Effects of anesthetic alcohols on membrane transport processes in human erythrocytes

Kutchai, Howard; Chandler, Laura H.; Geddis, Lisa M.

doi:10.1016/0005-2736(80)90490-3

Cited by 22 publications

(3 citation statements)

References 41 publications

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“…In a likewise manner, ethanol was also unable to significantly inhibit Glc transport (Table 1) at pharmacologically relevant concentrations (i.e., Յ10 mM). Inhibition of GLUT-1 mediated Glc transport by ethanol has, however, been observed at suprapharmacological concentrations (Kutchai, Chandler & Geddis, 1980;Krauss, Diamond & Gordon, 1994, and not shown). Additionally, the halogenated hydrocarbons and ethanol had no significant effect on GLUT-1-mediated Glc transport, i.e., unidirectional Glc flux, in human erythrocytes (Honkanen et al, 1995;El-Barbary et al, 1996, and not shown).…”

Section: Glut-1 Mediated Glc Transport In 3t3-c2 Cells Is Inhibited Bmentioning

confidence: 99%

Effects of Barbiturates on Facilitative Glucose Transporters are Pharmacologically Specific and Isoform Selective

et al. 1999

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Barbiturates inhibit GLUT-1-mediated glucose transport across the blood-brain barrier, in cultured mammalian cells, and in human erythrocytes. Barbiturates also interact directly with GLUT-1. The hypotheses that this inhibition of glucose transport is (i) selective, preferring barbiturates over halogenated hydrocarbon inhalation anesthetics, and (ii) specific, favoring some GLUT-# isoforms over others were tested. Several oxy- and thio-barbiturates inhibited [3H]-2-deoxyglucose uptake by GLUT-1 expressing murine fibroblasts with IC50s of 0.2-2.9 mm. Inhibition of GLUT-1 by barbiturates correlates with their overall lipid solubility and pharmacology, and requires hydrophobic side chains on the core barbiturate structure. In contrast, several halogenated hydrocarbons and ethanol (all 10 mm). Thus, barbiturates selectively inhibit glucose transport by some, but not all, facilitative glucose transporter isoforms.

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Section: Glut-1 Mediated Glc Transport In 3t3-c2 Cells Is Inhibited Bmentioning

confidence: 99%

Effects of Barbiturates on Facilitative Glucose Transporters are Pharmacologically Specific and Isoform Selective

et al. 1999

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“…[2][3][4] Drug treatment including treatment with estrogens, gentamicin, anesthetic alcohols (pentanol, hexanol and heptanol), and salicylic acid can also alter membrane fluidity. 2,5,6 Cholesterol is an essential constituent of the membranes of mammalian cells and is involved in normal cell growth and function. It is mainly located in the cell plasma membrane and acts as the main lipid rigidifier in natural membranes under physiological conditions.…”

Section: Introductionmentioning

confidence: 99%

Modulation of sulfate renal transport by alterations in cell membrane fluidity

Lee

Balasubramanian

Murer

et al. 1999

Journal of Pharmaceutical Sciences

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Changes in membrane fluidity have been shown to alter the sodium-dependent renal transport of glucose and phosphate; however, this has not been examined for sodium/sulfate cotransport in the renal proximal tubule. Sodium/sulfate cotransport regulates the homeostasis of sulfate in mammals. The objective of this study was to investigate the influence of alterations of membrane fluidity on sodium-coupled sulfate transport in the Madin-Darby canine kidney cells, which have been stably transfected with sodium/sulfate cotransporter (NaSi-1) cDNA (MDCK-Si). Preincubation of cells with 0. 2 mM cholesterol significantly decreased the V(max) for sodium/sulfate cotransport (13.69 +/- 1.11 vs 10.15 +/- 1.17 nmol/mg protein/5 min, mean +/- SD, n = 4, p < 0.01) with no significant alteration in K(m). The addition of benzyl alcohol (20 mM) to cells increased the V(max) of sulfate uptake by 20% (11.97 +/- 0.91 vs 14. 35 +/- 0.56 nmol/mg protein/5 min, mean +/- SD, n = 3, p < 0.05) with no significant change in K(m). Membrane fluidity, as measured by the fluorescence polarization of 1,6-diphenyl 1,3,5-hexatriene (DPH), was significantly increased in MDCK-Si cells treated with 20 mM benzyl alcohol and decreased in the cells preincubated with 0.2 mM cholesterol, compared with control cells. Our results suggest that alterations in membrane fluidity that may occur as a result of disease states, aging, and pregnancy may play an important role in the modulation of renal sodium/sulfate cotransport.

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“…ithelial membranes, it has been established that changes in the physical state of the lipids influence membrane activities, both protein and non-protein mediated, particularly transmembrane transport activities (Houslay et al, 1980;Kutchai et al, 1980;Livingstone & Schachter, 1980;Yuli et al, 1981; Brasitus & Dudeja, 1985; Ives & Verkman, 1985; Worman et al, 1986;Verkman & Ives, 1986). The link between membrane enzymic or transport activities and the lipid state has not been resolved, due in part to the difficulties in defining and measuring accurately lipid states in natural membranes which contain large quantities of heterogeneous proteins and cholesterol.…”

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confidence: 99%

Lipid domain structure correlated with membrane protein function in placental microvillus vesicles

Illsley¹,

Lin²

1987

Biochemistry

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Membrane fluidity properties of placental microvillus membrane vesicles (MVV) were determined from fluorescence anisotropy (r), dynamic depolarization, and lifetime heterogeneity studies of diphenylhexatriene (DPH), trimethylamino-DPH (TMA-DPH), and cis- and trans-parinaric acids (c-PnA and t-PnA). Plots of r against temperature for DPH and TMA-DPH in MVV had slope discontinuities at 26 degrees C (Tc, transition temperature); however, analysis of r in terms of probe rotational rate (R), limiting anisotropy (r infinity), and lifetime (tau) revealed that DPH reported a phase transition because of changes in r infinity, whereas the phase transition observed by TMA-DPH occurred primarily because of changes in R. Heterogeneity analysis using phase and modulation lifetimes at three frequencies showed that DPH and TMA-DPH lifetimes were homogeneous in MVV. Both long (greater than 25 ns) and short (less than 6 ns) lifetime components were detected for c-PnA and t-PnA in MVV, corresponding to the probes in solid and fluid lipid phases. The fractional amplitude of the long lifetimes (solid phase) decreased from 0.86 to 0.12 with increasing temperature (5-55 degrees C) as the membrane passed through the phase transition, with 50% of the change occurring at 27 degrees C (c-PnA) or 33 degrees C (t-PnA). The activation energies for alkaline phosphatase, aminopeptidase M, and sodium-proton antiporter activities all showed discontinuities in the temperature range 27-31 degrees C.(ABSTRACT TRUNCATED AT 250 WORDS)

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Effects of anesthetic alcohols on membrane transport processes in human erythrocytes

Cited by 22 publications

References 41 publications

Effects of Barbiturates on Facilitative Glucose Transporters are Pharmacologically Specific and Isoform Selective

Effects of Barbiturates on Facilitative Glucose Transporters are Pharmacologically Specific and Isoform Selective

Modulation of sulfate renal transport by alterations in cell membrane fluidity

Lipid domain structure correlated with membrane protein function in placental microvillus vesicles

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