Effects of Amyloid Precursor Protein Overexpression on NF-κB, Rho-GTPase and Pro-Apoptosis Bcl-2 Pathways in Neuronal Cells

Chan, Hong Hao; Leong, Yong Qi; Voon, Shee Man; Pan, Meng Liy; Leong, Chee Onn; Lim, Chooi Ling; Koh, Rhun Yian

doi:10.52547/rbmb.9.4.417

Cited by 5 publications

(2 citation statements)

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“…The development of AD is attributed to various factors, including the accumulation of the Aβ peptide, disturbances in glutamatergic neurotransmission, the cholinergic hypothesis, alterations in tau protein, and the impact of oxidative stress and calcium imbalance (1). Glial cellderived neurotrophic factor (GDNF), a neurotrophic factor most commonly found in the human brain, is mainly produced by glial cells such as microglia, astrocytes, and neurons (2). A reduction in GDNF levels has been linked to the emergence of AD symptoms and pathologies, indicating its potential as a therapeutic target for neurodegenerative diseases.…”

Section: Introductionmentioning

confidence: 99%

Effects of Aerobic Interval Training Along with Ginkgo biloba Supplementation on Passive Avoidance Memory and GDNF Gene Expression in Alzheimer Rats

Basirat Dehkordi,

Mogharnasi,

Vahidian-Rezazadeh

et al. 2024

Jundishapur J Nat Pharm Prod

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Background: Alzheimer's disease (AD) represents the most prevalent form of dementia, characterized by memory loss, and is a complex, progressive neurodegenerative condition. Objectives: This study aimed to explore the impact of aerobic interval training (AIT) combined with Ginkgo biloba (GB) supplementation on passive avoidance memory function and the expression of the glial cell-derived neurotrophic factor (GDNF) gene in rats afflicted with AD. Methods: A total of forty-four male Wistar rats, each weighing approximately 250 ± 20 g, were maintained in a controlled environment at a specified temperature. Over 8 weeks (5 days per week), they underwent AIT and received GB supplements at a dosage of 100 mg/kg. The rats were randomly assigned into six groups: healthy control (HC) with 7 rats, AD control (AC) with 8 rats, amyloid-β (Aβ) injection plus GB supplement (Aβ + GB) with 8 rats, Aβ injection plus GB supplement plus AIT (Aβ + GB + AIT) with 8 rats, Aβ injection plus AIT (Aβ + AIT) with 8 rats, and sham surgery (SS) with 5 rats. The shuttlebox apparatus was utilized to assess the effect of Aβ on passive avoidance memory and to confirm disease induction. GDNF gene expression was measured using the qRT-PCR method. Results: The study found significant improvements in passive avoidance memory in the Aβ + AIT and Aβ + GB + AIT groups compared to the AC group after 8 weeks of AIT and GB supplementation (P = 0.02 and P = 0.000, respectively). Long-term memory performance was notably enhanced in the Aβ + GB + AIT group (P = 0.000). Additionally, GDNF expression significantly increased in the Aβ + GB + AIT and Aβ + GB groups in comparison to the AC group (P = 0.000 and P = 0.01, respectively). Conclusions: A synergistic effect of AIT and GB supplementation was observed, leading to an increase in GDNF gene expression and improvements in both passive avoidance memory and long-term memory in male rats with AD.

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Section: Introductionmentioning

confidence: 99%

Effects of Aerobic Interval Training Along with Ginkgo biloba Supplementation on Passive Avoidance Memory and GDNF Gene Expression in Alzheimer Rats

Basirat Dehkordi,

Mogharnasi,

Vahidian-Rezazadeh

et al. 2024

Jundishapur J Nat Pharm Prod

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“…SOX2 expression is upregulated in immature Schwann cells after PNI event (16). NF-ĸB pathway is involved in many diseases and neuron regenerative process (17). Based on the literature, FAK and Src pathways are involved in Schwann cell proliferation and differentiation (18).…”

Section: Introductionmentioning

confidence: 99%

Melatonin Induced Schwann Cell Proliferation and Dedifferentiation Through NF-ĸB, FAKDependent but Src-Independent Pathways

Govindasamy

Chok

et al. 2022

rbmb.net

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Background: Peripheral nerve injury (PNI) is a common condition that compromises motor and sensory functions. Peripheral nerves are known to have regenerative capability and the pineal hormone, melatonin, is known to aid nerve regeneration. However, the role of Schwann cells and the pathways involved remain unclear. Thus, the aim of this study is to identify the effects of melatonin on Schwann cell proliferation, dedifferentiation, and the involvement of nuclear factor kappa light chain enhancer of activated B cells (NF-ĸB), focal adhesion kinase (FAK) and proto-oncogene tyrosine-protein kinase, Src pathways in this process. Methods: Schwann cells was treated with melatonin and its proliferation and dedifferentiation were identified using MTT assay and immunofluorescence staining for SRY (sex determining region Y)-box 2 (SOX2). Next, the protein expressions of NF-ĸB, FAK and Src pathways were identified by Western blot. Results: MTT results confirmed increased proliferation of Schwann cells with melatonin treatment, and it was highest at 10 μM melatonin. Immunofluorescent staining revealed an increase in the green fluorescence staining for SOX2 in melatonin-treated cells, showing enhanced dedifferentiation. Western blot assay revealed melatonin increased phospho-NF-ĸB (PNF-ĸB), IKK-α, FAK (D2R2E), phospho-FAK (Tyr 576/577 and Tyr 397) protein expressions as compared with control. However, Src (32G6), Lyn (C13F9), Fyn, Csk (C74C1) protein expressions were not increased as compared with control. Conclusions: Melatonin promotes Schwann cell proliferation and dedifferentiation via NF-ĸB, FAKdependent but Src-independent pathways.

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Recent Advances in Computational Modeling of Multi-targeting Inhibitors as Anti-Alzheimer Agents

Thai,

Tran,

Tran

et al. 2023

Neuromethods

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Effects of Amyloid Precursor Protein Overexpression on NF-κB, Rho-GTPase and Pro-Apoptosis Bcl-2 Pathways in Neuronal Cells

Cited by 5 publications

References 44 publications

Effects of Aerobic Interval Training Along with Ginkgo biloba Supplementation on Passive Avoidance Memory and GDNF Gene Expression in Alzheimer Rats

Effects of Aerobic Interval Training Along with Ginkgo biloba Supplementation on Passive Avoidance Memory and GDNF Gene Expression in Alzheimer Rats

Melatonin Induced Schwann Cell Proliferation and Dedifferentiation Through NF-ĸB, FAKDependent but Src-Independent Pathways

Recent Advances in Computational Modeling of Multi-targeting Inhibitors as Anti-Alzheimer Agents

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