Effects of alfalfa flavonoids on broiler performance, meat quality, and gene expression

Abstract

Two hundred and forty 1-d-old Arbor Acre female broilers were used to study the effects of alfalfa flavonoids (AF) on broiler performance, meat quality, and gene expression. Chicken were fed with basal diet supplemented with AF at 0, 5, 10, or 15 mg kg −1 diet for a period of 42 d. Growth performance, meat quality, antioxidant effect and lipoprotein lipase (LPL), adipose triglyceride lipase (ATGL), peroxisome proliferator-activated receptor γ (PPARγ), and fatty acid synthase (FAS) gene expressions were investigated. Results showed that AF inclusion in the diet enhanced the body weight (BW) at 42 d of age and the average daily gain from 0 to 42 d, decreased the total cholesterol (TC) and low-density lipoprotein cholesterol (LDL) levels and increased HDL level in the serum, enhanced the superoxide dismutase (SOD), catalase (CAT), total antioxidant capacity (T-AOC), and glutathione peroxidase (GSH-Px), and decreased the malondialdehyde (MDA) concentrations in the serum. Also, AF supplementation decreased the abdomen fat rate, marble, and the drip loss and storage loss after the storage for 96 h. Gene expressions' results showed that AF inclusion decreased the FAS expression and increased the LPL, PPARγ, and ATGL expressions in the liver and adipose tissues, especially when the AF inclusion level was 15 mg kg −1 diet. These results indicate that AF were found to be effective for average daily gain and breast percentage promoting, meat quality and antioxidant activity improvement via upregulating the LPL, ATGL, PPARγ, and downregulating the FAS expression in adipose and liver tissues.

Antioxidant status, meat oxidative stability and quality characteristics of lambs fed with hesperidin, naringin or α‐tocopheryl acetate supplemented diets

“…In contrast, water holding of chicken meat was improved in a dose‐dependent manner after inclusion of hesperidin (20 mg kg −1 ) in the diet of broilers . Drip loss was also decreased after dietary inclusion (5–15 mg kg −1 ) of alfalfa flavonoids in broiler meat stored for 96 h at 4 °C …”

“…19 In contrast, water holding of chicken meat was improved in a dose-dependent manner after inclusion of hesperidin (20 mg kg −1 ) in the diet of broilers. 32 Drip loss was also decreased after dietary inclusion (5-15 mg kg −1 ) of alfalfa flavonoids in broiler meat stored for 96 h at 4 ∘ C. 22 The exact reasons for the discrepancies observed in the literature concerning growth performance parameters and meat quality traits are speculative, but they could be attributable to the different types and levels of flavonoids, periods of supplementation and animal species. Moreover, the variability in the efficacy of flavonoids on growth performance could also be a result, among others, of the basal diet composition, feed intake level, hygienic standards and environmental conditions.…”

“…However, dietary supplementation with flavonoids from the plant Allium mongolicum Regel (11–33 mg kg −1 ) significantly increased the daily weight gain and average daily feed intake in sheep . Alfalfa flavonoid dietary inclusion (5–15 mg kg −1 ) also enhanced the final body weight (42 days) and the average daily gain in broilers; however, this effect was not observed after their addition (0.4–1.2 g kg −1 ) in the diet of rabbits . Inclusion of hesperidin (1.5–3 g kg −1 ) or naringin (0.75–1.5 g kg −1 ) or a mixture of quercetin and rutin (0.25–1 g kg −1 ) or soy isoflavone (20 mg kg −1 ) in the diet did not affect the feed intake, body weight and carcass traits in broilers.…”

PENGARUH TINGKAT PEMBERIAN EKSTRAK DAUN BURAHOL (Stelechocarpus burahol) DALAM RANSUM TERHADAP BOBOT ORGAN DALAM AYAM BROILER

“…Ekstrak daun burahol mengandung senyawa flavonoid meliputi auron, flavanon dan flavanol yang dapat digunakan sebagai antibakteri (Hidayat et al, 2011). Menurut Ouyang et al, (2016) flavonoid dapat meningkatkan ekspresi insulin-like growth factor (IGF1) yang berperan sebagai mediator dalam proliferasi fibroblas dan sintesis kolagen, sehingga memicu pertumbuhan masa otot.…”

Investigation of Chemical Composition, Antioxidant Activity, and the Effects of Alfalfa Flavonoids on Growth Performance

“…In vitro tests showed that the flavonoids activated the total and isozymatic activities of SOD, POD, and CAT. Ouyang et al [20] had found that alfalfa flavonoids enhanced the in vivo antioxidant activities (such as SOD, CAT, T-AOC, and GSH-PX) and decreased the level of MDA in serum. In addition, the average daily gain and body weight of the broilers fed with flavonoids were higher than those of broilers fed with basal diet.…”