Effects of <i>Paljeong-san</i> Pharmacopuncture on Experimental Rat Model of Benign Prostatic Hyperplasia

Abstract

Objectives : The aim of this study is to evaluate the effect of Paljeong-san pharmacopuncture(PJS) on the rat model of benign prostatic hyperplasia(BPH). Methods : Rats were divided into 5 groups, with 6 rats in each group. The 5 groups included sham-operated group(sham group), BPH model group(BPH group), finasteride-treated group (fina group), PJS-treated groups(PJS 10 and PJS 100 group). Testosterone was injected subcutaneously to the castrated rats except sham group for BPH model. During 4-week experimental period, finasteride(5 mg/kg) was administrated orally once daily in fina group, PJS in PJS 10(10 mg/kg) and PJS 100(100 mg/kg) group and normal saline in sham and BPH group were injected subcutaneously once daily at Jungwan(CV12). We checked prostate weights, serum concentration of dihydrotestosterone(DHT), morphologic changes of the prostate, and the amount of expression of the proliferating cell nuclear antigen(PCNA) and 5α-reductase gene to evaluate the effect of PJS after 4-week experimental period. Results : 1. PJS and finasteride treatment reduced prostate weights comparing with BPH group, but PJS-treated groups showed no significant changes, unlikely fina group. 2. PJS-treated groups showed significant degreases in concentration of DHT. 3. PJS-treated groups showed significant degreases concentration-dependently in the amount of expression of the PCNA and 5α-reductase gene. 4. PJS treatment showed shrinking of thickness in the prostatic epithelial tissue. Conclusions : PJS has the effects that improve the symptoms of BPH through inhibiting proliferation of the prostatic tissues.

Corn silk extract improves benign prostatic hyperplasia in experimental rat model

“…In addition, the concentration of 5α-R2 was decreased in experimental groups treated with a Schisandrae Fructus extract although the decrease was not dose-dependent [ 16 ]. Furthermore, in an experiment to see the effects of Paljeong-san Pharmacopuncture on BPH, DHT concentrations were significantly decreased without dose dependency in rats with induced BPH compared to the results of the study's BPH control group [ 31 ].…”

“…Moreover, the CSE groups responded to CSE treatment in a dose-dependent manner, thereby suggesting that CSE may inhibit prostate enlargement via a mechanism that inhibits prostate cell proliferation. Previous studies have shown that the PCNA expression level is significantly increased in animal models with induced BPH, and that excessive cell proliferation in stroma or at the epithelial surface has a critical role in the development of BPH [ 29 30 31 32 33 34 ]. The present in vivo study is the first to demonstrate the effect of CSE treatment on mRNA expression of PCNA.…”

In vivo evaluation of hot water extract of Acorus gramineus root against benign prostatic hyperplasia

“…Finasteride was used as a positive control [20]. Quantitative PCR amplification was performed in triplicate using the QuantiTect Promer assay (QT00070518 for SRD5A2, QT00073451 for AR and QT01680476 for ACTB; Qiagen).…”