Effects of ammonium on the antioxidative response in Hydrilla verticillata (L.f.) Royle plants

Wang, Chao; Zhang, Song He; Wang, Pei Fang; Li, Wei; Lu, Jie

doi:10.1016/j.ecoenv.2009.08.012

Cited by 84 publications

(45 citation statements)

References 48 publications

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“…2). These are consistent with previous reports (Wang et al, 2009). In addition, high NH4 + concentration decreased soluble protein content, likely due to ROS-dependent protein degradation or fragmentation (John et al, 2008).…”

Section: Discussionsupporting

confidence: 83%

Physiological Response and Comparative Proteomic Analysis of Tobacco Seedling Roots to NH4+

Tan¹

2017

IJAB

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Plants absorb nitrogen, an essential element for their growth and development, mainly in the form of nitrate or ammonium. These nitrogen species have different effects on crops, little is known about their precise influence at both physiological and proteomic levels. This study investigated these mechanisms, hydroponically grown tobacco seedlings treated with three different ratios of NO3 -to NH4 + (10:0, 5:5 or 0:10) for 15 days. The results showed that exposure to high NH4 + concentration affected root morphology and inhibited root development. Moreover, malondialdehyde content and the activity of glutamine synthetase, superoxide dismutase, peroxidase and glutathione S-transferase increased with the concentration of NH4 + in solution. Two-dimensional protein gel analysis showed that 40 protein were differentially expressed between the three treatments. 36 of these proteins were identified using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). Gene ontology analysis indicated that these proteins were involved in metabolism, energy production, cell defense or signal transduction or structural constituents related pathways. Our results suggest that tobacco seedling roots are subjected to NH4 + toxicity that triggers oxidative stress. Our results help understanding the responses to different nitrogen species and to NH4 + toxicity in roots.

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Section: Discussionsupporting

confidence: 83%

Physiological Response and Comparative Proteomic Analysis of Tobacco Seedling Roots to NH4+

Tan¹

2017

IJAB

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“…Most scientists agree that phytoplankton shading promoted by increasing inputs of phosphorus is an underlying cause of the decreasing abundance, but the role of nitrogen has received increasing attention in recent years (Moss, 2001;Jeppesen et al, 2007;Moss et al, 2013). In previous studies, two main mechanisms have been identified to explain the effects of nitrogen on submersed macrophytes: 1) it promotes the growth and hence shading of phytoplankton (Sayer et al, 2010a(Sayer et al, , 2010b or periphyton (Olsen et al, 2015;Zhao et al, 2016) that uses nitrogen as its nutrient source; 2) it causes physiological stress on the submersed macrophytes and limits the metabolism of carbohydrates (Cao et al, 2009a;Yuan et al, 2015;Gao et al, 2015), inducing oxidative stress (Wang et al, 2010;Zhang et al, 2011) and inhibiting photosynthesis (Wang et al, 2008;Su et al, 2012). Experimental studies have revealed that ammonium (NH 4 )-related physiological stress may be aggravated by low light conditions .…”

Section: Introductionmentioning

confidence: 99%

Does the responses of Vallisneria natans (Lour.) Hara to high nitrogen loading differ between the summer high-growth season and the low-growth season?

Wang

et al. 2017

Science of The Total Environment

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“…submerged plants and uncontrollable increases in E. crassipes population (Dyhrman, 2008;Ripley et al, 2006;Wang, Zhang, Wang, Li, & Lu, 2010). The complexity of nutrient loading is matched by the complexity of nutritional strategies and capabilities of aquatic plants.…”

Section: Discussionmentioning

confidence: 99%

Identification of gene fragments related to nitrogen deficiency in Eichhornia crassipes (Pontederiaceae)

Jiang

et al. 2014

RBT

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Abstract:Eichhornia crassipes is an aquatic plant native to the Amazon River Basin. It has become a serious weed in freshwater habitats in rivers, lakes and reservoirs both in tropical and warm temperate areas worldwide. Some research has stated that it can be used for water phytoremediation, due to its strong assimilation of nitrogen and phosphorus, and the accumulation of heavy metals, and its growth and spread may play an important role in environmental ecology. In order to explore the molecular mechanism of E. crassipes to responses to nitrogen deficiency, we constructed forward and reversed subtracted cDNA libraries for E. crassipes roots under nitrogen deficient condition using a suppressive subtractive hybridization (SSH) method. The forward subtraction included 2 100 clones, and the reversed included 2 650 clones. One thousand clones were randomly selected from each library for sequencing. About 737 (527 unigenes) clones from the forward library and 757 (483 unigenes) clones from the reversed library were informative. Sequence BlastX analysis showed that there were more transporters and adenosylhomocysteinase-like proteins in E. crassipes cultured in nitrogen deficient medium; while, those cultured in nitrogen replete medium had more proteins such as UBR4-like e3 ubiquitinprotein ligase and fasciclin-like arabinogalactan protein 8-like, as well as more cytoskeletal proteins, including actin and tubulin. Cluster of Orthologous Group (COG) analysis also demonstrated that in the forward library, the most ESTs were involved in coenzyme transportation and metabolism. In the reversed library, cytoskeletal ESTs were the most abundant. Gene Ontology (GO) analysis categories demonstrated that unigenes involved in binding, cellular process and electron carrier were the most differentially expressed unigenes between the forward and reversed libraries. All these results suggest that E. crassipes can respond to different nitrogen status by efficiently regulating and controlling some transporter gene expressions, certain metabolism processes, specific signal transduction pathways and cytoskeletal construction. Rev. Biol. Trop. 62 (4):

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Effects of ammonium on the antioxidative response in Hydrilla verticillata (L.f.) Royle plants

Cited by 84 publications

References 48 publications

Physiological Response and Comparative Proteomic Analysis of Tobacco Seedling Roots to NH4+

Physiological Response and Comparative Proteomic Analysis of Tobacco Seedling Roots to NH4+

Does the responses of Vallisneria natans (Lour.) Hara to high nitrogen loading differ between the summer high-growth season and the low-growth season?

Identification of gene fragments related to nitrogen deficiency in Eichhornia crassipes (Pontederiaceae)

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