Effects of Allium sativum and Allium cepa on semen characteristics, sperm reserves and haematology of rabbit bucks

Abstract

The effect of dietary inclusion of garlic and onion on semen characteristics, gonadal, extragonadal sperm reserves and haematology of rabbit bucks were evaluated. Twenty-four rabbit bucks of average age and weight 10 ± 2.0 months and 1.47 ± 0.01 kg respectively, were used for the study. They were randomly assigned into four groups of six bucks each. Group A served as control, while Groups B, C and D received dietary inclusion of 5% garlic, 5% onion, 2.5% garlic + 2.5% onion, respectively. The fresh bulbs of garlic and onions were peeled, air-dried, and the dried bulbs were weighed, added to the feed and grounded together to form experimental diets. Before dietary supplementation, semen samples were collected to serve as baseline values, followed by weekly collections for another 9 weeks using an artificial vagina. Haematological parameters were examined according to the standard procedure, while testosterone profile was conducted using the ELISA method. At the termination of the experiment, two bucks from each group were euthanised, and the testes were harvested to evaluate gonadal and extragonadal sperm reserves. No significant (p > 0.05) difference was recorded in the live weight, sperm concentration, and sperm abnormality of the rabbit bucks. A significant (p < 0.05) difference was observed in the ejaculate volume, gross motility, pH, reaction time, and percentage of live spermatozoa. The epididymal sperm reserves in group B, was significantly (p < 0.05) higher in the right than left. Testosterone profile showed significant (p < 0.05) difference at 9 and 10 a.m. On haematology, there was a significant increase in PCV, RBC count, haemoglobin concentration and WBC count in groups B, C and D by week 9 compared to the control. In conclusion, the dietary inclusion of garlic and onion effectively improved the spermiogram of rabbit bucks.