Effects of Aloe vera extract on growth, viability, ultrastructure and expression of mRNA for antioxidant enzymes in bovine secondary follicles cultured in vitro

Azevedo, Venância Antônia Nunes; Barroso, P.A.A.; Vasconcelos, Erlândia Márcia; Costa, Francisco das Chagas; Assis, Ernando Igo Teixeira de; Silva, Bianca R.; Paulino, Laís R.M.; Silva, Anderson Weiny Barbalho; Donato, Mariana M.A.; Peixoto, Christina A.; Silva, José R.V.; Souza, Ana Liza Paz

doi:10.1016/j.anireprosci.2022.107078

Cited by 5 publications

(2 citation statements)

References 43 publications

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“…The antioxidant system that can eliminate reactive oxygen. For example, antioxidants can be synthesized within germ cells, such as superoxide dismutase (SOD) (20) and Glutathione peroxidase 1 (GPX1) (21), which can decompose ROS into non-toxic products. Excessive production of ROS can lead to oxidative stress and damage to oocytes, which may impair their maturation.…”

Section: Discussionmentioning

confidence: 99%

CX43 and oxidative stress are the targets of BCB staining to predict the developmental potential of buffalo oocytes

Yang

Duan

et al. 2023

Preprint

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Background Brilliant cresyl blue (BCB) staining can stain oocytes and differentiated oocytes will lead to different developmental outcomes. This technique has been studied in multiple species, but it is still unclear whether buffalo oocytes can be used for developmental potential prediction through BCB staining methods. This study used the BCB staining method to group buffalo oocytes (BCB + and BCB-) and perform in vitro maturation, in vitro fertilization, and embryo culture. By statistical analysis, the effect of BCB staining on predicting the developmental potential of buffalo oocytes will be explored. At the same time, molecular biology techniques will be used to detect gap junction protein and oxidative stress-related indicators to explore the molecular mechanism of BCB staining predicting oocyte cell developmental potential. Methods The oocytes were divided into BCB + and BCB- groups using BCB staining technique. And then mainly uses in vitro maturation, in vitro fertilization and embryo culture techniques of buffalo oocytes to analyze their developmental potential, and uses immunofluorescence staining to detect the expression level of CX43 protein, DCFH-DA probe staining to detect ROS levels, and qPCR to detect the expression levels of the antioxidant related genes SOD2 and GPX1. Results Our results showed that the in vitro maturation rate, embryo cleavage rate, and blastocyst rate of buffalo oocytes in the BCB + group were significantly higher than those in the BCB- group and the control group (P < 0.05). The expression level of CX43 protein in the BCB + group was higher than that in the BCB- group both before and after maturation (P < 0.05). The intensity of ROS in the BCB + group was significantly lower than that in the BCB- group (P < 0.05), and the expression levels of the antioxidant-related genes SOD2 and GPX1 in the BCB + group were significantly higher than those in the BCB- group (P < 0.05). Conclusions BCB staining can effectively predict the developmental potential of buffalo oocytes. The results of BCB staining are positively correlated with the expression of gap junction protein and antioxidant-related genes and negatively correlated with the ROS level, suggesting that the mechanism of BCB staining in predicting the developmental potential of buffalo oocytes may be closely related to antioxidant activity.

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Section: Discussionmentioning

confidence: 99%

CX43 and oxidative stress are the targets of BCB staining to predict the developmental potential of buffalo oocytes

Yang

Duan

et al. 2023

Preprint

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“…According to the results of the study, the volume of mitochondria occupied by lipid droplets was higher (P<0.03) in oocytes from regressing follicles (193.0±10.4/1000microns 3 and 3.5±0.7%) than in the growth stage and in the preovulatory stage (118.7±14.4/ 1000microns 3 and 1.1±0.3%; 150.5±28.7/1000microns 3 and 1.6±0.2%, respectively). Thus, it was found that lipid droplets undergo spatial reorganization, i.e., from peripheral to uniform distribution during the growth phase and the mitochondrial lipid contact area increases as the follicle matures (Dadarwal et al 2015;Fayezi et al 2018;Mylostyvyi et al 2021;Azevedo et al 2022;Nascimento et al 2022).…”

Section: Discussionmentioning

confidence: 99%

Results of Ultrasound Studies of the Growth Dynamics of Dominant, Subdominant Follicles and Determination of Estradiol Concentration in the Preovulatory Period in Cows

2023

Int J Vet Sci

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The mechanism of natural selection of the dominant follicle and hormonal regulation of this process in cattle is still insufficiently studied. The purpose of the study was to study the dynamics of the growth of subdominant and dominant follicles in Holstein cows and optimize the technique of scanning the ovaries during the estrus cycle, measuring the parameters of follicle growth, analyzing the results of sonograms and determining the concentration of estradiol in the pre-ovulatory period. To study the growth dynamics of subdominant and dominant follicles, the method of scanning the ovaries of cows during the estrous cycle using PU2200Vet devices and the Mindray Z5 Vet ultrasound scanner equipped with a convex endorectal sensor with a frequency of 5.0-7.0MHz was used. Two or three waves of dominant follicle growth with a duration of 4 to 12 days were detected in the studied animals. The maximum area of dominant follicles ranged from 191.18 to 243.18mm 2 . It is proved that the growth of dominant follicles in cows during the spontaneous estrous cycle is accompanied by an increase in the subdominant follicle population, per dominant follicle corresponds from 13.66 to 21.0 subdominant follicle. According to the results of the study, the growth rate of dominant follicle was higher compared to the growth of subdominant follicle, the growth rate of dominant follicle was from 0.9 to 4.43mm/day, this indicator for subdominant follicle was from 0.26 to 1.40mm/day. In the pre-ovulatory period, cows have a decrease in the amount of estradiol in the blood serum. The use of Doppler ultrasound scanning of the blood vessels of the corpus luteum allows determining the functional activity of the corpus luteum of the estrous cycle.

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Ag/TiNPS nanocatalyst: biosynthesis, characterization and photocatalytic activity

Muraro

Wouters

Pavoski

et al. 2023

Journal of Photochemistry and Photobiology A: Chemistry

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Effects of Aloe vera extract on growth, viability, ultrastructure and expression of mRNA for antioxidant enzymes in bovine secondary follicles cultured in vitro

Cited by 5 publications

References 43 publications

CX43 and oxidative stress are the targets of BCB staining to predict the developmental potential of buffalo oocytes

CX43 and oxidative stress are the targets of BCB staining to predict the developmental potential of buffalo oocytes

Results of Ultrasound Studies of the Growth Dynamics of Dominant, Subdominant Follicles and Determination of Estradiol Concentration in the Preovulatory Period in Cows

Ag/TiNPS nanocatalyst: biosynthesis, characterization and photocatalytic activity

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