1999
DOI: 10.1159/000028313
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Effects of Ambroxol on Spontaneous or Stimulated Generation of Reactive Oxygen Species by Bronchoalveolar Lavage Cells Harvested from Patients With or Without Chronic Obstructive Pulmonary Diseases

Abstract: We examined the effects of ambroxol on spontaneous or stimulated generation of reactive oxygen species (ROS) by bronchoalveolar lavage (BAL) cells prepared from 6 patients with chronic obstructive pulmonary disease (COPD) and age-matched control subjects without COPD. The ROS produced by BAL cells were measured by the lucigenin-dependent chemiluminescence method. The application of ambroxol into culture media containing BAL cells inhibited spontaneous and stimulated generation of ROS by BAL cells harvested fro… Show more

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Cited by 13 publications
(5 citation statements)
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“…In the animal studies, treatment with anti-oxidant reagents or genetic manipulation, the over-expression of Cu/Zn superoxide dismutase or deficiency of nuclear erythroid 2 p45-related factor 2, a transcription factor regulating many antioxidant genes, were shown to be protective in the airways ( 37 38 39 ). Ambroxol has been shown to have anti-oxidant properties and reduce oxidant production from inflammatory cells In vitro , such as polymorphonuclear cells, alveolar macrophages, or human bronchial epithelial cells ( 16 40 41 42 ), and inhibited AHR induced by ozone in dogs ( 43 ). In the present study, we demonstrated that ambroxol treatment begun after OVA challenge of sensitized mice decreased the levels of protein carbonyls in BAL fluid, a biomarker of oxidative stress ( 44 ).…”
Section: Discussionmentioning
confidence: 99%
“…In the animal studies, treatment with anti-oxidant reagents or genetic manipulation, the over-expression of Cu/Zn superoxide dismutase or deficiency of nuclear erythroid 2 p45-related factor 2, a transcription factor regulating many antioxidant genes, were shown to be protective in the airways ( 37 38 39 ). Ambroxol has been shown to have anti-oxidant properties and reduce oxidant production from inflammatory cells In vitro , such as polymorphonuclear cells, alveolar macrophages, or human bronchial epithelial cells ( 16 40 41 42 ), and inhibited AHR induced by ozone in dogs ( 43 ). In the present study, we demonstrated that ambroxol treatment begun after OVA challenge of sensitized mice decreased the levels of protein carbonyls in BAL fluid, a biomarker of oxidative stress ( 44 ).…”
Section: Discussionmentioning
confidence: 99%
“…Both drugs are also approved for veterinary administration and commonly marketed as tablets, granules, syrups, capsules, drops and injections for use in the therapy of pulmonary diseases, chronic bronchitis or pneumonia. Other clinically significant properties of ambroxol are radical scavenger activity [1,2], in vivo inhibition of doxorubidn-induced lipid peroxidation [2] and blocking generation of reactive oxygen species in placenta [3] and bronchoalveolar cells [4]. It also prevents Helicobacter pylori associated gastric carcinogenesis [1] and has valuable antiinflammatory activity by inhibiting the release of inflammatory mediators from human lenkocytes and mast cells [5].…”
Section: Introductionmentioning
confidence: 99%
“…Ambroxol and beclomethasone alone showed an inhibitory effect on LPS-induced IL-8 and MPO release and 3-NT formation in BEAS-2B cells cocultured with neutrophils, and the combination of the two drugs enhanced their effect on IL-8 and 3-NT. In other studies, ambroxol or beclomethasone were able to inhibit nitrosative stress [8,13,22], and ambroxol alone showed a great antioxidant activity, operating as scavenger of HClO and OH, and also revealed the capacity to decompose H 2 O 2 in an in vitro study [12], but the combination has not previously been investigated in this context.…”
Section: Discussionmentioning
confidence: 99%
“…Ambroxol has been shown to prevent generation of reactive oxygen species by broncho-alveolar lavage cells from COPD patients [13] suggesting that it may be useful in the management of COPD [14,15]. Moreover, ambroxol's antioxidant properties may be of clinical benefit in virus-induced infections.…”
Section: Introductionmentioning
confidence: 99%