Effects of anabolic implants and ractopamine-HCl on muscle fiber morphometrics, collagen solubility, and tenderness of beef longissimus lumborum steaks1,2

Ebarb, S. M.; Phelps, K. J.; Drouillard, James S.; Maddock-Carlin, K. R.; Vaughn, M. A.; Burnett, D. D.; Noel, J. A.; Krueger, C. L. Van Bibber; Paulk, Chad B.; Grieger, David M.; Gonzalez, J. M.

doi:10.2527/jas.2016.1263

Cited by 8 publications

(4 citation statements)

References 59 publications

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“…Furthermore, the net effect of increased muscle tissue ( Smith et al, 1989 ; Grant et al, 1993 ; Schroeder et al, 2003 ) is thought to be because of reduced muscle degradation ( Eadara et al, 1989 ; Wheeler and Koohmaraie, 1992 ; Scramlin et al, 2010 ) and increased protein synthesis ( Scramlin et al, 2010 ). The effects of RAC on carcass measurements were similar in this study to others in terms of reduced marbling ( Gruber et al, 2007 ; Winterholler et al, 2007 ), increased LMA ( Bryant et al, 2010 ; Boler et al, 2012 ; Ebarb et al, 2017 ), and no effect on dressing percentage ( Laudert et al, 2004 ; Quinn et al, 2008 ). Results from this study also support the findings of Thompson et al (2016) , where monensin was supplemented at 36.4 mg/kg DM and RAC at 300 mg/d for 32 d. In both studies, RAC increased LMA and G:F. RAC supplementation did not affect DMI, HCW, dressing percentage, or 12th rib fat.…”

Section: Discussionsupporting

confidence: 87%

Effects of ractopamine hydrochloride supplementation on feeding behavior, growth performance, and carcass characteristics of finishing steers1,2

Trotta

Carlin

Swanson

2019

Translational Animal Science

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Ractopamine hydrochloride (RAC) is a β-adrenergic agonist that functions as a repartitioning agent to improve muscling in feedlot cattle. Many studies have investigated the effects of RAC on growth performance and carcass characteristics; however, there is minimal information about the influence of RAC on feeding behavior. Sixty-nine steers (body weight [BW] = 364 ± 3.9 kg) predominately of Angus and Simmental breeding were subjected to a 126-d (n = 46) or 154-d (n = 23) feeding period and randomly assigned to one of two treatment groups: supplementation to provide 0 (CON; n = 34) or 267 ± 4.9 mg/d of RAC (n = 35). Ractopamine was provided as Optaflexx 45 at 0.024% of the diet (dry matter [DM] basis; Elanco Animal Health, Greenfield, IN). Dietary treatments were fed the final 42 d in the feed yard (treatment period). Feeding behavior and growth performance were measured using radio frequency identification tags and the Insentec feeding system. Following the final day of treatment, steers were slaughtered and carcass measurements were recorded. Data were analyzed using MIXED models in SAS. There were no differences in BW, average daily gain (ADG), DM intake (DMI), gain:feed ratio (G:F), or feeding behavior during the pretreatment period (P > 0.44). Ractopamine supplementation increased G:F during the treatment period (P = 0.02) and during the total period (P = 0.03) and tended to increase ADG during the treatment and total period (P ≤ 0.08). DMI was not affected during the treatment or total period (P > 0.67). Eating time per visit, per meal, and per day were decreased (P < 0.02) in steers supplemented with RAC during the treatment period. DMI per minute was increased (P = 0.02) in steers supplemented with RAC. Hot carcass weight, dressing percentage, and 12th rib fat were not influenced by RAC supplementation. Ractopamine supplementation decreased marbling (P = 0.008) and kidney, pelvic, and heart percentage (P = 0.04) and increased longissimus muscle area (P = 0.01). These data demonstrate that RAC supplementation for 42 d improves feed efficiency, increases the rate of DMI without altering DMI, and increases muscling in finishing cattle.

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Section: Discussionsupporting

confidence: 87%

Effects of ractopamine hydrochloride supplementation on feeding behavior, growth performance, and carcass characteristics of finishing steers1,2

Trotta

Carlin

Swanson

2019

Translational Animal Science

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“…Similar to the current study, there were no effect of RAC on collagen content and collagen solubility in heifer [29] and in steer [30]. In this study, gilts had significant higher content of insoluble collagen and tended to have higher content of total collagen and percentage of collagen solubility than barrows.…”

Section: Chemical Composition and Collagen Solubilitysupporting

confidence: 75%

Effect of ractopamine on physicochemical characteristics and collagen solubility in finishing pigs

et al. 2018

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Abstract. The objective of this study was to investigate physicochemical characteristics and collagen solubility of pigs fed with dietary ractopamine (RAC) during finishing period. Thirty of three-way crossbred pigs (Large White × Landrace × Duroc) with an initial bodyweight (BW) of 18.0 ± 0.5 kg were randomly assigned to one to three dietary RAC inclusions (0, 20, or 40 mg/kg) fed during finishing period (60-100 kg BW). Each group consisted of five gilts and five barrows. After 109 day of raising period, all pigs were slaughtered at the average weight of 100 kg. Longissimus dorsi muscle (LD) was taken from each carcass after overnight chill and analyzed for physical characteristics, chemical composition, and collagen solubility. The LD from pigs fed 40 mg/kg of RAC had lower a* value (p < 0.01) than others. Pigs fed with 40 mg/kg of RAC had higher Warner-Bratzler shear force (p < 0.05) than pigs fed without RAC. Pigs fed with dietary RAC had lower glycogen content than control (p < 0.01). While other chemical compositions and collagen solubility of LD were not affected by dietary RAC (p > 0.05). Gilts had larger muscle fibers (p < 0.01), lower glycogen content (p < 0.05), higher content of insoluble collagen (p < 0.05), total collagen (p = 0.054), and collagen solubility (p = 0.054) than barrows.

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“…The four MyHC isoforms, namely, I, IIa, IIx, and IIb, are classified by different coding genes, which leads to four myofiber types. MyHC I is expressed in type I fibers, and IIa, IIx, and IIb are expressed in IIA, IIX, and IIB fiber types, respectively [ 53 , 54 ]. The two subunits of the MyHC I heavy chain are composed of β-cardiac and α-cardiac encoded by MYH7 and MYH6, respectively [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Expression profile and bioinformatics analysis of circRNA and its associated ceRNA networks in longissimus dorsi from Lufeng cattle and Leiqiong cattle

Yang,

Wu,

Guo

et al. 2023

BMC Genomics

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This paper aims to explore the role of circRNA expression profiles and circRNA-associated ceRNA networks in the regulation of myogenesis in the longissimus dorsi of cattle breeds surviving under subtropical conditions in southern China by RNA sequencing and bioinformatics analysis. It also aims to provide comprehensive understanding of the differences in muscle fibers in subtropical cattle breeds and to expand the knowledge of the molecular networks that regulate myogenesis. With regard to meat quality indicators, results showed that the longissimus dorsi of LQC had lower pH (P < 0.0001), lower redness (P < 0.01), lower shear force (P < 0.05), and higher brightness (P < 0.05) than the longissimus dorsi of LFC. With regard to muscle fiber characteristics, the longissimus dorsi of LQC had a smaller diameter (P < 0.0001) and higher density of muscle fibers (P < 0.05). The analysis results show that the function of many circRNA-targeted mRNAs was related to myogenesis and metabolic regulation. Furthermore, in the analysis of the function of circRNA source genes, we hypothesized that btacirc_00497 and btacirc_034497 may regulate the function and type of myofibrils by affecting the expression of MYH6, MYH7, and NEB through competitive linear splicing.

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Effects of anabolic implants and ractopamine-HCl on muscle fiber morphometrics, collagen solubility, and tenderness of beef longissimus lumborum steaks1,2

Cited by 8 publications

References 59 publications

Effects of ractopamine hydrochloride supplementation on feeding behavior, growth performance, and carcass characteristics of finishing steers1,2

Effects of ractopamine hydrochloride supplementation on feeding behavior, growth performance, and carcass characteristics of finishing steers1,2

Effect of ractopamine on physicochemical characteristics and collagen solubility in finishing pigs

Expression profile and bioinformatics analysis of circRNA and its associated ceRNA networks in longissimus dorsi from Lufeng cattle and Leiqiong cattle

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