Effects of an ectomycorrhizal fungus on the growth and physiology of Pinus sylvestris var. mongolica seedlings subjected to saline–alkali stress

Yin, Dafei; Halifu, Saiyaremu; Song, Ruiqing; Jing, Qi; Deng, Xun; Deng, Junliang

doi:10.1007/s11676-019-01007-7

Cited by 20 publications

(10 citation statements)

References 31 publications

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“…The exclusion mechanism mainly minimizes the occurrence of harmful interaction in the apoplast by hindering the entry of Al into the cytosol [ 56 ]. The symbiotic role of ectomycorrhizal fungi in Pinus species is also well known to naturally cope with the different biotic and abiotic stresses and increase immune response towards Al toxicity [ 5 , 44 , 57 , 58 , 59 , 60 ]. S. luteus , an ectomycorrhizal fungus, is well-known for its growth habit in adverse soil conditions, i.e., saline soils, drought, and metal toxicity [ 27 , 44 , 61 , 62 , 63 , 64 , 65 ].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Insight into the Symbiotic Relationship of Pinus massoniana Lamb and Suillus luteus towards Developing Al-Stress Resistance

Liu

Chen²,

Ding³

et al. 2021

Life

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Global warming significantly impacts forest range areas by increasing soil acidification or aluminum toxicity. Aluminum (Al) toxicity retards plant growth by inhibiting the root development process, hindering water uptake, and limiting the bioavailability of other essential micronutrients. Pinus massoniana (masson pine), globally recognized as a reforestation plant, is resistant to stress conditions including biotic and abiotic stresses. This resistance is linked to the symbiotic relationship with diverse ectomycorrhizal fungal species. In the present study, we investigated the genetic regulators as expressed proteins, conferring a symbiotic relationship between Al-stress resistance and Suillus luteus in masson pine. Multi-treatment trials resulted in the identification of 12 core Al-stress responsive proteins conserved between Al stress conditions with or without S. luteus inoculation. These proteins are involved in chaperonin CPN60-2, protein refolding and ATP-binding, Cu-Zn-superoxide dismutase precursor, oxidation-reduction process, and metal ion binding, phosphoglycerate kinase 1, glycolytic process, and metabolic process. Furthermore, 198 Al responsive proteins were identified specifically under S. luteus-inoculation and are involved in gene regulation, metabolic process, oxidation-reduction process, hydrolase activity, and peptide activity. Chlorophyll a-b binding protein, endoglucanase, putative spermidine synthase, NADH dehydrogenase, and glutathione-S-transferase were found with a significant positive expression under a combined Al and S. luteus treatment, further supported by the up-regulation of their corresponding genes. This study provides a theoretical foundation for exploiting the regulatory role of ectomycorrhizal inoculation and associated genetic changes in resistance against Al stress in masson pine.

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Section: Discussionmentioning

confidence: 99%

Proteomic Insight into the Symbiotic Relationship of Pinus massoniana Lamb and Suillus luteus towards Developing Al-Stress Resistance

Liu

Chen²,

Ding³

et al. 2021

Life

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“…This symbiosis is a ubiquitous mutualism that plays a key role in plant and soil health and in carbon and nutrient cycles [7,8]. Several studies have demonstrated that inoculating with ECM fungi is an effective method to improve seedling resistance to biotic and abiotic stresses, to promote growth, shorten the survival stage, and improve the survival rate in afforestation [9][10][11][12][13][14][15][16][17][18]. In addition, since some ECM fungi have a high economic value as an edible or medicinal product, the use of ECM as a practice in agroforestry has the potential to increase grower income [17,19].…”

Section: Introductionmentioning

confidence: 99%

“…However, other experiments resulted in smaller plants [16,[20][21][22]. The role and effect of ECM on plant growth differed depending on the associated fungal species [17,22,23], the nutritional status of the plant [16,24], and the environment [11,22]. Previous studies have primarily focused on the effects of B. edulis on C. mollissima and Pinus species with inconsistent results [13,22,25].…”

Section: Introductionmentioning

confidence: 99%

“…Seasonal variations also result in the accumulation of reactive oxygen species (ROS), which cause lipid peroxidation, enzyme inhibition, and programmed cell death [30][31][32]. Many studies suggest that ECM fungi can effectively improve plant resistance to abiotic stresses [9,11,18], which may be mediated by combinations of improved nutrient and water relations, as well as the modulation of reactive oxygen species' metabolism.…”

Section: Introductionmentioning

confidence: 99%

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Effect of an Ectomycorrhizal Fungus on the Growth of Castanea henryi Seedlings and the Seasonal Variation of Root Tips’ Structure and Physiology

Xiong

Chen

et al. 2021

Forests

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Castanea henryi is a ubiquitous hardwood chestnut species in southern China and is important both ecologically and economically. It is mainly cultivated for nut production, just like other chestnut species. However, the establishment of C. henryi seedlings in a new orchard has proven to be difficult because few seedlings survive transplanting due to the incompatibility of their coarse root architecture with nutrient-depleted red acid soils in southern China. Root architecture can be profoundly modified and nutrient can be stress alleviated due to the association of roots with ectomycorrhizal (ECM) fungi. Boletus edulis is an ECM fungus with edible and medicinal fruiting bodies. However, its impact on plant growth varies with the plant species it is associated with. In order to elucidate the role of B. edulis in C. henryi afforestation, we evaluated growth parameters and soil enzymatic activities, as well as seasonal variations in physiology and structure of ECM root tips. Growth responses and soil enzymatic activities were measured 6 months after inoculation. The physiological characteristics of root tips were also compared at various seasons throughout the year. B. edulis colonization of C. henryi roots was successful at a 60% colonization rate. Height, base diameter, and biomass (especially the underground part) of inoculated seedlings (JG) were higher than those of uninoculated seedlings (CK). JG had higher root total length, root surface area, root volume, root average diameter, and number of root tips than CK. Additionally, JG exhibited higher total nitrogen and phosphorus content. Abnormal mantle and Harting net were observed in winter. No matter the season, ECM tips had higher antioxidant enzyme activities, root activities, soluble protein content, and lower malondialdehyde compared to non-ECM tips (nE) and those without ECM tips (woE), and there were no differences between nE and woE. It is important to understand the growth of the host plant in response to ECM and that the seasonal variation of ECM root tips is important when growing high-quality C. henryi seedlings, due to the crucial role of B. edulis in improving seedling initial survival rate.

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“…In Pinus, previous studies have shown that ectomycorrhizal fungi improve plant growth and tolerance to drought stress, salinity stress, low-phosphorous stress, heavy metals toxicity, etc. [4,6,[25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Identification of candidate genes conferring tolerance to aluminum stress in Pinus massoniana inoculated with ectomycorrhizal fungus

et al. 2020

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Background Pinus massoniana Lamb. is an important afforestation tree species with high economic, ecological and medicinal values. Aluminum (Al) toxicity driven by soil acidification causes dieback of P. massoniana plantations. Previous studies showed that ectomycorrhizal fungi alleviate Al stress damages in Pinus, but the underlying molecular mechanisms and key genes induced by ectomycorrhizal fungi inoculation under Al stress in Pinus have not been explored. Herein, we applied Al stress for 60 days to P. massoniana seedlings inoculated with Suillus luteus (SL) and those non-inoculated. Then, we compared their growth parameters and transcriptome in order to detect candidate genes induced by SL conferring Al tolerance in P. massoniana. Result Our results showed that SL inoculation confers Al stress tolerance in P. massoniana through improved growth performance, strong antioxidant enzyme activities and reduced malondialdehyde accumulation as compared to non-inoculated seedlings. Transcriptome sequencing further supported these findings as very few genes (51 genes) were transcriptionally altered by Al in SL inoculated plants as compared to non-inoculated plants (2140 genes). We identified three core genes (cox1, cox3 and Nd1) that were strongly up-regulated by Al in the SL inoculated plants but were down-regulated in the non-inoculated plants. We also identified 42 genes specifically regulated by SL inoculated plants under Al stress, which are involved in a wide range of biological processes such as antioxidative response, transporters, hormone signaling and plant pathogen infection responses. Conclusions Altogether, our data suggest that SL inoculation induces priming of key stress response pathways and triggers specific genes that efficiently alleviate Al stress effects in P. massoniana. The candidate genes resources generated in this study are of utmost importance for functional characterization and molecular studies aiming at improving Al tolerance in plants.

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Effects of an ectomycorrhizal fungus on the growth and physiology of Pinus sylvestris var. mongolica seedlings subjected to saline–alkali stress

Cited by 20 publications

References 31 publications

Proteomic Insight into the Symbiotic Relationship of Pinus massoniana Lamb and Suillus luteus towards Developing Al-Stress Resistance

Proteomic Insight into the Symbiotic Relationship of Pinus massoniana Lamb and Suillus luteus towards Developing Al-Stress Resistance

Effect of an Ectomycorrhizal Fungus on the Growth of Castanea henryi Seedlings and the Seasonal Variation of Root Tips’ Structure and Physiology

Identification of candidate genes conferring tolerance to aluminum stress in Pinus massoniana inoculated with ectomycorrhizal fungus

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