Effects of Amphotericin B on Renal Transport

Proton Permeability of Renal Membranes: Influence of Amphotericin B

“…An important parameter in this control is the proton permeability of the brush border membranes on the lumenal side of the renal proximal tubule [I], This report examines the relative permeability of renal membranes to ions and protons. The influence of ampho tericin B on these observations was examined due to its strong effects on renal membrane function.Renal brush border membrane vesicles were prepared from rabbit kidney cortex using a method of homogenation, differential centrifugation and Ca:+ precipitation [2,3]. The potential sensitive dye, 3,3-dipropylthiodicarbocyanine iodide [diS-C3-(5)] was used to measure the membrane potential.…”

“…The potential sensitive dye, 3,3-dipropylthiodicarbocyanine iodide [diS-C3-(5)] was used to measure the membrane potential. The relative ion permeabilities were derived from the observed membrane potential and the ionic concentrations in the buffer solutions using the constant field equation [2]. Vesicles were equilibrated in solutions of choline and gluconate salts buffered to pHs of 5.5-8.5.…”