Effects of altered extracellular and intracellular calcium concentration on hyperpolarizing responses of the hamster egg.

Igusa, Yukio; Miyazaki, Shunichi

doi:10.1113/jphysiol.1983.sp014783

Cited by 237 publications

(192 citation statements)

References 30 publications

Supporting

Mentioning

181

Contrasting

Order By: Relevance

“…Fig. 1B shows that 1 s current pulses of 0.5-~ nA Ca t* into unfertilized control (untreated) hamster eggs caused HRs [12,21]. As has been found prev=ously [12], a threshold mjecuon current of 2.19 ± 0.5 nA (mean and SD) triggered an HR in 10/15 eggs that was regenerative [12].…”

Section: Rf:~ 'Jltsmentioning

confidence: 56%

“…ly [12,18] Eggs were bathed m M2 plus 4 mg/ml bovine serum albumin or else in a CaJ).free manganese-containing soluuon [12] Thlmerosal (sodium eth)lmercurnhlosahcylate), and other sulfhydryl reagents were dissolved d~rectly into the appropriate med~a lnos~tol 1,4,5-tnsphosphate was dissolved at I mM m a KCl buffer [18] Chemlcals were from Sigma Chemlcals (Poole, UK)…”

Section: Methodsmentioning

confidence: 99%

“…tions [12]. To assay the sens~tw~ty of CICR m control and thimerosal-treated eggs, Ca z+ was rejected mntophoretically.…”

Section: Rf:~ 'Jltsmentioning

confidence: 99%

“…CICR has been demonstrated to occur in unfertilized hamster eggs [12]. The sensiuwty of CICR in hamster eggs increases 10-fold after sperm-egg fuston and th~s leads to a series of transient oscillations ,n [Ca""], [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…The sensiuwty of CICR in hamster eggs increases 10-fold after sperm-egg fuston and th~s leads to a series of transient oscillations ,n [Ca""], [12][13][14]. These fertlhzation-tnduced [Ca2~'], oscfllatmns Correspondence address K [13,14].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Thimerosal causes calcium oscillations and sensitizes calcium‐induced calcium release in unfertilized hamster eggs

Swann

1991

FEBS Letters

129

View full text Add to dashboard Cite

Calcium‐induced‐calcium‐release (CICR) was assayed in unfertilized golden hamster eggs by injecting Ca2+ and monitoring Ca2+‐dependent hyper‐polarizing responses (HRs) and Ca2+‐sensitive fluo‐3 fluorescence. Incubating eggs in the sulfhydryl reagent thimerosal caused [Ca2+]1 oscillations as monitored by Ca2+‐dependent HRs and decreased approximately 10‐fold the Ca2+ injection current required to generate an HR and cause a large intracellular Ca2+ increase. Thimerosal also enhanced the sensitivity of eggs to Ca2+ injection in a calcium‐free medium. The effects of thimerosal on CICR were prevented by dithiothreitol and were not mimicked by injecting inositol 1,4,5‐triphosphate. The data suggest that thimerosal may be an alternative agent for studying CICR in caffeine‐insensitive cells.

show abstract

Section: Rf:~ 'Jltsmentioning

confidence: 56%

Section: Methodsmentioning

confidence: 99%

“…tions [12]. To assay the sens~tw~ty of CICR m control and thimerosal-treated eggs, Ca z+ was rejected mntophoretically.…”

Section: Rf:~ 'Jltsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Thimerosal causes calcium oscillations and sensitizes calcium‐induced calcium release in unfertilized hamster eggs

Swann

1991

FEBS Letters

129

View full text Add to dashboard Cite

show abstract

The ins and outs of meiosis

1999

View full text Add to dashboard Cite

During oogenesis the oocyte is arrested in meiosis twice. First at prophase I, then a second time at metaphase I in many invertebrates and in metaphase II in the vast majority of vertebrates. Meiosis resumption is triggered by the sperm. This article examines mechanisms that cause oocytes' arrest in meiosis and how spermatozoa help the oocyte to get out of this cellular predicament. J. Exp. Zool. (Mol. Dev. Evol.) 285:226–236, 1999. © 1999 Wiley‐Liss, Inc.

show abstract

Mechanism of Ca2+ release at fertilization in mammals

Swann

Parrington

1999

J. Exp. Zool.

View full text Add to dashboard Cite

At fertilization in mammals the sperm triggers a series of oscillations in intracellular Ca2+ within the egg. These Ca2+ oscillations activate the development of the egg into an embryo. It is not known how the sperm triggers these Ca2+ oscillations. There are currently three different theories for Ca2+ signaling in eggs at fertilization. One idea is that the sperm acts as a conduit for Ca2+ entry into the egg after membrane fusion. Another idea is that the sperm acts upon plasma membrane receptors to stimulate a phospholipase C (PLC) within the egg which generates inositol 1,4,5‐trisphosphate (InsP3). We present a third idea that the sperm causes Ca2+ release by introducing a soluble protein factor into the egg after gamete membrane fusion. In mammals this sperm factor is also referred to as an oscillogen because, after microinjection, the factor causes sustained Ca2+ oscillations in eggs. Our recent data in sea urchin egg homogenates and intact eggs suggests that this sperm factor has phospholipase C activity that leads to the generation of InsP3. We then present a new version of the soluble sperm factor theory of signaling at fertilization. J. Exp. Zool. (Mol. Dev. Evol.) 285:267–275, 1999. © 1999 Wiley‐Liss, Inc.

show abstract

Effects of altered extracellular and intracellular calcium concentration on hyperpolarizing responses of the hamster egg.

Cited by 237 publications

References 30 publications

Thimerosal causes calcium oscillations and sensitizes calcium‐induced calcium release in unfertilized hamster eggs

Thimerosal causes calcium oscillations and sensitizes calcium‐induced calcium release in unfertilized hamster eggs

The ins and outs of meiosis

Mechanism of Ca2+ release at fertilization in mammals

Contact Info

Product

Resources

About