Effects of anthracene on microbial activities and organic matter decomposition in a Pinus halepensis litter from a Mediterranean coastal area

Qasemian, Leila; Guiral, Daniel; Ziarelli, Fabio; Dang, Thi Kieu Van; Farnet, Anne‐Marie

doi:10.1016/j.soilbio.2011.12.002

Cited by 26 publications

(18 citation statements)

References 46 publications

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“…Laccase has been reported in some well-known ascomycetes from Sordariomycetes class (Kittl et al 2012), such as Myceliophtora thermophila (Berka et al 1997) and Melanocarpus albomyces (Kiiskinen et al 2002). However, the genus Chaetomium is a representative model of cellulolytic filamentous fungi, while few data have been reported as laccase producers (Chefetz et al 1998;Qasemian et al 2012;Manai et al 2016).…”

Section: Resultsmentioning

confidence: 99%

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A halotolerant laccase from Chaetomium strain isolated from desert soil and its ability for dye decolourization

et al. 2017

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A novel fungal laccase produced by the ascomycete sp. isolated from arid soil was purified and characterized and its ability to remove dyes was determined. Extracellular laccase was purified 15-fold from the crude culture to homogeneity with an overall yield of 50% using ultrafiltration and anion-exchange chromatography. The purified enzyme was found to be a monomeric protein with a molecular mass of 68 kDa, estimated by SDS-PAGE, and with an isoelectric point of 5.5. The optimal temperature and pH value for laccase activity toward 2,6-DMP were 60 °C and 3.0, respectively. It was stable at temperatures below 50 °C and at alkaline conditions. Kinetic study showed that this laccase showed higher affinity on ABTS than on 2,6-DMP. Its activity was enhanced by the presence of several metal ions such as Mg, Ca and Zn, while it was strongly inhibited by Fe, Ag and Hg. The novel laccase also showed high, remarkable sodium chloride tolerance. Its ability to decolorize different dyes, with or without HBT (1-hydroxy-benzotriazole), as redox mediator, suggests that this protein may be useful for different industrial applications and/or bioremediation processes.

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Section: Resultsmentioning

confidence: 99%

“…Compared with laccases produced from Chaetomium sp. and Xylogone sphaerospora (two ascomycota), the purified enzyme showed a high tolerance to the presence of sodium chloride ions (Qasemian et al 2012). Qasemian et al (2012) showed that laccases from Chaetomium sp.…”

Section: Effect Of Chloride On Laccase Activity and Stabilitymentioning

confidence: 99%

A halotolerant laccase from Chaetomium strain isolated from desert soil and its ability for dye decolourization

et al. 2017

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“…Thus, the variations in enzyme activities between soil samples showed that in tested soils pollution inputs have specifi c site effects, which may be a result of lower sensitivity of local microbial communities to PAHs and heavy metals. For instance, Qasemian et al (2012) found an increased activity of acid phosphatase, cellulase and β-glucosidase in the leaf litter of forest pine (Pinus halepensis) after 3 months of anthracene contamination. The changes in the activity of an individual enzyme did not allow for a precise quantifi cation of soil degradation by long-time mixed contamination.…”

Section: Enzyme Activitymentioning

confidence: 99%

Activity and functional diversity of microbial communities in long-term hydrocarbon and heavy metal contaminated soils

Markowicz¹,

Płaza²,

Piotrowska‐Seget³

2016

Archives of Environmental Protection

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Abstract:The impacts of long-term polycyclic aromatic hydrocarbons (PAHs) and heavy metal pollution on soil microbial communities functioning were studied in soils taken from an old coke plant. The concentrations of PAHs in the tested soils ranged from 171 to 2137 mg kg -1 . From the group of tested heavy metals, concentrations of lead were found to be the highest, ranging from 57 to 3478 mg kg -1 , while zinc concentrations varied from 247 to 704 mg kg -1 and nickel from 10 to 666 mg kg -1. High dehydrogenase, acid and alkaline phosphatase activities were observed in the most contaminated soil. This may indicate bacterial adaptation to long-term heavy metal and hydrocarbon contamination. However, the Community Level Physiological Profi les (CLPPs) analysis showed that the microbial functional diversity was reduced and infl uenced to a higher extent by some metals (Pb, Ni), moisture and conductivity than by PAHs.

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“…While the ways in which microorganisms mineralize organic contaminants have been extensively documented in studies on bioremediation technologies (Gianfreda and Rao, 2004;Novotný et al, 2004;D'Annibale et al, 2006;Wu et al, 2008), little is known about how far autochthonous microbial communities can withstand PAHs and whether they may be involved in degrading theses pollutants. In our previous study, we showed that anthracene, a model PAH, artificially added in laboratory experiments (Qasemian et al, 2011(Qasemian et al, , 2012 has no toxic effects on microbial activities expressed by whole microbial communities in Pinus halepensis litter from a coastal Mediterranean site. It therefore appeared useful to compare these results obtained from litters in coastal environments with the responses of microbial communities from inland areas.…”

Section: Introductionmentioning

confidence: 96%

Does anthracene affect microbial activities and organic matter decomposition? A comparative study in Pinus halepensis litters from Mediterranean coastal and inland areas

Qasemian¹,

Guiral²,

Ziarelli³

et al. 2012

Chemosphere

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The widespread concern about pollution caused by Polycyclic Aromatic Hydrocarbons (PAHs) raises the question of how they affect soil microbial communities which are potentially involved in the transformation of these pollutants. Using microcosms, we describe the effect of anthracene, a model PAH, on microbial communities inhabiting a Pinus halepensis litter from both coastal (COS) and inland (INL) Mediterranean sites. The microcosms were incubated over 3 months (25°C, 60% WHC) and the effects of anthracene on microbial activities of both litters were monitored. Different enzyme activities (laccase, cellulase, b-glucosidase and acid phosphatase) and microbial respiration were measured and variations in litter chemical composition over incubation were determined using 13 C Nuclear Magnetic Resonance (NMR) from both sites. Our results show that lignocellulolytic enzymes increased markedly after a 3-month incubation in COS microcosms, especially in the presence of anthracene, whereas INL microcosms were not similarly affected. These results show that anthracene not only has no toxic effect on the microbial activities tested but actually enhances the lignocellulolytic activities of the fungal communities from coastal litters, demonstrating the detoxification potential and resistance of stressed Mediterranean coastal ecosystems.

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Effects of anthracene on microbial activities and organic matter decomposition in a Pinus halepensis litter from a Mediterranean coastal area

Cited by 26 publications

References 46 publications

A halotolerant laccase from Chaetomium strain isolated from desert soil and its ability for dye decolourization

A halotolerant laccase from Chaetomium strain isolated from desert soil and its ability for dye decolourization

Activity and functional diversity of microbial communities in long-term hydrocarbon and heavy metal contaminated soils

Does anthracene affect microbial activities and organic matter decomposition? A comparative study in Pinus halepensis litters from Mediterranean coastal and inland areas

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