Favret et al 2010;Haberkorn 2010).…”

Section: Introductionmentioning

confidence: 99%

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Impact of the toxic dinoflagellateAlexandrium catenellaon Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa

Goïc

Hégaret

Fabioux

et al. 2013

Aquat. Living Resour.

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-The toxic dinoflagellate Alexandrium catenella recurrently blooms on the coasts of France and produces Paralytic Shellfish Toxins (PSTs) that accumulate in bivalves. These toxins can affect various physiological functions including reproduction. The present study aims to validate measurements of sperm viability, DNA content and mitochondrial membrane potential in Pacific oyster Crassostrea gigas using flow cytometry coupled with fluorescent markers, and to use these measurements to assess the cellular parameters of sperm from Pacific oysters exposed to A. catenella. These parameters may influence fertilization, embryogenesis and larval development in free-spawning shellfish. Sperm viability and DNA content estimation were assessed using SYBR-14, which only penetrates cells with intact membranes. Cell mortality was measured with propidium iodide (PI), which penetrates cells with membrane damage. Mitochondrial membrane potential, used as an estimate of mitochondrial function, was measured using JC-1 dye, which selectively enters into mitochondria and reversibly changes colour from green to orange as the membrane potential increases. To assess the effect of toxic algae on oyster sperm, broodstock (ripe oysters) were fed toxic (A. catenella) or non toxic (Heterocapsa triquetra) dinoflagellates at 250 cell ml −1 for 9 days. After this exposure period, mature oysters were stripped and cellular responses of sperm analysed. Average DNA staining, as measured by SYBR-14, appeared lower and more variable in gametes from A. catenella-exposed oysters than in those from control oysters fed H. triquetra. Additionally, mitochondrial membrane potential of sperm from A. catenella-exposed oysters was significantly higher (1.5 fold) than that of sperm produced by oysters fed H. triquetra. Both the increase of mitochondrial membrane potential and the modification of DNA structure can be expected to impact spermatozoa ability to fertilize oocytes and could thus impact related reproductive processes.

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Section: Algal Exposuresupporting

confidence: 93%

Section: Viabilitymentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impact of the toxic dinoflagellateAlexandrium catenellaon Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa

Goïc

Hégaret

Fabioux

et al. 2013

Aquat. Living Resour.

Self Cite

View full text Add to dashboard Cite

show abstract

“…These factors may also explain the pathology observed in the Saccostrea glomerata specimens. Indeed, the histological changes associated with the presence of PST-producing Alexandrium spp., which include haemocyte accumulation in the connective tissue surrounding the gut and digestive tubules (Haberkorn et al 2010b), were also observed in the Woolooware Bay oysters.…”

Section: Discussionmentioning

confidence: 72%