Effects of Amniotic Membrane–Derived Fibroblast Supernatant on Corneal Epithelium

Abstract

Citation: Higa K, Higuchi J, Kimoto R, et al. Effects of amniotic membranederived fibroblast supernatant on corneal epithelium. Invest Ophthalmol Vis Sci. 2019;60:3718-3726. https:// doi.org/10.1167/iovs.19-27041 PURPOSE. To evaluate the effects of human amniotic membrane-derived fibroblast (AMF) cell supernatant (AMF-sup) on corneal epithelium.METHODS. The phenotype of AMF cells was analyzed by flow cytometry using cell-surface markers. AMF cells were also induced to form osteoblasts and neural cells, and cell phenotypes were observed by staining and RT-PCR. Cultivated human corneal limbal epithelial sheets generated using AMF-sup were analyzed using immunohistochemistry and colony-forming efficiency, and the wound healing of epithelial defects was observed using a tissue-punch method. The effects of instillation of each supernatant in a rabbit corneal epithelial wound healing model were compared. RESULTS.Mesenchymal stem cell (CD29, CD44, CD73, and CD90) and neural crest (CD49d and CD56) markers were expressed on the AMF cell surface. Following induction of differentiation, isolated AMF cells showed characteristics of osteoblasts and neural cells. Application of AMF-sup resulted in maintenance of the limbal epithelial phenotype and immature state, and significantly promoted wound healing in cultivated human corneal limbal epithelial sheets (P < 0.05) and rabbit corneal epithelium (P < 0.05) compared with the control.CONCLUSIONS. These data suggest that AMF cells have multi-differentiation potential, and that AMF-sup is effective in maintaining the limbal epithelial phenotype and promoting corneal epithelial wound healing, which may be of value in ocular surface reconstruction.

Wireless‐Powered Electrical Bandage Contact Lens for Facilitating Corneal Wound Healing

“…[ 7 , 8 , 9 , 10 ] Several clinical attempts have been developed to accelerate and improve wound healing, including the use of lubricants, autologous serum, drug‐elution bandage, bandage contact lens, and amniotic membrane transplantation. [ 10 , 11 , 12 ] However, applying gauze and bandage to the injured eye does not allow for direct observation of the corneal wound recovery and intraocular inflammatory reactions; besides, it affects the patient's aesthetic appearance. More importantly, most of these methods are passive treatments that rarely participate in controlling endogenous cell behaviors.…”