Transpiration is a key process in the application of phytoremediation 'to soil or groundwater pollutants. To be successful, vegetation must transpire . enough water from the soil or groundwater to control or take up the,cdntam-in&. Transpiration is driven by a combination of abiotic (climate, soil water availability, and groundwater depth) and biotic (leaf area, stomatal functions, root amount and distribution, and hydraulic characteristies) that need to be evaluated when considering appropriate site and species combinations. The protocols are not trivial, but transpiration can be measured at a variety of scales using techniques such as direct measurements of sap flow on individual trees, eddy flux gradient analyses, or gauged watersheds. Alternatively, models can be used to estimate transpiration, but these usually require on-site calibration or parameterization to produce accurate predictions. Case study analyses across a range of site conditions and species indicate a maximum transpiration capacity of approximately 7.5 x lo6 liters of water per hectare per year (8 x IO5 gallons of water per acre per year), with a range of 1.5x lo6 to 7.5x lo6 liters per hectare per year (1.6 x 105 to 8x lti gallons per acre per year). Variation among sites is related to species, tree size, and .stocking (i.e., vegetation density) differences. Application of a physiologically based and site-specific parameterized model suggests reasonable agreement between . measured and predicted transpiration estimates for the Air Force Plant 4 site in central Texas.