Effects of angiotensin, catecholamines and cyclic AMP on calcium storage in aortic microsomes

Baudouin-Legros, Maryvonne; Meyer, Philippe

doi:10.1111/j.1476-5381.1973.tb08335.x

Cited by 95 publications

(34 citation statements)

References 23 publications

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“…Andersson and Nilsson (11), using the microsomal fraction from rabbit colon, found that the Ca-accumulating capacity of the microsomal fractions was potentiated by cyclic AMP. Similar findings were also reported in the microsomal fractions from rabbit aorta (12) and from rat uterus (13). Therefore, potentiation of the Ca-binding in the microsomal fractions, as induced by papaverine may be due to elevation in the levels of cyclic AMP with the inhibition of phosphodiesterase.…”

Section: Methodssupporting

confidence: 77%

Pharmacological Studies of Lycorenine, an Alkaloid of Lycoris Radiata Herb.: II. Effects of Blood Pressure in Rats and Dogs and the Mechanism of Tachyphylaxis to the Vasodepressor Action of Lycorenine in Rats

Takayanagi

Hisayama

Suzuki

1980

Japanese Journal of Pharmacology

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Abstract-Effects of nonspecific smooth muscle relaxants, papaverine (10-' M) and Aspaminol (I,1-diphenyl-3-piperidinobutanol hydrochloride, 10-' M), and Ca-blocker, D-600 (10-6 M) on Ca-release and Ca-binding in the microsomal fractions of rabbit taenia coli were determined.The doses necessary to relax the K-depolarized taenia coli maximally were used. Ca-binding in the microsomal fractions was inhibited by Aspaminol and D-600 and increased by papaverine in the concentration used. On the other hand, Ca-release from the Ca-incorporated microsomal fractions was inhibited by papaverine and Aspaminol but was not influenced by D-600. Changes observed in Ca-transfer may be related to relaxation of smooth muscle induced by the test drugs.It was reported that nonspecific smooth muscle relaxants, papaverine and Aspaminol, and Ca-blockers inhibited the responses of K-depolarized intestinal smooth muscle to CaCl2 and also decreased Ca-uptake by the depolarized intestinal smooth muscle from the bath fluid (1). Since papaverine is a potent inhibitor of cyclic AMP-phosphodiesterase (2), relaxation of smooth muscle is considered to be partly due to an increase of intracellular levels of cyclic AMP. However, Aspaminol and Ca-blockers are found to have little in hibitory action on phosphodiesterase activity (3, 4). Nonspecific inhibitory action of Aspaminol (5, 6) and of Ca-blockers (7) can be decreased to a great extent by increasing the Ca-concentration in the bath fluid, while that of papaverine is only slightly increased. As little is known of the differences in the effects of the smooth muscle relaxants and Ca-blockers on Ca-release and Ca-binding in the microsomal fractions from the intestinal smooth muscle and of differences in changes in Ca-transfer induced by the nonspecific smooth muscle relaxants and Ca-blockers, we carried out studies in an attempt at elucidation. MATERIALS AND METHODSA piece (3 to 4 cm) of the taenia coli isolated from male rabbits, weighing 2 to 3 kg, was suspended in a 20 ml organ bath filled with a KCl-solution kept at 32'C and gassed with a mixture of 95% 02 and 5 % C02. Responses to drugs were recorded isotonically.The KCI-solution used had the following composition (mM) : KCl 159.6, CaCl2 2.2, MgCl2 2.1, NaHCO3 5.9 and glucose 2.8.Estimation of Ca-binding in the microsomal fractions was carried out by the method of Carsten (8)

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Section: Methodssupporting

confidence: 77%

Pharmacological Studies of Lycorenine, an Alkaloid of Lycoris Radiata Herb.: II. Effects of Blood Pressure in Rats and Dogs and the Mechanism of Tachyphylaxis to the Vasodepressor Action of Lycorenine in Rats

Takayanagi

Hisayama

Suzuki

1980

Japanese Journal of Pharmacology

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“…Tomiyama and Hurwitz (26) reported that papaverine and aminophylline appear to exert their principal actions on cellular processes that remove Ca ions from the cytoplasm. It was reported that papaverine and dibutyryl cyclic AMP augment Ca efflux from smooth muscle cells and Ca uptake by microsomal fraction (15,17,27). Consequently it is conceivable that the nonspecific inhibition by papaverine and theophylline is at least in part mediated by cyclic AMP.…”

Section: Discussionmentioning

confidence: 99%

Smooth muscle relaxing drugs and guinea pig ileum.

et al. 1981

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Abstract-The effects of various smooth muscle relaxing drugs on con tractile responses to acetylcholine (ACh), Ba2+ and Ca", and on the tissue cyclic AMP levels were examined in the guinea pig ileum. Papaverine and theophylline caused a decrease both in the maximum height and the slope of dose-response curves induced by the three stimulants, and an increase in the cyclic AMP levels.Diltiazem and D-600 produced a decrease in the maximum and the slope of ACh and Ba2+ dose-response curves, shifted the Ca" dose-response curves to higher concentrations, in a parallel manner, but failed to change the cyclic AMP levels. Etomidoline and benactyzine shifted the curves for the three stimulants in parallel to the right, but at higher concentrations depressed the maximum of ACh and Ba2+ responses with a further parallel shift. These drugs exerted little influence on the basal level of tissue cyclic AMP, but etomidoline signifi cantly depressed the Bat+-induced increase in cyclic AMP level. The smooth muscle relaxing drugs used could be classified in three types, thereby suggesting that there are at least three different mechanisms involved in smooth muscle relaxing action.

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“…Other workers reported that epinephrine, iso prenaline and papaverine, which increase intracellular levels of cyclic AMP, increased 45Ca-uptake by the microsomal fractions from some smooth muscles (4,14,26,27). On the other hand, some smooth muscle relaxants, which have no influence on the intracellular levels of cyclic AMP, inhibited 45Ca-uptake by the intestinal microsomal fractions (13, 27 29).…”

Section: Discussionmentioning

confidence: 99%

Inhibitory alpha-adrenergic action of phenylephrine in guinea pig taenia caecum

Iwayama

Takayanagi

Kasuya

1979

Japanese Journal of Pharmacology

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Abstract-Phenylephrine, a selective a-adrenergic stimulant, caused a maximal re laxation of the taenia from guinea pig caecum in the concentration of 10-6 g/ml. Phenylephrine in this concentration did not influence intracellular cyclic AMP and cyclic GMP levels. Although phenylephrine abolished the spontaneous spike discharge, no change was detected in "Ca-uptake and "Ca-efflux on the tissue level after phenyl ephrine. Ca-uptake and Ca-release on the subcellular level were also not influenced by phenylephrine.In Ca free-solution phenylephrine inhibited the response to CaCl2. Phenylephrine increased 42K-efflux in the normally polarized taenia and also in the K-depolarized taenia.Adrenoceptors were classified as a and 3-receptors by Ahlquist (1). As a rule, a receptor is associated with the excitatory functions and 3-receptor with the inhibitory functions. Exceptionally, stimulation of a and (9-receptors in an intestinal smooth muscle causes a relaxation (2). But the inhibitory responses mediated through a and (3-receptors are independent as to mechanical effects and metabolic effects (3).It was reported that stimulation of Q-adrenoceptors elevates the intracellular levels of cyclic AMP and facilitates Ca-accumulation (4). However, little is known of the mechanisms involved in the responses mediated through a-adrenoceptors, except that an a-adrenoceptor stimulant increases K-permeability (5-7). We carried out studies on the effects of an a adrenoceptor stimulant on tissue levels of cyclic nucleotides, Ca-movement and K permeability. MATERIALS AND METHODS Phenylephrinewas used as the a-adrenergic stimulant.Male guinea pigs (300-400 g)were sacrificed by a blow on the neck and two pieces of taenia were removed from one caecum and suspended in two 30 ml organ baths filled with physiological solution kept at 32'C and bubbled with air. One was used for measuring the control levels of cyclic nucleotides and the other for estimating the change in levels of cyclic nucleotides after one min treatment with phenylephrine in the presense of propranolol. They were frozen in liquid nitrogen.Cyclic AMP was assayed with the method of Gilman (8)

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Effects of angiotensin, catecholamines and cyclic AMP on calcium storage in aortic microsomes

Cited by 95 publications

References 23 publications

Pharmacological Studies of Lycorenine, an Alkaloid of Lycoris Radiata Herb.: II. Effects of Blood Pressure in Rats and Dogs and the Mechanism of Tachyphylaxis to the Vasodepressor Action of Lycorenine in Rats

Pharmacological Studies of Lycorenine, an Alkaloid of Lycoris Radiata Herb.: II. Effects of Blood Pressure in Rats and Dogs and the Mechanism of Tachyphylaxis to the Vasodepressor Action of Lycorenine in Rats

Smooth muscle relaxing drugs and guinea pig ileum.

Inhibitory alpha-adrenergic action of phenylephrine in guinea pig taenia caecum

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