Effects of analogues of substance P fragments on the MAO activity in rat brain

Turska, Ewa; Lachowicz, Lilla; Koziołkiewicz, Wiktor; Wasiak, T.

doi:10.1016/0306-3623(85)90088-6

Cited by 4 publications

(5 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The activity of MAO measured for NKB, Ab (25-35) and the combined peptide treatment of , showed an increased enzyme activity and with a further increase in the concentration of the peptide decreased the enzyme activity when compared with that of the age matched untreated controls in 3 months at lower concentration. In the other age groups like 9, 18 and 30 months, with increase in concentration of NKB, a decrease in the enzyme activity was observed, indicating enzyme inhibition, the present results are in accord with that of Turska et al (1985). The combined incubation of the brain synaptosomes with increase in peptide concentrations reversed back the amyloid induced increase in MAO activity in different age groups.…”

Section: Discussionsupporting

confidence: 94%

Neuroprotective Role of Neurokinin B (NKB) on β-amyloid (25–35) Induced Toxicity in Aging Rat Brain Synaptosomes: Involvement in Oxidative Stress and Excitotoxicity

et al. 2006

View full text Add to dashboard Cite

The brain tissue has a large oxidative capacity, but its ability to combat oxidative stress is limited. In aging brain tissue the oxidative stress increases due to decreased activity of antioxidant enzymes and increased oxidative stress leading to neurodegeneration associated with excitotoxicity. The aim of the present study was to determine the effect of neuropeptides, neurokinin B (NKB) and amyloid beta protein fragment Abeta (25-35) and neurotransmitters N-methyl D-aspartate (NMDA) and Glutamate on rat brain synaptosomes of different age groups. Aging brain functions were assessed by measuring the activities of superoxide dismutase (Mn-SOD) and monoamine oxidase (MAO) and intrasynaptosomal [Ca(2+)](i )levels in presence of neuropeptides and neurotransmitters. Increase in age decreased the SOD and MAO enzyme activities; Abeta (25-35) addition further had damaging/toxic effects on the enzymes, whereas NKB alone and in combination with amyloid lowered the toxic effects caused by Abeta (25-35) addition, which was concentration (peptide) and age dependent. Oxidative stress and excitotoxicity are major consequences associated with the age, [Ca(2+)](i )was increased with the age and the neuropeptides and neurotransmitters elicited significant modulatory effects on it. Our study elucidates an increased activity of SOD, decreased activity of MAO and restoration of [Ca(2+)](i) levels in the presence of NKB and suggests an antioxidant, neuromodulatory and neuroprotective role of tachykinin peptide NKB against the beta amyloid induced toxicity.

show abstract

Section: Discussionsupporting

confidence: 94%

Neuroprotective Role of Neurokinin B (NKB) on β-amyloid (25–35) Induced Toxicity in Aging Rat Brain Synaptosomes: Involvement in Oxidative Stress and Excitotoxicity

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Age related alterations of brain Na + -K + ATPase activity has been reported earlier [39] [40]. The activity of Na + -K + ATPase was measured using different concentrations of Aβ (25 -35), NKB and combined treatment of NKB and Aβ (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) in different age groups of control (without E2 treated) and E2 treated rat brain synaptosomes. In different concentration of Aβ (25 -35) incubation, the enzyme Na + -K + ATPase inhibited in all the age groups when compared with age matched controls.…”

Section: Discussionmentioning

confidence: 89%

“…They are known to reduce oxidative stress in the brain [26]- [28], and to reverse the neurotoxic effects of Aβ in neurons and a role in neurodegenerative diseases [29] [30]. The SP or related tachykinins exert a neurotrophic action on central neurons and the loss of this input in selective areas results, in the expression of neurodegenerative disorders [31].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Potential of 17β Estradiol with Tachykinin Neuropeptide NKB and Aβ(25 - 35) on Na+ - K+ ATPase Activity in Aging Female Rat Brain

Jha¹,

Mishra²,

Kumar³

et al. 2015

AAR

View full text Add to dashboard Cite

The Na + -K + ATPase is an enzyme responsible for the active transport of Na + and K + in most eukaryotic cells. The aim of the present study was to determine the effect of Tachykinin neuropeptide, Neurokinin B (NKB) and Amyloid beta fragment Aβ (25 -35) on 17β estradiol (E2) treated aging female rat brain synaptosomes of different age groups, by assaying Na + -K + ATPase enzyme activity. An in vitro incubation of isolated synaptosomes with Aβ (25 -35) showed toxic effects while NKB showed stimulating effect on the Na + -K + ATPase activity, and the combined NKB + Aβ (25 -35) incubations showed a partial effect as compared to the Aβ (25 -35) alone. To understand whether E2 affects the expression of Na + -K + ATPase molecules, we examined the expression of Na + -K + ATPase subunit α1 and β2 in E2 treated aging female rat brain synaptosomes. The enzyme was quantified by SDS PAGE in control and E2 treated rat brain. We observed that the expression of α1 and β2 Na + -K + ATPase molecules increased and reversed to a normal level in E2 treated synaptosomes. These results confirmed that E2 increased turnover of Na + -K + ATPase molecules in aging rat brain. The present findings also suggest a possible role of NKB with E2 in the age related changes in the brain.

show abstract

“…These tachykinin neuropeptides have significant sequence similarity with Aβ (25 -35) which forms neurotoxin amyloids found to be nontoxic in neuronal cells [15]. The tachykinins are known to reduce oxidative stress in the brain [16][17][18], to reverse the neurotoxic effects of Aβ in neurons and to play a role in neurodegenerative diseases [19,20].…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective role of 17β estradiol with tachykinin neuropeptide NKB and Aβ (25 - 35) in aging female rat brain

Jha¹,

Mahdi²,

Pandey³

et al. 2013

AAR

View full text Add to dashboard Cite

The brain experiences structural, molecular and functional alterations during aging. In aging brain tissue, the oxidative stress increases due to decreased activity of antioxidant enzymes and increased oxidative stress leading to neurodegeneration associated with excitotoxicity. In the present study, we observed the effect of tachykinin neuropeptide Neurokinin B (NKB) and amyloid beta fragment Aβ (25-35) on the activity of Acetylcholine esterase (AChE) and Lipid peroxidation (LPO) in brains of 17β estradiol (E2) treated aging female rat synaptosomes of different age groups. An in-vitro incubation of E2 treated brain synaptosomes with Aβ (25-35) showed toxic effects on all the parameters. The treatment of NKB and combined NKB and Aβ (25-35) increased the AChE enzyme activity and decreased the level of LPO in E2 treated aging rats. The treatment of NKB and combined NKB and Aβ (25-35) in a concentration dependent manner reversed the effects of aging and Aβ (25-35) on AChE and LPO. The present finding suggests that E2 along with NKB reverse aging and Aβ (25-35) induced toxicity as well as AChE and LPO levels. The results of the current study showed a possible beneficial role of NKB with E2 in the age related neurological diseases.

show abstract

Effects of analogues of substance P fragments on the MAO activity in rat brain

Cited by 4 publications

References 16 publications

Neuroprotective Role of Neurokinin B (NKB) on β-amyloid (25–35) Induced Toxicity in Aging Rat Brain Synaptosomes: Involvement in Oxidative Stress and Excitotoxicity

Neuroprotective Role of Neurokinin B (NKB) on β-amyloid (25–35) Induced Toxicity in Aging Rat Brain Synaptosomes: Involvement in Oxidative Stress and Excitotoxicity

Therapeutic Potential of 17<i>β</i> Estradiol with Tachykinin Neuropeptide NKB and A<i>β</i>(25 - 35) on Na<sup>+</sup> - K<sup>+</sup> ATPase Activity in Aging Female Rat Brain

Neuroprotective role of 17<i>β</i> estradiol with tachykinin neuropeptide NKB and A<i>β</i> (25 - 35) in aging female rat brain

Contact Info

Product

Resources

About

Effects of analogues of substance P fragments on the MAO activity in rat brain

Cited by 4 publications

References 16 publications

Neuroprotective Role of Neurokinin B (NKB) on β-amyloid (25–35) Induced Toxicity in Aging Rat Brain Synaptosomes: Involvement in Oxidative Stress and Excitotoxicity

Neuroprotective Role of Neurokinin B (NKB) on β-amyloid (25–35) Induced Toxicity in Aging Rat Brain Synaptosomes: Involvement in Oxidative Stress and Excitotoxicity

Therapeutic Potential of 17&lt;i&gt;β&lt;/i&gt; Estradiol with Tachykinin Neuropeptide NKB and A&lt;i&gt;β&lt;/i&gt;(25 - 35) on Na&lt;sup&gt;+&lt;/sup&gt; - K&lt;sup&gt;+&lt;/sup&gt; ATPase Activity in Aging Female Rat Brain

Neuroprotective role of 17&lt;i&gt;β&lt;/i&gt; estradiol with tachykinin neuropeptide NKB and A&lt;i&gt;β&lt;/i&gt; (25 - 35) in aging female rat brain

Contact Info

Product

Resources

About

Therapeutic Potential of 17<i>β</i> Estradiol with Tachykinin Neuropeptide NKB and A<i>β</i>(25 - 35) on Na<sup>+</sup> - K<sup>+</sup> ATPase Activity in Aging Female Rat Brain

Neuroprotective role of 17<i>β</i> estradiol with tachykinin neuropeptide NKB and A<i>β</i> (25 - 35) in aging female rat brain