Effects of Anti‐Immunoglobulin Antibodies on Murine B Lymphocytes and Humoral Immune Responses

Finkelman, Fred D.; Mond, James J.; Woods, Virgil L.; Wilburn, S B; Berning, A K; Sehgal, Evan; Scher, I

doi:10.1111/j.1600-065x.1980.tb00330.x

Cited by 19 publications

(11 citation statements)

References 53 publications

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“…The in vivo immunosuppression generated by anti-b antibodies which we observed extends previous findings that in vitro anti-8 treatment suppresses B cell responses, although less completely than anti+ treatment (reviewed in [19]). Such results clearly demonstrate that surface IgD can transmit the same sort of immunosuppressive signal in response to heterologous anti-heavy chain antibodies as that transmitted by surface IgM.…”

Section: Discussionsupporting

confidence: 85%

Suppression of specific IgM, IgA and IgG responses in mice treated with anti‐6 from birth

Wathen

Manning

1986

Eur J Immunol

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In contrast to previous studies, we report that heterologous anti-delta antibodies can act as a powerful multi-class humoral immunosuppressant. Primary direct plaque-forming cell (IgM) responses of BALB/c mice injected from birth with a rabbit anti-delta antiserum were reduced to 3% of control levels against a T-dependent antigen (sheep red blood cells), and to 2% against a T-independent antigen (dextran); IgA responses against 2 intraduodenal injections of cholera toxin were reduced to 7% of control levels. Other secondary immune responses of anti-delta-suppressed mice were suppressed to a lesser degree. IgM plaque responses generated by 2 injections of sheep red cells, for example, were reduced to 50% of control levels, with reduction of the corresponding IgG response to 42%. The multi-class suppression observed indicates a clonal differentiation pathway in which the IgD+ cell develops into IgM-, IgA- and IgG-secreting cells. In light of reports that the IgD+ cell is antigen sensitive, our demonstration that IgD is capable of delivering the same sort of immunosuppressive signal in response to anti-heavy chain antibodies that IgM delivers also suggests, though it does not establish, an antigen-receptor role for IgD.

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Section: Discussionsupporting

confidence: 85%

Suppression of specific IgM, IgA and IgG responses in mice treated with anti‐6 from birth

Wathen

Manning

1986

Eur J Immunol

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“…The percentage of cIgG: cells in the GaMG-treated group is significantly greater than that in the GaF-treated group at the p < 0.05 level for days 12, 14, and 17 and at the p<O.O1 level for days 8,28, and adult.…”

Section: Effects Of Gamg On Ig Secretionmentioning

confidence: 80%

“…Irnmunol. 1983.13: 900-905 Ontogeny of anti-&induced polyclonal activation 901 purified rabbit antibodies against mouse IgD (RaMG) [5), IgM (RaMp) [6], IgGl (RaMyl) [2], and keyhole limpet hemocyanin (RaKLH) [7], as well as preparation of F(ab'), fragments and fluorescein isothiocyanate (FITC) derivatives of some of these antibodies have also been described [8,91. Monoclonal FITC-anti-Thy-1.2 was purchased from Becton Dickinson (Mountain View, CA), and used at a 1 : 100 dilution.…”

Section: Antibodiesmentioning

confidence: 99%

Polyclonal activation of the murine immune system by an antibody to IgD III. Ontogeny

1983

Self Cite

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It has recently been demonstrated that the injection of adult mice with an affinity-purified goat antibody to mouse IgD (GaM delta) stimulates activation of the humoral immune system that resembles, on a polyclonal level, specific B cell activation by a T cell-dependent antigen. One to 2 days after adult BALB/c mice are injected with 200 micrograms of GaM delta, their splenic B lymphocytes undergo a series of T-independent activation steps that include increases in surface (s) Ia expression, cell size and DNA synthesis. Seven days after GaM delta injection, these cells undergo T-dependent activation steps, that include further proliferation as well as differentiation into IgG1-secreting cells. We have now studied the ontogeny of the T-independent (day 2) and T-dependent (day 7) activation steps by injecting 100-200 micrograms of GaM delta into 3-day- to 10-week-old BALB/c mice. GaM delta failed to induce increases in B cell sIa expression or size 2 days after injection of mice 2 weeks old or younger and failed to stimulate increased DNA synthesis 2 days after injection of 4-week-old mice. In contrast, increases in spleen cell sIa expression, size and DNA synthesis were seen 7 days after injection of 6- to 8-day-old mice. Furthermore, increases in the numbers of spleen cells with large amounts of intracytoplasmic IgG1 were seen at the same time, although these increases were much less than were seen in GaM delta-treated adult mice. Thus, the ability of GaM delta to induce T help and to act in concert with such help to stimulate B cell proliferation and differentiation precedes in ontogeny the ability of GaM delta to directly induce B cell proliferation and early differentiative events. In addition, the early activating events that we have studied are not required for T-dependent B cell proliferation and antibody production to occur, although they appear to contribute to the magnitude of clonal expansion and antibody production.

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“…Studies of wheat (21), Phlox (22), and European Asplenium lepidum (23) have shown that differently named species may represent analogous combinations of different morphotypes of the same diploid progenitor species. Variation in esterase genotypes indicates that Tragopogon mirus, a recent allotetraploid derivative of two species introduced into North America, originated independently at widely separated localities (4). Allozyme data also suggest multiple origins for the fern allotetraploid Pellaea wrightiana (24).…”

mentioning

confidence: 99%

Human Immunoglobulin D: Genomic Sequence of the Delta Heavy Chain

White

Shen

Word

et al. 1985

Science

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The DNA coding for the human immunoglobulin D(IgD) heavy chain (delta, delta) has been sequenced including the membrane and secreted termini. Human delta, like that of the mouse, has a separate exon for the carboxyl terminus of the secreted form. This feature of human and mouse IgD distinguishes it from all other immunoglobulins regardless of species or class. The human gene is different from that of the mouse; it has three, rather than two, constant region domains; and its lengthy hinge is encoded by two exons rather than one. Except for the third constant region, the human and mouse genes are only distantly related.

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Effects of Anti‐Immunoglobulin Antibodies on Murine B Lymphocytes and Humoral Immune Responses

Cited by 19 publications

References 53 publications

Suppression of specific IgM, IgA and IgG responses in mice treated with anti‐6 from birth

Suppression of specific IgM, IgA and IgG responses in mice treated with anti‐6 from birth

Polyclonal activation of the murine immune system by an antibody to IgD III. Ontogeny

Human Immunoglobulin D: Genomic Sequence of the Delta Heavy Chain

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