Exploring the type and amounts of the secondary metabolites (SMs) in a given fodder species was considered as a meaningful act for safe and profitable utilization of that particular feedstuff in the livestock industry. This study was conducted in the Konso zone, southern Ethiopia, to explore the secondary metabolite composition of Commelina species in two seasons and at two different altitudes. Samples were collected from the two altitudes and seasons. A completely randomized design was used in a factorial arrangement (five species (C. benghalensis, C. imberbis, C. diffusa, C. albescens, and C. africana), two altitudes, and two seasons) with three repetitions per treatment. The SM contents of the Commelina species were reasonably influenced by both seasonal and altitudinal changes. The mean alkaloid (3.67%), total phenols (9.76 mg GAE/g), flavonoid (3.81 mg CE/g) and condensed tannin (1.10 mg CE/g) values for the herb species in wet season inclined (p < 0.001) to 7.02%, 14.07 mg GAE/g, 7.68 mg CE/g and 2.38 mg CE/g, respectively, in dry season. The wet season saponin concentration of the species (2.65 g/Kg) significantly decreased (p < 0.001) to 1.28 g/Kg in the dry season. Similarly, the lowland saponin (2.26 g/Kg), alkaloid (3.70%), total phenols (10.89 mg GAE/g), flavonoid (4.71 mg CE/g), and condensed tannin (0.98 mg CE/g) contents were increased (p < 0.01) to 3.03 g/Kg, 5.47%, 13.61 mg GAE/g, 6.37 mg CE/g, and 1.81 mg CE/g, respectively, in the midlands. Alkaloids, total phenols, flavonoids and condensed tannin concentrations showed positive correlations with each other (P<0.05) and with seasonal (P<0.001) and altitudinal changes (P<0.001) as well. The findings of this study suggested that the SM concentrations of Commelina species were within the limits tolerable for ruminants. In conclusion, Commelina species could serve as a safe and beneficial forage herb to boost nutrient intake, improve nutrient use efficiency and hinder methane emissions, for animals consuming them, in areas where they are available in abundance.
