Effects of anodic oxidation of a substoichiometric titanium dioxide reactive electrochemical membrane on algal cell destabilization and lipid extraction

Hua, Likun; Guo, Lun; Thakkar, Megha; Wei, Dequan; Agbakpe, Michael; Kuang, Liyuan; Magpile, Maraha; Chaplin, Brian P.; Tao, Yi; Shuai, Danmeng; Zhang, Xihui; Mitra, Somenath; Zhang, Wen

doi:10.1016/j.biortech.2015.12.041

Cited by 38 publications

(11 citation statements)

References 35 publications

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“…Membrane lipids and carbohydrates were extracted from the breast cell membranes according to previously reported method [70–72]. For membrane proteins extraction, the breast cell suspension (5 × 10 6 cells) was centrifuged at 1000 rpm for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Pattern-based sensing of triple negative breast cancer cells with dual-ligand cofunctionalized gold nanoclusters

Tao

Auguste

2017

Biomaterials

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Early detection of breast cancer is a critical component in patient prognosis and establishing effective therapy regimens. Here, we developed an easily accessible yet potentially powerful sensor to detect cancer cell targets by utilizing seven dual-ligand cofunctionalized gold nanoclusters (AuNCs) as both effective cell recognition elements and signal transducers. On the basis of this AuNC multichannel sensor, we have successfully distinguished healthy, cancerous and metastatic human breast cells with excellent reproducibility and high sensitivity. Triple negative breast cancer cells (TNBCs), which exhibit low expression of the estrogen receptor, progesterone receptor, and human epidermal growth factor receptor-2, were identified. The high accuracy of the blind breast cell sample tests further validates the practical application of the sensor array. In addition, the versatility of the sensor array is further justified by identifying amongst distinct cell types, different cell concentrations and cell mixtures. Notably, the drug-resistant cancer cells can also be efficiently discriminated. Furthermore, the dual-ligand cofunctionalized AuNCs can efficiently differentiate different cells from the peripheral blood of tumor-free and tumor-bearing mice. Taken together, this fluorescent AuNCs based array provides a powerful cell analysis tool with potential applications in biomedical diagnostics.

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Section: Methodsmentioning

confidence: 99%

Pattern-based sensing of triple negative breast cancer cells with dual-ligand cofunctionalized gold nanoclusters

Tao

Auguste

2017

Biomaterials

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“…REMs, acting as both filters and electrodes, are often made of porous, conductive, and chemically and mechanically stable materials such as boron-doped diamond, Ti/SnO 2 –Sb, Ti 4 O 7 , , and polymer composites with blended conducting materials such as carbon nanotubes (CNTs). , Application of electric potential bias transfers electrons from the conduction band of the REM (anode) to the external circuit and the cathode. The degradation of organic matters by the REM is driven by (1) direct anodic oxidation and (2) indirect electrolysis by radical species. , For example, hydroxyl radicals ( • OH) could be formed via water oxidation at an anode surface when the electric potential is supplied. , Other oxidative species could be produced, including chlorine and hypochlorite, hydrogen peroxide, and ozone as summarized in the Supporting Information.…”

Section: Introductionmentioning

confidence: 99%

Microalgae Filtration Using an Electrochemically Reactive Ceramic Membrane: Filtration Performances, Fouling Kinetics, and Foulant Layer Characteristics

Hua

Cao

et al. 2020

Environ. Sci. Technol.

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Electrochemical membrane filtration has proven to be successful for microbial removal and separation from water. In addition, membrane fouling could be mitigated by electrochemical reactions and electrostatic repulsion on a reactive membrane surface. This study assessed the filtration performances and fouling characteristics of electrochemically reactive ceramic membranes (a Magneli phase suboxide of TiO 2 ) when filtering algal suspension under different dc currents to achieve anodic or cathodic polarization. The critical flux results indicate that when applying positive or negative dc currents (e.g., 1.25−2.5 mA•cm −2 ) to the membrane, both significantly mitigated membrane fouling and thus maintained higher critical fluxes (up to 14.6 × 10 −5 •m 3 •m −2 •s −1 or 526 LMH) compared to the critical flux without dc currents. Moreover, applying dc currents also enhanced membrane defouling processes and recovered high permeate flux better than hydraulic and chemical backwash methods. Moreover, fouling kinetics and the cake layer formation were further analyzed with a resistance-in-series model that revealed many important but underexamined parameters (e.g., cake layer resistance and cake layer thickness). The cake layer structures (e.g., compressibility) were shown to vary with the electrochemical activity, which provide new insight into the biofouling mechanisms. Finally, the algogenic odor, geosmin, was shown to be effectively removed by this reactive membrane under positive dc currents (2.5 mA•cm −2 ), which highlights the multifunctional capabilities of electrochemically reactive membrane filtration in biomass separation, fouling prevention, and pollutant degradation.

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“…Thermoresponsive polymers can obtain a disruption efficiency of about 32% and the combination of polymers with enzymes increased the disruption efficiency to 68% (Zheng et al, 2016). Alternatively, Hua et al (2016) describe the use of a Ti 4 O 7 -based reactive electrochemical membrane for simultaneous harvesting and cell disruption to enhance lipid extraction.…”

Section: Recent Developmentsmentioning

confidence: 99%

Cell disruption technologies

Dhondt

Martín-Juárez

Bolado

et al. 2017

Microalgae-Based Biofuels and Bioproducts

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IntroductionAlgal cell walls separate the inside cell content from the environment to protect the cell against desiccation, pathogens, and predators while still allowing exchange of compounds. Toward application of algae biomass as a sustainable resource, disruption of this cell wall (¼cell disruption) is an essential pretreatment step to maximize product recovery in downstream processes of the algae biorefinery. Also for direct use of algae in feed or food, cell rupture is required to increase the bioavailability of algae constituents. Depending on the cell wall structure, the size, and the shape of algae, cell disruption can be challenging. A variety of cell disruption methods is currently available, and new approaches are being elaborated in parallel. Since downstream processing is responsible for a large part of the operational costs in the whole production chain, cell disruption technologies should be low cost and energy efficient and result preferably in high product quality. This chapter provides information on cell wall types and gives an overview of physical-mechanical and (bio-)chemical cell disruption technologies with attention to development stage, energy efficiency, product quality, costs, emerging approaches, and applicability on large scale. Cell wall types in various groups of microalgae and cyanobacteriaThe cell wall composition and architecture of algae and cyanobacteria are highly variable ranging from tiny membranes to multilayered complex structures. Despite the importance of algal cell wall properties in biotechnology, little structural information is available for most species (Scholz et al., 2014). Based on the complexity of surface structures, four cell types could be distinguished (Barsanti and Gualtieri, 2006;Lee, 2008) (Fig. 6.1). A simple cell membrane (Fig. 6.1, Type 1) is present in short-lived stages (e.g., gametes), chrysophytes, raphidophytes, green algae Dunaliella, and haptophytes Isochrysis. It consists of a lipid bilayer with integrated and peripheral proteins. Sometimes a cap of glycolipids and glycoproteins envelops the outer surface of cell membrane. Cell membranes with additional extracellular material are known in cyanobacteria and many groups of algae, including palmelloid phases. It is the most diverse cell wall type that includes various membrane-associated structures (cell wall, Microalgae-Based Biofuels and Bioproducts. http://dx

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Effects of anodic oxidation of a substoichiometric titanium dioxide reactive electrochemical membrane on algal cell destabilization and lipid extraction

Cited by 38 publications

References 35 publications

Pattern-based sensing of triple negative breast cancer cells with dual-ligand cofunctionalized gold nanoclusters

Pattern-based sensing of triple negative breast cancer cells with dual-ligand cofunctionalized gold nanoclusters

Microalgae Filtration Using an Electrochemically Reactive Ceramic Membrane: Filtration Performances, Fouling Kinetics, and Foulant Layer Characteristics

Cell disruption technologies

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