Effects of animal liver and bile extracts on biochemical values of rat ethanol‐induced fatty liver

Wan, Tien-Chun; Liu, Yu-Tse; Duann, Lan-Tyi; Yu, Kuo-Hui; Chen, Chih‐Ming; Lin, Liang‐Chuan; Sakata, Ryoichi

doi:10.1111/asj.12088

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…The majority of ALB is synthesized in the liver. Therefore, variation of serum TP or ALB concentrations can reflect liver health status (Wan et al, 2014). In the current study, a marked decrease of serum TP and ALB levels were observed in PCM-hepatotoxic rats compared with the normal control animals.…”

Section: Serum and Liver Biochemical Markerssupporting

confidence: 53%

Hepatoprotective Potential of Astragalus kurdicus and Astragalus cinereus Extracts against Paracetamol Induced Liver Damage in Rats

Yusufoğlu¹,

Soliman²,

Abdel-Rahm³

et al. 2015

Pakistan J. of Biological Sciences

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The objective of this study was to investigate the potential hepatoprotective effect of the ethanol extracts of Astragalus kurdicus Boiss. var. kurdicus (A. kurdicus) and Astragalus cinereus Willd. (A. cinereus) in a rat model of paracetamol (PCM) induced liver damage. Paracetamol administration caused severe hepatic damage in rats as evidenced by elevated serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), γ-glutamyl transferase (γ-GT) and serum level of total bilirubin (BRN) while decreased serum levels of total protein (TP) and albumin (ALB). In liver homogenates, PCM elevated malondialdehyde (MDA) but decreased glutathione (GSH) levels as well as glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT) activities. Administration of A. kurdicus and A. cinereus extracts (200 and 400 mg kgG 1) for 7 days before PCM inhibited the elevation of the serum activities of ALT, AST, ALP and γ-GT enzymes and serum level of BRN. Moreover, they elevated the serum level of TP. Paracetamol-induced lipid peroxidation was also reduced by both extracts. Likewise, both extracts increased the activities of the antioxidant enzymes (GPx, SOD, CAT) in the liver homogenates and reduced GSH concentration. The results of the in vitro antioxidant effect revealed marked antioxidant activity for both extracts. The histopathological analysis suggested that both extracts obviously alleviated the degree of liver damage due to PCM administration. The present study suggests that A. kurdicus and A. cinereus possess hepatoprotective activities that could be partly attributed to their antioxidant effects.

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Section: Serum and Liver Biochemical Markerssupporting

confidence: 53%

Hepatoprotective Potential of Astragalus kurdicus and Astragalus cinereus Extracts against Paracetamol Induced Liver Damage in Rats

Yusufoğlu¹,

Soliman²,

Abdel-Rahm³

et al. 2015

Pakistan J. of Biological Sciences

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show abstract

“…Recent findings suggest that the liver itself produces a secretome endowed with anti-inflammatory properties [22]. In this context, a supplement based on liver from healthy animals could be worthy of analysis [39], both as nutraceutical and substrate for studying hepatocyte biology. The aim of this work was the preliminary assessment of the in vitro bioactivity and safety of a freeze-dried liver preparation of porcine origin.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Evaluation of Cytotoxicity and Proliferative Effects of Lyophilized Porcine Liver Tissue on HepG2 Hepatoma Cells and Adipose-Tissue-Derived Mesenchymal Stromal Cells

Berni

Conti

Ferroni³

et al. 2021

Applied Sciences

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In recent years, nutritional supplements from different sources have been widely considered to support medical treatments in patients affected by chronic hepatopathies. Their potential therapeutic benefit has been recognized, but some evidence of safety issues has been reported. Recently it has been hypothesized that the liver could produce various of bioactive factors to maintain organ homeostasis and promote tissue healing. Thus, liver-specific preparations containing bioactive factors could provide a suitable substrate for in vitro study of liver tissue maintenance/healing, as a prospective regenerative medicine approach. Furthermore, they could represent a dietary supplement or nutraceutical for adjuvant therapies when correctly prepared and formulated. This work aims to provide data about the safety and biological activity of a freeze-dried porcine liver preparation. The lyophilized powder obtained from the whole organ has been tested in term of in vitro cell cytotoxicity (MTT assay) and proliferation assays (bromo-deoxyuridine incorporation and direct cell count) in two different cell types: human hepatoma HepG2 cell line and adipose-tissue-derived canine mesenchymal stromal cells (At-MSCs). At concentration levels between 100 to 500 µg/mL, the lyophilized liver powder stimulated mitochondrial metabolism as assessed by MTT assay (p ≤ 0.001 for HepG2 and for At-MSCs) and induced an increase in bromo-deoxyuridine incorporation in both cell types (p ≤ 0.01 for HepG2 and p < 0.001 for At-MSCs). In addition, direct cell count demonstrated a higher proliferative activity in treated At-MSCs (p < 0.001). Although preliminary, these data suggest that the whole-liver powder is noncytotoxic in vitro and may represent a stimulus to cell metabolism and proliferation. Further studies are needed to detect the bioactive components of the supplement and characterize in deeper detail the cellular pathways that they can modulate.

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Therapeutic Effects of Different Animal Bile Powders on Lipid Metabolism Disorders and Their Composition Analysis

Chen

Chu

Lin

et al. 2021

Chin. J. Integr. Med.

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Effects of animal liver and bile extracts on biochemical values of rat ethanol‐induced fatty liver

Cited by 3 publications

References 16 publications

Hepatoprotective Potential of Astragalus kurdicus and Astragalus cinereus Extracts against Paracetamol Induced Liver Damage in Rats

Hepatoprotective Potential of Astragalus kurdicus and Astragalus cinereus Extracts against Paracetamol Induced Liver Damage in Rats

In Vitro Evaluation of Cytotoxicity and Proliferative Effects of Lyophilized Porcine Liver Tissue on HepG2 Hepatoma Cells and Adipose-Tissue-Derived Mesenchymal Stromal Cells

Therapeutic Effects of Different Animal Bile Powders on Lipid Metabolism Disorders and Their Composition Analysis

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